A one-step real-time RT-PCR assay for simultaneous typing of SARS-CoV-2 mutations associated with the E484K and N501Y spike protein amino-acid substitutions

A one-step real-time RT-PCR assay for simultaneous typing of SARS-CoV-2 mutations associated with the E484K and N501Y spike protein amino-acid substitutions

The emergence of SARS-CoV-2 mutations ensuing within the S protein amino-acid substitutions N501Y and E484Ok, which have been related to enhanced transmissibility and immune escape, respectively, necessitates speedy actions, for which their speedy identification is essential. For the simultaneous typing of each of those mutations of concern (MOCs), a one-step real-time RT-PCR assay using 4 locked nucleic acid (LNA) modified TaqMan probes was developed.

The assay is very delicate with a LOD of 117 copies/response, amplification efficiencies >94 % and a linear vary of over 5 log10 copies/response. Validation of the assay utilizing recognized SARS-CoV-2-positive and destructive samples from human and animals revealed its capacity to appropriately determine wild sort strains, and strains possessing both one or each focused amino-acid substitutions, thus comprising a helpful pre-screening instrument for speedy MOC identification. The fundamental ideas of the methodology for the event of the assay are defined so as to facilitate the speedy design of comparable assays in a position to detect rising MOCs.

To regulate circulating vaccine-derived sort 2 poliovirus outbreaks, a extra genetically steady novel Oral Poliovirus Vaccine sort 2 (nOPV2) was developed by focused modifications of Sabin 2 genome. Since the usage of OPV2 made from Sabin 2 pressure has been stopped, it is very important exclude the chance that batches of nOPV2 are contaminated with Sabin 2 virus. Right here, we report the event of a easy quantitative one-step reverse-transcription polymerase chain response assay for the detection and quantitation of Sabin 2 virus within the presence of overwhelming quantities of nOPV2 pressure.

The tactic is particular and linear inside eight log10 vary even within the presence of related quantities of nOPV2 virus. It’s delicate, with a decrease restrict of detection of 0.2 CCID50/mL (an equal of 198 genome copies per mL), and generates reproducible outcomes. This assay can be utilized for high quality management and lot launch of the nOPV2.

Common primers for speedy detection of six pospiviroids in Solanaceae vegetation utilizing onestep RTPCR and RT-LAMP

A lot of viruses and viroids infect solanaceous vegetation inflicting extreme yield losses. A number of seed-borne viroids are at the moment listed as quarantine pathogens in lots of international locations. Amongst them, columnea latent viroid (CLVd), pepper chat fruit viroid (PCFVd), potato spindle tuber viroid (PSTVd), tomato apical stunt viroid (TASVd), tomato chlorotic dwarf viroid (TCDVd), and tomato planta macho viroid (TPMVd) are of main issues. The target of this examine was to design and take a look at common primers that could possibly be used to detect six viroids in solanaceous vegetation utilizing one-step RT-PCR and reverse transcription loop-mediated isothermal amplification (RT-LAMP).

Outcomes revealed {that a} pair of degenerate primers could possibly be utilized in a one-step RT-PCR to amplify six pospiviroids from Solanaceae seeds and vegetation. Furthermore, 5 primers had been designed and utilized in RT-LAMP to amplify six pospiviroids. The minimal focus of viroid RNA required for a profitable detection assorted, starting from one femtogram to 10 nanograms, relying on the species of viroid and detection technique. On the whole, RT-LAMP was extra delicate than RT-PCR however each assays had been speedy and extremely delicate instruments to detect six pospiviroids. Detection strategies at the moment in use for these viroids require at the very least two completely different units of primers. The assays developed on this analysis may facilitate to display screen a lot of solanaceous vegetation and seeds meant for import and export.

A one-step real-time RT-PCR assay for simultaneous typing of SARS-CoV-2 mutations associated with the E484K and N501Y spike protein amino-acid substitutions

Comparability of a onestep real-time RTPCR and a nested real-time RTPCR for a genogroup II norovirus reveals variations in sensitivity relying upon assay design and visualization

Human norovirus (NoV) is the main explanation for acute viral gastroenteritis and a significant supply of foodborne sickness. Detection of NoV in meals and environmental samples is often carried out utilizing molecular methods, together with real-time reverse transcription polymerase chain response (RT-PCR) and fewer often, nested real-time PCR. On this examine, we performed a managed comparability of two revealed NoV detection assays: a broadly reactive one-step real-time RT-PCR and a two-step nested real-time PCR assay. A 20% human fecal suspension containing a genogroup II human NoV was serially diluted, genome extracted, and subjected to amplification utilizing the 2 assays in contrast through PCR Items.

