Some platforms offer a low to medium throughput but are more flexible. Although the format may allow only 32 or even 16 samples, thermocycling times are faster and multiple runs can be performed thereby increasing the potential throughput. Performing multiple runs rapidly may be an advantage when several applications are employed which require different cycling parameters. The SmartCycler offers a different concept to real-time PCR of random access, which means independent programming of cycling parameters for 16 different assays. Each reaction vessel has its own element so that runs in available slots may be started any time, whilst other reaction sites are already in use. It is also modular allowing up to six units to be operated by a single computer. Others, such as the Chromo4 offers a modular design for maximum flexibility.
from Logan and Edwards (2009)
in Real-Time PCR: Current Technology and ApplicationsBibliography:
- Real-Time PCR: Current Technology and Applications
- Real-Time PCR in Microbiology: From Diagnosis to Characterization
- PCR Troubleshooting: The Essential Guide
- PCR Books