Speedy and Excessive-Throughput Reverse Transcriptase Quantitative PCR (RT-qPCR) Assay for Identification and Differentiation between SARS-CoV-2 Variants
- We present that the strain-specific reactions goal mutations which can be strongly related to the goal variants and never with different main recognized variants. The assay’s specificity was examined in opposition to a panel of respiratory pathogens (n = 16), displaying excessive specificity towards SC-2 RNA.
- The assay’s sensitivity was assessed utilizing each in vitro transcribed RNA and medical samples and was decided to be between 20 and 40 viral RNA copies per response. The assay efficiency was corroborated with Sanger and whole-genome sequencing, displaying full settlement with the sequencing outcomes.
- The brand new assay is at present applied within the routine diagnostic work on the Central Virology Laboratory, and could also be utilized in different laboratories to facilitate the analysis of those main worldwide-circulating SC-2 variants.
Validation of appropriate reference genes for quantitative expression evaluation by qPCR in bovine terminal ileum and ileocecal valve

A speedy and low-cost protocol for the detection of B.1.1.7 lineage of SARS-CoV-2 by utilizing SYBR Inexperienced-based RT-qPCR
Anti-RPSA Alexa Fluor® 488 |
|||
A4-829-C100 | ExBio | 0.1 mg | EUR 372 |
Alexa Fluor® 488 Streptavidin |
|||
E16FXP040G | EnoGene | 100 μg | EUR 758.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-rat CD3 |
|||
E16FRG003-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-rat CD4 |
|||
E16FRG004-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-rat CD25 |
|||
E16FRG025-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-rat CD45 |
|||
E16FRG045-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 rat IgG2a |
|||
E16FRCG002-100U | EnoGene | 100 μg | EUR 628.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-mouse IgD |
|||
E16SDG501-025U | EnoGene | 25 μg | EUR 411.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-mouse IgD |
|||
E16SDG501-100U | EnoGene | 100 μg | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-mouse IgE |
|||
E16SEG501-025U | EnoGene | 25 μg | EUR 325 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-mouse IgE |
|||
E16SEG501-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-mouse IgM |
|||
E16SMG501-025U | EnoGene | 25 μg | EUR 325 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-mouse IgM |
|||
E16SMG501-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-mouse CD3 |
|||
E16FMG003-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-mouse CD3 |
|||
E16FMG003-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-mouse CD4 |
|||
E16FMG004-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-mouse CD5 |
|||
E16FMG005-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-human CD3 |
|||
E16FHG003-025 | EnoGene | 25 tests | EUR 288.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-human CD3 |
|||
E16FHG003-050 | EnoGene | 50 tests | EUR 411.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-human CD3 |
|||
E16FHG003-100 | EnoGene | 100 Tests | EUR 682.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-human CD4 |
|||
E16FHG004-050 | EnoGene | 25 tests | EUR 343 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-human CD4 |
|||
E16FHG004-100 | EnoGene | 100 tests | EUR 457.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-human CD8 |
|||
E16FHG008-025 | EnoGene | 25 tests | EUR 288.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-human CD8 |
|||
E16FHG008-050 | EnoGene | 50 tests | EUR 411.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 488 anti-human CD8 |
|||
E16FHG008-100 | EnoGene | 100 tests | EUR 682.67 |
Description: Available in various conjugation types. |