Further amplicon affirmation was carried out by dot blot hybridization utilizing digoxigenin (DIG)-labeled oligonucleotide probes. Each assays displayed comparable amplification commonplace curves/amplification efficiencies; nonetheless, the nested assay persistently detected one log10 decrease virus. Dot blot hybridization improved the detection restrict of the nested real-time PCR by one log10 NoV genome copies however impaired the detection restrict of the one-step real-time RT-PCR by one log10 NoV genome copies. These outcomes illustrate the complexities in designing and deciphering molecular methods having a adequate detection restrict to detect low ranges of viruses that may be anticipated in contaminated meals and environmental samples.

AzuraQuant cDNA Synthesis Kit - 100 Reactions

AZ-1996 100 Reactions
EUR 446.4

AzuraQuant cDNA Synthesis Kit - 25 Reactions

AZ-1995 25 Reactions
EUR 183.6

Axygen Polyester ultra clear sealing film for real time PCR

AXY2152 PK100
EUR 382.8

Eztime Real - time PCRPremix 2x,Taqman

FYT105-100P 100 Preps 1.25 ml Ask for price

Eco 48 Real Time PCR system including software and loading dock

MOL6348 EACH
EUR 18466.8

Eztime Real - time PCRPremix 2x,SYBR Green

FYT103-100P 100 Preps 1.25 ml Ask for price

Eztime Real - time PCRPremix 2x,SYBR Green

FYT103-400P 400 Preps 1.25 ml x 4 Ask for price

Eztime Real - time PCRPremix 2x,Taqman, ROX

FYT105-400P 400 Preps 1.25 ml x 4 Ask for price

Eztime Real - time PCRPremix 2x,Taqman, ROX

FYT106-100P 100 Preps 1.25 ml Ask for price

Eztime Real - time PCRPremix 2x,SYBRGreen , ROX

FYT104-100P 100 Preps 1.25 ml Ask for price

Eztime Real - time PCRPremix 2x,SYBRGreen , ROX

FYT104-400P 400 Preps 1.25 ml x 4 Ask for price

hsa-mir-521 Real-time RT-PCR Detection Kit

20-abx097600
  • EUR 661.20
  • EUR 944.40
  • EUR 477.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye

MB1004-100Reactions 100 Reactions
EUR 392.4

Novel Coronavirus COVID-19 (2019-nCoV) Real Time Multiplex RT-PCR Kit (Detection for 3 Genes )

RR-0479-02 25 tests/kit
EUR 469.56
Description: Novel Coronavirus (2019-nCoV) Real Time Multiplex RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. It detects N gene, E gene and RdRp gene of 2019-nCoV. RR-0479-02 has been also approverd by CFDA for emergency use and is WHO standard.

