The development of instruments that allow real-time monitoring of fluorescence within a PCR reaction vessel led to a significant advance in PCR technology and applications. Many different instruments and fluorescent probe systems have been developed…

Standard PCR requires the identification of the amplified fragment(s) by post-PCR analysis, usually by gel electrophoresis. These methods rely on either the size or sequence of the amplicon. Gel electrophoresis, often used to measure the…

There are two general approaches used to obtain a fluorescent signal from the synthesis of product in Real-time PCR. The first depends upon the property of fluorescent dyes such as SYBR Green I to bind to…

This essential manual presents a comprehensive guide to the most up-to-date technologies and applications as well as providing an overview of the theory of this increasingly important technique. This timely and authoritative volume describes the…