A one-step real-time RT-PCR helps to identify mixed rotavirus infections in Mexico.

A one-step real-time RT-PCR helps to identify mixed rotavirus infections in Mexico.

Rotaviruses proceed being crucial pathogens accountable of diarrhea in younger kids worldwide. Seminested reverse transcription polymerase chain response (RT-PCR) is used to find out rotavirus genotype; nevertheless, this method employs multistep procedures. The actual-time RT-PCR is a quick and dependable device that can be utilized as rotavirus genotyping device, particularly in rotavirus outbreaks. On this […]

Development of a New Internally Controlled One-Step Real-Time RT-PCR for the Molecular Detection of Enterovirus A71 in Africa and Madagascar

Development of a New Internally Controlled One-Step Real-Time RT-PCR for the Molecular Detection of Enterovirus A71 in Africa and Madagascar

Enterovirus A71 (EV-A71) is a number one reason behind hand-foot-and-mouth illness (HFMD) and might be related to extreme neurological problems. EV-A71 strains might be labeled into seven genogroups, A-H, on the idea of the VP1 capsid protein gene sequence. Genogroup A contains the prototype pressure; genogroups B and C are accountable of main outbreaks worldwide, […]

Microdroplet-based one-step RT-PCR for ultrahigh throughput single-cell multiplex gene expression analysis and rare cell detection

Microdroplet-based one-step RT-PCR for ultrahigh throughput single-cell multiplex gene expression analysis and rare cell detection

Gene expression evaluation of particular person cells permits characterization of heterogeneous and uncommon cell populations, but widespread implementation of current single-cell gene evaluation strategies has been hindered because of limitations in scale, ease, and price. Right here, we current a novel microdroplet-based, one-step reverse-transcriptase polymerase chain response (RT-PCR) platform and exhibit the detection of three […]

A one-step real-time RT-PCR assay for simultaneous typing of SARS-CoV-2 mutations associated with the E484K and N501Y spike protein amino-acid substitutions

A one-step real-time RT-PCR assay for simultaneous typing of SARS-CoV-2 mutations associated with the E484K and N501Y spike protein amino-acid substitutions

The emergence of SARS-CoV-2 mutations ensuing within the S protein amino-acid substitutions N501Y and E484Ok, which have been related to enhanced transmissibility and immune escape, respectively, necessitates speedy actions, for which their speedy identification is essential. For the simultaneous typing of each of those mutations of concern (MOCs), a one-step real-time RT-PCR assay using 4 […]

‘Infected vaccinees can also transmit delta variant’

People who have been vaccinated with the Pfizer or AstraZeneca vaccines and who become infected with the delta variant of the coronavirus can transmit the virus, as can unvaccinated people. Also, the effectiveness of vaccines diminishes after a few months, according to large-scale research from the University of Oxford. Someone who has been vaccinated and […]

What is the difference between a PCR test and an antigen test?

In order to be able to go on holiday abroad this summer, you need a negative PCR test or antigen test for many countries. What are these tests? And what is the difference between those two tests? In a polymerase chain reaction (PCR) test, a swab of mucus is taken from the nose and throat with a […]