Azura PureView 1 Kb DNA Ladder - 100 Lanes

AZ-1101 100 Lanes
EUR 140.4

Azura PureView 1 Kb DNA Ladder - 500 Lanes

AZ-1105 500 Lanes
EUR 277.2

Azura PureView 1 Kb DNA Ladder - 1000 Lanes

AZ-1105-2 1000 Lanes
EUR 326.4

Azura PureView 250bp DNA Ladder - 100 Lanes

AZ-1121 100 Lanes
EUR 140.4

Azura PureView 250bp DNA Ladder - 500 Lanes

AZ-1125 500 Lanes
EUR 277.2

Azura PureView 250bp DNA Ladder - 1000 Lanes

AZ-1125-2 1000 Lanes
EUR 326.4

Azura PureView 100bp DNA Ladder - 100 Lanes

AZ-1131 100 Lanes
EUR 140.4

Azura PureView 100bp DNA Ladder - 500 Lanes

AZ-1135 500 Lanes
EUR 277.2

Azura PureView 100bp DNA Ladder - 1000 Lanes

AZ-1135-2 1000 Lanes
EUR 326.4

Azura PureView 50bp DNA Ladder - 100 Lanes

AZ-1151 100 Lanes
EUR 140.4

Azura PureView 50bp DNA Ladder - 500 Lanes

AZ-1155 500 Lanes
EUR 277.2

Azura PureView 50bp DNA Ladder - 1000 Lanes

AZ-1155-2 1000 Lanes
EUR 326.4

AzuraView GreenFast qPCR Blue Mix Lo Rox - 200 Rxn

AZ-2301 200 Rxn
EUR 214.8

AzuraView GreenFast qPCR Blue Mix Lo Rox - 500 Rxn

AZ-2305 500 Rxn
EUR 384

AzuraView GreenFast qPCR Blue Mix Lo Rox - 2000 Rxn

AZ-2320 2000 Rxn
EUR 1195.2

AzuraView GreenFast qPCR Blue Mix Lo Rox - 5000 Rxn

AZ-2325 5000 Rxn
EUR 2817.6

AzuraView GreenFast qPCR Blue Mix Hi Rox - 200 Rxn

AZ-2401 200 Rxn
EUR 214.8

AzuraView GreenFast qPCR Blue Mix Hi Rox - 500 Rxn

AZ-2405 500 Rxn
EUR 384

AzuraView GreenFast qPCR Blue Mix Hi Rox - 2000 Rxn

AZ-2420 2000 Rxn
EUR 1195.2

AzuraView GreenFast qPCR Blue Mix Hi Rox - 5000 Rxn

AZ-2421 5000 Rxn
EUR 2817.6

Mouse Gdnf Signaling Primer Library

MGDNF-I 1 set
EUR 657.6

Mouse Glucose Metabolism Primer Library

MGLM-I 1 set
EUR 657.6

Mouse Hippo Signaling Primer Library

MHPO-I 1 set
EUR 657.6

Mouse Hypoxia Signaling Primer Library

MHYP-I 1 set
EUR 657.6

Mouse Notch Signaling Primer Library

MNOT-I 1 set
EUR 657.6

Mouse Neurotransmitter Signaling Primer Library

MNTS-I 1 set
EUR 657.6

Mouse Integrin Signaling Primer Library

MINT-I 1 set
EUR 891.6

Mouse Oxidative Stress Primer Library

MOSL-I 1 set
EUR 657.6

Mouse Oncogene Primer Library

MONC-I 1 set
EUR 657.6

Mouse Apoptosis Primer Library

MPA-I 1 set
EUR 540

Mouse Ras Signaling Primer Library

MRAS-I 1 set
EUR 657.6

Mouse T-Cell Receptor Signaling Primer Library

MTCR-I 1 set
EUR 657.6

Mouse TGF Beta Signaling Primer Library

MTGFB-I 1 set
EUR 657.6

Mouse Tumor Invasion/Metastasis Primer Library

MTIM-I 1 set
EUR 540

Mouse Tumor Immunity Primer Library

MTIMU-I 1 set
EUR 657.6

Mouse p53 Signaling Primer Library

MTPS-I 1 set
EUR 540

Mouse WNT Signaling Primer Library

MWNT-I 1 set
EUR 657.6

Mouse Toll-like Receptor Signaling Primer Library

newitem 1 set
EUR 657.6

Mouse PI3K-AKT Signaling Primer Library

MAKT-I 1 set
EUR 657.6

Mouse Angiogenesis Primer Library

MAPL-I 1 set
EUR 540

Mouse Adherin - Tight Junction Primer Library

MATJ-I 1 set
EUR 657.6

Mouse Autophagy Primer Library

MATPL-I 1 set
EUR 540

Mouse Bdnf Signaling Primer Library

MBDNF-I 1 set
EUR 657.6

Mouse BMP Signaling Primer Library

MBMP-I 1 set
EUR 657.6

Mouse Cytokine Primer Library I

MCA-I 1 set
EUR 540

Mouse Cytokine Primer Library II

MCA-II 1 set
EUR 540

Mouse Cell Cycle Primer Library

MCC-I 1 set
EUR 540

Mouse Cytokine and Chemokine Receptor Primer Library

MCCR-I 1 set
EUR 774

Mouse CREB Signaling Primer Library

MCREB-I 1 set
EUR 657.6

Mouse DNA Damage Signaling Primer Library

MDDS-I 1 set
EUR 657.6

Mouse DNA Repair Primer Library I

MDRL-I 1 set
EUR 774

