Salmonellae
PCR in Salmonella classification
Category: Applications
PCR in sub-species level Salmonella classification
from Burkhard Malorny, Elisabeth Hauser and Ralf Dieckmann writing in Salmonella: From Genome to Function
Salmonellae form a complex group of bacteria consisting of two species, 6 subspecies and more than 2,500 serovars (serotypes). Salmonella identification below species level is most often limited to phenotypic typing methods such as biochemical and serological identification, which are costly, time-consuming and do not always reflect the evolution of Salmonella groups. Newer methods for Salmonella typing and subtyping include (multiplex-) PCR-based methods. In recent years further molecular typing technologies were evaluated for this purpose. A recent review discusses some of these emerging technologies. These new techniques promise significant advantages compared to traditional culture-based methods with respect to speed, ease of use, reliability and automation.
Further reading: Salmonella: From Genome to Function
from Burkhard Malorny, Elisabeth Hauser and Ralf Dieckmann writing in Salmonella: From Genome to Function
Salmonellae form a complex group of bacteria consisting of two species, 6 subspecies and more than 2,500 serovars (serotypes). Salmonella identification below species level is most often limited to phenotypic typing methods such as biochemical and serological identification, which are costly, time-consuming and do not always reflect the evolution of Salmonella groups. Newer methods for Salmonella typing and subtyping include (multiplex-) PCR-based methods. In recent years further molecular typing technologies were evaluated for this purpose. A recent review discusses some of these emerging technologies. These new techniques promise significant advantages compared to traditional culture-based methods with respect to speed, ease of use, reliability and automation.
Further reading: Salmonella: From Genome to Function