A new book on PCR Troubleshooting and Optimization has been announced by Caister Academic Press. Included in the book is: Strategies for preparing effective controls and standards for PCR, when they should be employed and how to interpret the information they provide. The significance of optimization for efficiency, precision and sensitivity of PCR methodology and essential guidance on how to troubleshoot inefficient reactions. Design and optimization techniques, the use of appropriate controls, the significance of standard curves and the principles and strategies required for effective troubleshooting. The importance of sample preparation and quality, primer design, controlling inhibitors, avoiding amplicon and environmental contamination, optimizing reagent quality and concentration, and modifying the thermal cycling protocol for optimal sensitivity and specificity.

Further reading: PCR Troubleshooting and Optimization: The Essential Guide

Key words: Standards for PCR, Controls for PCR, Troubleshooting PCR, Optimization of PCR