Mouse DNA Repair Primer Library II

MDRL-II 1 set
EUR 774

Mouse Adipogenesis Primer Library

MADG-I 1 set
EUR 657.6

Mouse EGFR Signaling Primer Library

MEGFR-I 1 set
EUR 657.6

Mouse Epithelial-Mesenchymal Transition Primer Library

MEMT-I 1 set
EUR 657.6

Mouse Estrogen Receptor Signaling Library

MESR-I 1 set
EUR 774

Mouse Fatty Liver Signaling Primer Library

MFTL-I 1 set
EUR 657.6

Mouse Jak/Stat Signaling Primer Library

MJAK-I 1 set
EUR 774

Mouse MAP Kinase Signaling Primer Library

MMAP-I 1 set
EUR 657.6

Mouse Myeloid Derived Suppressor Cell Primer Library

MMDSC-I 1 set
EUR 657.6

Mouse NFKappaB Primer Library

MNFKB-I 1 set
EUR 540

Human DNA Repair Primer Library I

HDRL-I 1 set
EUR 657.6

Human DNA Repair Primer Library II

HDRL-II 1 set
EUR 657.6

Human Drug Transporter I Primer Library

HDTP-I 1 set
EUR 657.6

Human Drug Detoxication I

HDTX-I 1 set
EUR 657.6

Human Drug Detoxication II

HDTX-II 1 set
EUR 774

Human EGFR Signaling Primer Library

HEGFR-I 1 set
EUR 657.6

Human Epithelial Mesenchymal Transition Primer Library

HEMT-I 1 set
EUR 657.6

Human Endothelial Cell Primer Library

HEND-I 1 set
EUR 657.6

Human ERBB Signaling Primer Library

HERBB-I 1 set
EUR 657.6

Human Estrogen Signaling Primer Library

HESR-I 1 set
EUR 774

Human Focal Adhesion Signaling Primer Library

HFCA-I 1 set
EUR 657.6

Human FGF Receptor Signaling Primer Library

HFGF-I 1 set
EUR 774

Human Fatty Acid and Lipid Metabolism Primer Library

HFLM-I 1 set
EUR 657.6

Human Gastric Cancer Primer Library

HGCPL-I 1 set
EUR 657.6

Human GDNF Signaling Primer Library

HGDNF-I 1 set
EUR 657.6

Human Glucose Metabolism Primer Library

HGLM-I 1 set
EUR 540

Human G-Protein Coupled Receptor Signaling Primer Library

HGPCR-I 1 set
EUR 657.6

Human Hedgehog Signaling Primer Library

HHOG-I 1 set
EUR 774

Human Homeobox Gene Primer Library

HHOX-I 1 set
EUR 774

Human Hippo Signaling Primer Pathway

HHPO-I 1 set
EUR 657.6

Human Heat Shock Protein Primer Library

HHSP-I 1 set
EUR 891.6

Human Hypoxia Signaling Primer Library

HHYP-I 1 set
EUR 657.6

Human Interferon Type I Signaling Primer Library

HIFN-I 1 set
EUR 657.6

Human Interferon Type II Signaling Primer Library

HIFN-II 1 set
EUR 657.6

Human IL17 Signaling Primer Library

HIL17-I 1 set
EUR 657.6

Human Insulin Signaling Primer Library

HINS-I 1 set
EUR 657.6

Human Integrin Signaling Primer Library

HINT-I 1 set
EUR 657.6

Human Actin Cytoskeleton Signaling Primer Library

HACS-I 1 set
EUR 657.6

Human Adipogenesis Primer Library

HADG-I 1 set
EUR 657.6

Human Adaptive & Innate Immune Response Primer Library

HAIIR-I 1 set
EUR 657.6

Human PI3K-AKT Signaling Primer Library

HAKT-I 1 set
EUR 540

Human Amino Acid Metabolism Primer Library

HAMM-I 1 set
EUR 774

Human Androgen Signaling Primer Library

HAND-I 1 set
EUR 657.6

Human Angiogenesis Primer Library

HAPL-I 1 set
EUR 540

Human Antigen Processing and Presentation Primer Library

HAPP-I 1 set
EUR 657.6

Human Atherosclerosis Primer Library

HATH-I 1 set
EUR 657.6

Human Adherin - Tight Junction Primer Library

HATJ-I 1 set
EUR 657.6

Human Autophagy Primer Library

HATPL-I 1 set
EUR 540

Human Alzheimer's Disease Primer Library

HAZD-I 1 set
EUR 657.6

Human B-Cell Receptor Signaling Primer Library

HBCR-I 1 set
EUR 657.6

Human BDNF Signaling Primer Library

HBDNF-I 1 set
EUR 657.6

Human Bladder Cancer Primer Library

HBLCP-I 1 set
EUR 657.6

Human BMP Signaling Primer Library

HBMP-I 1 set
EUR 657.6

Human Brain Cancer Primer Library

