A one-step real-time RT-PCR helps to identify mixed rotavirus infections in Mexico.

A one-step real-time RT-PCR helps to identify mixed rotavirus infections in Mexico.

Rotaviruses proceed being crucial pathogens accountable of diarrhea in younger kids worldwide. Seminested reverse transcription polymerase chain response (RT-PCR) is used to find out rotavirus genotype; nevertheless, this method employs multistep procedures. The actual-time RT-PCR is a quick and dependable device that can be utilized as rotavirus genotyping device, particularly in rotavirus outbreaks. On this research, we examined a real-time RT-PCR to establish rotavirus genotype utilizing a panel of 252 samples from sufferers with diarrheal illness brought on by G9P[4] and G12P[8] genotypes, which had been recognized as rising rotaviruses in 2 outbreaks in Chiapas, Mexico.

Our outcomes present that the real-time RT-PCR assay detected these rotaviruses, and it allowed us to establish combined genotype infections, G/P mixtures, and the viral abundance in some samples wherein the seminested assay couldn’t establish them. Subsequently, the real-time RT-PCR is a molecular device that may be nice assist throughout rotavirus outbreaks. A one-step multiplex quantitative reverse transcription polymerase chain response protocol is described, for the detection in pome timber of Pear blister canker viroid and Apple scar pores and skin viroid, along with common detection of phytoplasmas.

Whole nucleic acids extraction is carried out in accordance with a modified CTAB protocol and TaqMan MGB probes are used to surpass excessive genetic variability of viroids. The multiplex real-time assay is no less than ten instances extra delicate than typical protocols and its options make it appropriate for fast and large screening of pome fruit timber phytoplasmas and viroids in certification schemes and surveys.

An improved quantitative multiplex one-step RT-PCR (qmosRT-PCR) for simultaneous identification and quantitation of all three serotypes of poliovirus is described. It’s primarily based on utilizing serotype-specific primers and fluorescent TaqMan oligonucleotide probes. The assay can be utilized for high-throughput screening of samples for the presence of poliovirus, poliovirus surveillance and for analysis of virus shedding by vaccine recipients in scientific trials to evaluate mucosal immunity. It might change typical strategies primarily based on cell tradition virus isolation adopted by serotyping. The assay takes solely few hours, and was discovered to be easy, particular, delicate and has giant quantitative linearity vary. As well as, the strategy may very well be used as readout in PCR-based poliovirus titration and neutralization assays.

Growth of a onestep RTPCR assay for detection of pancoronaviruses (α-, β-, γ-, and δ-coronaviruses) utilizing newly designed degenerate primers for porcine and avian `fecal samples.

Coronaviruses (CoVs) are crucial human and animal pathogens due to their potential to trigger extreme epidemics of respiratory or enteric illnesses. In pigs, the newly emerged porcine deltacoronavirus (PDCoV) and re-emerged porcine epidemic diarrhea virus (PEDV) reported within the US and Asia, in addition to the invention of novel CoVs in wild bats or birds, has necessitated growth of improved detection and management measures for these CoVs. As a result of the earlier pancoronavirus (panCoV) RT-PCR established in our laboratory in 2007-2011 didn’t detect deltacoronaviruses (δ-CoVs) in swine fecal and serum samples, our aim was to develop a brand new panCoV RT-PCR assay to detect recognized human and animal CoVs, together with δ-CoVs.

On this research, we designed a brand new primer set to amplify a 668 bp-region inside the RNA-dependent RNA polymerase (RdRP) gene that encodes probably the most conserved protein area of α-, β-, γ-, and δ-CoVs. We established a one-step panCoV RT-PCR assay and standardized the assay situations. The newly established panCoV RT-PCR assay was demonstrated to have a excessive sensitivity and specificity. Utilizing a panel of 60 swine organic samples (feces, intestinal contents, and sera) characterised by PEDV, PDCoV and transmissible gastroenteritis virus-specific RT-PCR assays, we demonstrated that sensitivity and specificity of the newly established panCoV RT-PCR assay had been 100%.

A one-step real-time RT-PCR helps to identify mixed rotavirus infections in Mexico.

400 avian fecal (RNA) samples had been additional examined concurrently for CoV by the brand new panCoV RT-PCR and a one-step RT-PCR assay with the δ-CoV nucleocapsid-specific common primers. 4 of 400 avian samples had been optimistic for CoV, three of which had been optimistic for δ-CoV by the traditional RT-PCR. PanCoV RT-PCR fragments for three of the four CoVs had been sequenced. Phylogenetic evaluation revealed the presence of 1 γ-CoV and two δ-CoV within the sequenced samples. The newly designed panCoV RT-PCR assay must be helpful for the detection of at present recognized CoVs in animal organic samples.

onestep multiplex real-time RTPCR for the common detection of all at present recognized CCHFV genotypes.

