qPCR Data Analysis: Unlocking the Secret to Successful Results
from Jan Hellemans and Jo Vandesompele writing in PCR Troubleshooting and Optimization: The Essential Guide

Real-time quantitative PCR (qPCR) is the gold standard for fast, accurate, sensitive and cost-efficient gene expression analysis. Despite its conceptual simplicity and ease of use, the multi-step qPCR workflow contains many potential pitfalls. An intelligent experiment design and setup, high quality reagents and assays, quality controls in each step of the workflow, proper quantification models and appropriate bio-statistical analyses pave the way to successful gene expression results. Data analysis aspects include the evaluation of pilot studies and quality controls, through universally applicable quantification models and bio-statistics, to the reporting of experiment results.

Further reading: PCR Troubleshooting and Optimization: The Essential Guide

Key words: Real-time quantitative PCR, Gene expression analysis, Multi-step qPCR, Multi-step qPCR workflow, PCR quality control, PCR quantification models, CR bio-statistical analyses, Gene expression, PCR Data analysis, PCR quality controls, PCR quantification, qPCR