HBRC-I 1 set
EUR 657.6

Human Breast Cancer Signaling Primer Library

HBRCA-I 1 set
EUR 657.6

Human Cytokine Primer Library I

HCA-I 1 set
EUR 540

Human Cytokine Primer Library II

HCA-II 1 set
EUR 540

Human Cell Adhesion Primer Library

HCAD-I 1 set
EUR 774

Human cAMP Signaling Primer Library

HCAMP-I 1 set
EUR 657.6

Human Cancer Pathway Primer Library

HCAN-I 1 set
EUR 657.6

Human Calcium Regulation Pathway

HCAR-I 1 set
EUR 657.6

Human Cell Cycle Primer Library

HCC-I 1 set
EUR 540

Human Colon Cancer Primer Library I

HCCP-I 1 set
EUR 657.6

Human Colon Cancer Primer Library II

HCCP-II 1 set
EUR 657.6

Human Cytokine and Chemokine Receptor Primer Library

HCCR-I 1 set
EUR 774

Human Cancer Driver Gene I Primer Library

HCDG-I 1 set
EUR 774

Human Cancer Driver Gene II Primer Library

HCDG-II 1 set
EUR 774

Human Chromatin Regulation Primer Library

HCHR-I 1 set
EUR 774

Human Oncogene circRNA Library

HCirc-Onc-I 1 set
EUR 1125.6

Human CREB Signaling Primer Library

HCREB-I 1 set
EUR 657.6

Human JAK/STAT Signaling Primer Library

HJAK-I 1 set
EUR 657.6

Human Kinase Library I

HKIN-I 1 set
EUR 540

Human Kinase Library II

HKIN-II 1 set
EUR 540

Human Kinase Library III

HKIN-III 1 set
EUR 540

Human Kinase Library IV

HKIN-IV 1 set
EUR 540

Human Kinase Library (I, II, III and IV)

HKIN-X 1 set
EUR 1710

Human Liver Cancer Primer Library

HLCPL-I 1 set
EUR 657.6

Human Ligand Gated Ion Channels Primer Library

HLGI-I 1 set
EUR 891.6

Human MAP Kinase Signaling Library

HMAP-I 1 set
EUR 657.6

Human Myeloid Derived Suppressor Cell Primer Library

HMDSC-I 1 set
EUR 657.6

Human Melanoma Primer Library

HMEL-I 1 set
EUR 657.6

Human Necroptosis Primer Library

HNCR-I 1 set
EUR 657.6

Human NFKappaB Primer Library

HNFKB-I 1 set
EUR 540

Human NK Cell Mediated Toxicity Primer Library

HNKT-I 1 set
EUR 657.6

Human Nitric Oxide Signaling Primer Library

HNOS-I 1 set
EUR 657.6

Human Notch Signaling Pathway

HNOT-I 1 set
EUR 657.6

Human Neurotransmitter Signaling Primer Library

HNTS-I 1 set
EUR 657.6

Human Oncogene Primer Library

HONC-I 1 set
EUR 657.6

Human Oxidative Stress Primer Library

HOSL-I 1 set
EUR 540

Human Osteogenesis Primer Library

HOST-I 1 set
EUR 657.6

Human Ovarian Cancer Primer Library

HOVCL-I 1 set
EUR 657.6

Human Apoptosis Primer Library

HPA-I 1 set
EUR 540

Human Pancreatic Cancer Primer Library

HPANC-I 1 set
EUR 657.6

Human PDGF Signaling Primer Library

HPDGF-I 1 set
EUR 657.6

Human Parkinson's Disease Primer Library

HPKD-I 1 set
EUR 657.6

Human Prostate Cancer Primer Library

HPRC-I 1 set
EUR 657.6

Human Ras Signaling Primer Library

HRAS-I 1 set
EUR 657.6

Human Retinoblastoma Gene Primer Library

HRBG-I 1 set
EUR 657.6

Human Rho GTPase-Signaling Primer Library

HRGTP-I 1 set
EUR 774

Human Cytoskeleton Primer Library

HSCK-I 1 set
EUR 774

Human Stem Cell Primer Library

HSCL-I 1 set
EUR 540

 Extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) an infection has quickly unfold worldwide from the start of 2020. Quantitative reverse transcription-PCR (RT-qPCR) is, to at the present time, the popular methodology for viral RNA detection, even when not with out issues. To beat a few of the limitations nonetheless current for the detection and quantification of nucleic acids in numerous purposes, the usage of one-step reverse transcription-droplet digital PCR (RT-ddPCR) has been established.

Leave a Reply

Your email address will not be published. Required fields are marked *