The majority of instrument manufacturers supply optimised real-time PCR mastermixes, these reagents benefit from being quality controlled, are easy to use, and usually offer reproducible and reliable results. However, for certain laboratories the additional cost can be prohibitive, however with an array of third party companies (e.g. Qiagen, Eurogentec, Invitrogen) now supplying real-time reagents, competition in this market should lead to reduced costs. Target specific kits for a range of applications are available from some manufacturers and other companies (e.g. Roche, Applied Biosystems, Qiagen). Additionally, Applied Biosystems offer Assays-on-Demand and Assays-by-Design services for SNP genotyping by real-time PCR. A number of companies now offer real-time PCR services from primer design right through to assay design and validation, quality assurance and other custom services.
from Logan and Edwards (2009)
in Real-Time PCR: Current Technology and ApplicationsBibliography:
- Real-Time PCR: Current Technology and Applications
- Real-Time PCR in Microbiology: From Diagnosis to Characterization
- PCR Troubleshooting: The Essential Guide
- PCR Books
Labels: Applied Biosystems, Eurogentec, Invitrogen, PCR mastermixes, Qiagen