A one-step real-time RT-PCR helps to identify mixed rotavirus infections in Mexico.

A one-step real-time RT-PCR helps to identify mixed rotavirus infections in Mexico.

Rotaviruses proceed being crucial pathogens accountable of diarrhea in younger kids worldwide. Seminested reverse transcription polymerase chain response (RT-PCR) is used to find out rotavirus genotype; nevertheless, this method employs multistep procedures. The actual-time RT-PCR is a quick and dependable device that can be utilized as rotavirus genotyping device, particularly in rotavirus outbreaks. On this […]

A one-step real-time RT-PCR assay for simultaneous typing of SARS-CoV-2 mutations associated with the E484K and N501Y spike protein amino-acid substitutions

A one-step real-time RT-PCR assay for simultaneous typing of SARS-CoV-2 mutations associated with the E484K and N501Y spike protein amino-acid substitutions

The emergence of SARS-CoV-2 mutations ensuing within the S protein amino-acid substitutions N501Y and E484Ok, which have been related to enhanced transmissibility and immune escape, respectively, necessitates speedy actions, for which their speedy identification is essential. For the simultaneous typing of each of those mutations of concern (MOCs), a one-step real-time RT-PCR assay using 4 […]