Crimean-Congo hemorrhagic fever (CCHF) is a deadly illness in people, which is endemic in lots of nations of Africa, Southern Asia and Southeastern Europe. It’s brought on by the Crimean-Congo hemorrhagic fever virus (CCHFV), which is an arthropod-borne virus (arbovirus) transmitted by ixodid ticks, primarily of the genus Hyalomma. Animals like hares, hedgehogs, cattle, camels and small ruminants can develop into contaminated with out growing scientific indicators. Seroconversion happens after a brief viremia of as much as two weeks, and thus seroprevalence research in ruminants can be utilized to disclose threat areas for the human inhabitants.

Virus detection by real-time quantitative reverse transcription polymerase chain response (RT-qPCR) is crucial to show an precise circulation of CCHFV in a rustic and can be used as diagnostic technique for acute human CCHFV infections. On this research, a brand new common one-step multiplex real-time RT-qPCR for the delicate and particular detection of CCHFV is introduced. For this goal, 14 new primers and a pair of probes had been concurrently used to detect RNAs representing all six CCHFV genotypes.

dsGreen for Real-Time PCR, 100×, 100 uL

11010 100 uL
EUR 59

dsGreen for Real-Time PCR, 100×, 2 mL

61010 2 mL
EUR 363

dsGreen for Real-Time PCR, 100×, 10 mL

81010 10 mL
EUR 869

dsGreen for Real-Time PCR, 100×, 50 mL

91010 50 mL
EUR 3169

dsGreen for Real-Time PCR, 100×, 1 mL

41010 1 mL
EUR 249

dsGreen for Real-Time PCR, 100×, 1.5 mL

51010 1.5 mL
EUR 331

dsGreen for Real-Time PCR, 100×, 5 mL

71010 5 mL
EUR 625

hsa-let-7a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-let-7b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-let-7c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-let-7d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-let-7e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-let-7g Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-let-7i Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-mir-1 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097612
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hsa-mir-7 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097613
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hsa-mir-9 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-mir-9* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097615
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hsa-mir-10a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097616
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hsa-mir-10b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097617
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hsa-mir-10a* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-mir-15a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097619
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hsa-mir-15b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-mir-16 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097622
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hsa-mir-18a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097625
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hsa-mir-18b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097626
  • EUR 732.00
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hsa-mir-19a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097627
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hsa-mir-19b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097628
  • EUR 732.00
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hsa-mir-20a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097629
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hsa-mir-20b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097630
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hsa-mir-21 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097631
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hsa-mir-22 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097632
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hsa-mir-23a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097633
  • EUR 732.00
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  • 100 rxns
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hsa-mir-23b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097634
  • EUR 732.00
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  • 100 rxns
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hsa-mir-25 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097636
  • EUR 732.00
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  • EUR 523.00
  • 100 rxns
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hsa-mir-26a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097637
  • EUR 732.00
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  • 100 rxns
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hsa-mir-26b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097638
  • EUR 732.00
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  • EUR 523.00
  • 100 rxns
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hsa-mir-27a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097639
  • EUR 732.00
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  • 100 rxns
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hsa-mir-27b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097640
  • EUR 732.00
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  • 100 rxns
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hsa-mir-29a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097642
  • EUR 732.00
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hsa-mir-29b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097643
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hsa-mir-29c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097644
  • EUR 732.00
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hsa-mir-30a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097645
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hsa-mir-30b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097647
  • EUR 732.00
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hsa-mir-30c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097648
  • EUR 732.00
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hsa-mir-30d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097650
  • EUR 732.00
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  • 100 rxns
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  • 50 rxns

hsa-mir-30e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097651
  • EUR 732.00
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  • 100 rxns
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hsa-mir-31 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097652
  • EUR 732.00
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hsa-mir-32 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-mir-33a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-mir-34a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-mir-34b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-mir-92a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-mir-92b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-mir-93 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-mir-95 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-mir-96 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-mir-98 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-mir-99a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-mir-99b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-mir-100 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-mir-101 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

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hsa-mir-103 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097675
  • EUR 732.00
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hsa-mir-105 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097676
  • EUR 732.00
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  • EUR 523.00
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hsa-mir-106a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097677
  • EUR 732.00
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  • EUR 523.00
  • 100 rxns
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  • 50 rxns

hsa-mir-106b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097678
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
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  • 50 rxns

hsa-mir-107 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097679
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
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  • 50 rxns

hsa-mir-124 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097680
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-125a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097681
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-127 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097686
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-129 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097689
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-130a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097690
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-130b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097691
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-132 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097692
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-133a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097693
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-133b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097694
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-134 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097695
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-135b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097696
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-137 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097699
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-138 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097700
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-139 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097701
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-140 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097702
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-141 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097703
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-143 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097706
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-145 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097708
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-146a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097709
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-149 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097711
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-150 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097712
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-151 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097714
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-152 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097715
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

Moreover, a GC-mirrored sequence inside the artificial RNAs allows the discrimination between true optimistic samples and unintentional laboratory contaminations. CCHFV unfavorable samples from completely different animal species and ten completely different members of the order Bunyavirales had been finally examined to disclose the specificity of the brand new RT-qPCR.

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