Speedy and Excessive-Throughput Reverse Transcriptase Quantitative PCR (RT-qPCR) Assay for Identification and Differentiation between SARS-CoV-2 Variants
- We present that the strain-specific reactions goal mutations which can be strongly related to the goal variants and never with different main recognized variants. The assay’s specificity was examined in opposition to a panel of respiratory pathogens (n = 16), displaying excessive specificity towards SC-2 RNA.
- The assay’s sensitivity was assessed utilizing each in vitro transcribed RNA and medical samples and was decided to be between 20 and 40 viral RNA copies per response. The assay efficiency was corroborated with Sanger and whole-genome sequencing, displaying full settlement with the sequencing outcomes.
- The brand new assay is at present applied within the routine diagnostic work on the Central Virology Laboratory, and could also be utilized in different laboratories to facilitate the analysis of those main worldwide-circulating SC-2 variants.
Validation of appropriate reference genes for quantitative expression evaluation by qPCR in bovine terminal ileum and ileocecal valve

A speedy and low-cost protocol for the detection of B.1.1.7 lineage of SARS-CoV-2 by utilizing SYBR Inexperienced-based RT-qPCR
Goat anti Mouse IgG1 (Alexa Fluor 488) |
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43R-1649 | Fitzgerald | 500 ug | EUR 684 |
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488) |
Anti-Hu CD16 Alexa Fluor® 488 |
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A4-646-T100 | ExBio | 100 tests | EUR 322.8 |
anti-PD-L1 VHH antibody (Alexa Fluor® 488) |
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JOT0002-5-2 | Jotbody | each | Ask for price |
AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate] |
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16891 | AAT Bioquest | 1 mg | EUR 211.2 |
AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin] |
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23153 | AAT Bioquest | 300 Tests | EUR 367.2 |
Anti-Hu CD72 Alexa Fluor® 488 |
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A4-310-T100 | ExBio | 100 tests | EUR 322.8 |
Anti-Bov CD9 Alexa Fluor® 488 |
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A4-354-C100 | ExBio | 0.1 mg | EUR 322.8 |
XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine* |
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4600 | AAT Bioquest | 1 mg | EUR 157 |
XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde* |
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9015 | AAT Bioquest | 5 mg | EUR 425 |
XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde* |
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9016 | AAT Bioquest | 1 mg | EUR 308 |
iFluor® 488 Styramide *Superior Replacement for Alexa Fluor 488 tyramide and Opal 520* |
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45020 | AAT Bioquest | 100 Slides | EUR 334 |
Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor® 488) |
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M09406 | BosterBio | 100ug | EUR 678 |
Description: Mouse Monoclonal LAMP3 (human) Antibody (104G4) (Alexa Fluor® 488). Validated in IHC and tested in Human. |
GPR12 Antibody |
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44910-100ul | SAB | 100ul | EUR 302.4 |
GPR12 Antibody |
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44910-50ul | SAB | 50ul | EUR 224.4 |
GPR12 antibody |
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70R-49804 | Fitzgerald | 100 ul | EUR 292.8 |
Description: Purified Polyclonal GPR12 antibody |
GPR12 antibody |
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70R-30940 | Fitzgerald | 100 ug | EUR 392.4 |
Description: Rabbit polyclonal GPR12 antibody |
GPR12 antibody |
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20R-GR041 | Fitzgerald | 50 ug | EUR 787.2 |
Description: Rabbit polyclonal GPR12 antibody |
GPR12 Antibody |
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ABD2721 | Lifescience Market | 100 ug | EUR 525.6 |
GPR12 Antibody |
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DF2721 | Affbiotech | 200ul | EUR 420 |
GPR12 Antibody |
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1-CSB-PA008905 | Cusabio |
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Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/5000 |
GPR12 Antibody |
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1-CSB-PA009742LA01HU | Cusabio |
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Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
Anti-Hu CD3 zeta (pY153) Alexa Fluor® 488 |
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A4-686-C100 | ExBio | 0.1 mg | EUR 322.8 |
Anti-Hu CD3 zeta (pY72) Alexa Fluor® 488 |
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A4-712-C100 | ExBio | 0.1 mg | EUR 322.8 |
Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488 |
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A4-730-C100 | ExBio | 0.1 mg | EUR 322.8 |
Anti-Hu CD3 zeta (pY111) Alexa Fluor® 488 |
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A4-737-C100 | ExBio | 0.1 mg | EUR 322.8 |