The polymerase chain reaction (PCR) has been widely used for amplification of DNA sequences. The PCR technique can be coupled with a variety of detection assays for the identification of a wide range of DNA targets. Reducing PCR to the microchip level is of interest for portable detection technologies and high-throughput, massively parallel analytical systems. The basic process of creating a miniaturized, microfluidic PCR chip and a simple method for thermally cycling this microchip during PCR amplification have been described (Herold and Rasooly, 2009. Lab-on-a-Chip Technology. ISBN: 978-1-904455-47-9) These methods can be broadly applied to a variety of microchip architectures, materials, and downstream analytical methods.

Labels: PCR chips, PCR on a chip