Detection of six soil-transmitted helminths in human stool by qPCR– a scientific workflow
- Samples had been washed to take away potassium dichromate and DNA was extracted with the Mini-Beadbeater-24 and a column-based package. An SYBR inexperienced qPCR to detect the vertebrate mitochondrial gene was used as a DNA extraction management.
- Samples had been examined utilizing a probe-based multiplex qPCR concentrating on A. lumbricoides, T. trichiura and S. stercoralis, and in a second multiplex response to detect hookworms to the species stage (A. duodenale, A. ceylanicum, N. americanus).
- An inside amplification management in each multiplex assays was included to stop false-negative outcomes as a result of PCR inhibitors. Samples had been homogenised for a single cycle of 40 seconds to launch STH DNA and washed stool was saved for as much as 15 weeks at -30°C with out compromising DNA.
- Our multiplex qPCR detected a number of species of STH with out lowered sensitivity in comparison with singleplex. qPCR knowledge from 40 stools was validated towards STH-positive stools decided by microscopy.
- We have now developed and validated an environment friendly and staged system for detecting six clinically vital STH affecting people that may very well be simply carried out with out superior automation in any qPCR-capable laboratory.

Comparability of the Fast Antigen Testing Methodology With RT-qPCR for the Analysis of COVID-19
Utilizing qPCR and high-resolution sensor knowledge to mannequin a multi-species Pseudo-nitzschia (Bacillariophyceae) bloom in southeastern Australia
![]() Parotid Dissociation System 2 (Parotid), Mouse and Rat |
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4-20392 | CHI Scientific | ea | Ask for price |
![]() Total Protein from Human Adult Normal Tissue: Salivary Gland |
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P1234212 | Biochain | 1 mg | EUR 356 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
![]() Membrane Protein from Human Adult Normal Tissue: Salivary Gland |
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P3234212 | Biochain | 0.1 mg | EUR 516 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
![]() Total RNA from Human Adult Normal Tissue: Salivary Gland |
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R1234212-10 | Biochain | 10 ug | EUR 194 |
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() Human Salivary Gland Tissue Slide (Abnormal) (5 slides/pk) |
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HTS-12128 | Alpha Diagnostics | 1 pk | EUR 286 |
![]() cDNA from Monkey (Cynomolgus) Normal Tissue: Brain: Pineal Gland |
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C1534067-Cy | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() Parotid Dissociation System 4 (Parotid acinar), Mouse and Rat |
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4-20394 | CHI Scientific | ea | Ask for price |
![]() Aedes aegypti 37KDA salivary gland allergen Aed a 2 (D7) |
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1-CSB-YP321587AXQ | Cusabio |
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Description: Recombinant Aedes aegypti 37KDA salivary gland allergen Aed a 2(D7) expressed in Yeast |
![]() Aedes aegypti 37KDA salivary gland allergen Aed a 2 (D7) |
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1-CSB-EP321587AXQ | Cusabio |
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Description: Recombinant Aedes aegypti 37KDA salivary gland allergen Aed a 2(D7) expressed in E.coli |
![]() Human Salivary Gland Tissue Slide (Mucoepidermoid carcinoma) (5 slides/pk) |
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HTS-12109 | Alpha Diagnostics | 1 pk | EUR 286 |
![]() anti-parotid duct antibody/ Rat anti- parotid duct antibody ELIS |
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ELA-E0500r | Lifescience Market | 96 Tests | EUR 886 |
![]() Parotid Dissociation System 3 (Acinar, Exorbital Iacrimal, parotid), Mouse and Rat |
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4-20393 | CHI Scientific | ea | Ask for price |
![]() Pineal Gland |
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PC35143 | Neuromics | Pineal Gland | EUR 396 |
![]() Parotid secretory protein Antibody |
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abx431530-200ul | Abbexa | 200 ul | EUR 384 |
![]() Parotid secretory protein Antibody |
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abx431531-200ul | Abbexa | 200 ul | EUR 286 |
![]() Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol) |
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RAT-5 | Alpha Diagnostics | 1 | EUR 1138 |
![]() Pancreas Dissociation System 6 (Acinar, rat parotid), Rat |
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4-20376 | CHI Scientific | ea | Ask for price |
![]() Rat anti parotid duct antibody ELISA kit |
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E02A2104-192T | BlueGene | 192 tests | EUR 1270 |
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
![]() Rat anti parotid duct antibody ELISA kit |
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E02A2104-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
![]() Rat anti parotid duct antibody ELISA kit |
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E02A2104-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
![]() Rat Salivary Amylase ELISA kit |
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E02S0191-192T | BlueGene | 192 tests | EUR 1270 |
Description: A competitive ELISA for quantitative measurement of Rat Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
![]() Rat Salivary Amylase ELISA kit |
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E02S0191-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
Description: A competitive ELISA for quantitative measurement of Rat Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
![]() Rat Salivary Amylase ELISA kit |
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E02S0191-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
Description: A competitive ELISA for quantitative measurement of Rat Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
![]() Human Salivary IGF(salivary IGF) ELISA Kit |
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QY-E05383 | Qayee Biotechnology | 96T | EUR 361 |
![]() Parotid Dissociation System 1 (Epithelial), Mouse and Rat |
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4-20391 | CHI Scientific | ea | Ask for price |
![]() Rat Salivary duct autoantibody ELISA kit |
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E02S0244-192T | BlueGene | 192 tests | EUR 1270 |
Description: A sandwich ELISA for quantitative measurement of Rat Salivary duct autoantibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
![]() Rat Salivary duct autoantibody ELISA kit |
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E02S0244-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
Description: A sandwich ELISA for quantitative measurement of Rat Salivary duct autoantibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
![]() Rat Salivary duct autoantibody ELISA kit |
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E02S0244-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
Description: A sandwich ELISA for quantitative measurement of Rat Salivary duct autoantibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
![]() Frozen Tissue Section - Human Tumor: Parotid |
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T1235190 | Biochain | 5 slides | EUR 338 |
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
![]() Anti-Parotid secretory protein (mouse) antibody |
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STJ72465 | St John's Laboratory | 100 µg | EUR 260 |
![]() cDNA from Rat Normal Tissue: Adipose |
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C1434003 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Rat Normal Tissue: Bladder |
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C1434010 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Rat Normal Tissue: Brain |
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C1434035 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Rat Normal Tissue: Colon |
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C1434090 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Rat Normal Tissue: Heart |
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C1434122 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Rat Normal Tissue: Kidney |
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C1434142 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Rat Normal Tissue: Liver |
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C1434149 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Rat Normal Tissue: Lung |
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C1434152 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |