Detection of six soil-transmitted helminths in human stool by qPCR– a scientific workflow
- Samples had been washed to take away potassium dichromate and DNA was extracted with the Mini-Beadbeater-24 and a column-based package. An SYBR inexperienced qPCR to detect the vertebrate mitochondrial gene was used as a DNA extraction management.
- Samples had been examined utilizing a probe-based multiplex qPCR concentrating on A. lumbricoides, T. trichiura and S. stercoralis, and in a second multiplex response to detect hookworms to the species stage (A. duodenale, A. ceylanicum, N. americanus).
- An inside amplification management in each multiplex assays was included to stop false-negative outcomes as a result of PCR inhibitors. Samples had been homogenised for a single cycle of 40 seconds to launch STH DNA and washed stool was saved for as much as 15 weeks at -30°C with out compromising DNA.
- Our multiplex qPCR detected a number of species of STH with out lowered sensitivity in comparison with singleplex. qPCR knowledge from 40 stools was validated towards STH-positive stools decided by microscopy.
- We have now developed and validated an environment friendly and staged system for detecting six clinically vital STH affecting people that may very well be simply carried out with out superior automation in any qPCR-capable laboratory.

Comparability of the Fast Antigen Testing Methodology With RT-qPCR for the Analysis of COVID-19
Utilizing qPCR and high-resolution sensor knowledge to mannequin a multi-species Pseudo-nitzschia (Bacillariophyceae) bloom in southeastern Australia
Salivary Gland Tissue Slide (Abnormal) |
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12-128-4um | ProSci | 4 um | EUR 216.6 |
cDNA from Monkey (Rhesus) Normal Tissue: Parotid |
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C1534190 | Biochain | 40 reactions | EUR 648 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Monkey (Cynomolgus) Normal Tissue: Parotid |
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C1534190-Cy | Biochain | 40 reactions | EUR 648 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Human Adult Salivary (Normal) Gland Whole tissue lysate |
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HAL-20103 | Alpha Diagnostics | 1 mg | EUR 927.6 |
Total Protein from Human Adult Normal Tissue: Salivary Gland |
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P1234212 | Biochain | 1 mg | EUR 427.2 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Membrane Protein from Human Adult Normal Tissue: Salivary Gland |
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P3234212 | Biochain | 0.1 mg | EUR 619.2 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Human Salivary Gland Tissue Slide (Abnormal) (5 slides/pk) |
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HTS-12128 | Alpha Diagnostics | 1 pk | EUR 343.2 |
Total RNA from Human Adult Normal Tissue: Salivary Gland |
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R1234212-10 | Biochain | 10 ug | EUR 232.8 |
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Parotid Dissociation System 2 (Parotid), Mouse and Rat |
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4-20392 | CHI Scientific | ea | Ask for price |
cDNA from Monkey (Cynomolgus) Normal Tissue: Brain: Pineal Gland |
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C1534067-Cy | Biochain | 40 reactions | EUR 648 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Parotid Dissociation System 4 (Parotid acinar), Mouse and Rat |
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4-20394 | CHI Scientific | ea | Ask for price |
Aedes aegypti 37KDA salivary gland allergen Aed a 2 (D7) |
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1-CSB-YP321587AXQ | Cusabio |
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Description: Recombinant Aedes aegypti 37KDA salivary gland allergen Aed a 2(D7) expressed in Yeast |
Aedes aegypti 37KDA salivary gland allergen Aed a 2 (D7) |
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1-CSB-EP321587AXQ | Cusabio |
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Description: Recombinant Aedes aegypti 37KDA salivary gland allergen Aed a 2(D7) expressed in E.coli |
Human Salivary Gland Tissue Slide (Mucoepidermoid carcinoma) (5 slides/pk) |
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HTS-12109 | Alpha Diagnostics | 1 pk | EUR 343.2 |
anti-parotid duct antibody/ Rat anti- parotid duct antibody ELIS |
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ELA-E0500r | Lifescience Market | 96 Tests | EUR 1063.2 |
Parotid Dissociation System 3 (Acinar, Exorbital Iacrimal, parotid), Mouse and Rat |
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4-20393 | CHI Scientific | ea | Ask for price |
Pineal Gland |
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PC35143 | Neuromics | Pineal Gland | EUR 475.2 |
Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol) |
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RAT-5 | Alpha Diagnostics | 1 | EUR 1365.6 |
Parotid secretory protein Antibody |
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abx431530-200ul | Abbexa | 200 ul | EUR 460.8 |
Parotid secretory protein Antibody |
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abx431531-200ul | Abbexa | 200 ul | EUR 343.2 |
Parotid Membrane Tumor Lysate |
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XBL-10807 | ProSci | 0.1 mg | EUR 1023 |
Description: Human parotid tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human parotid tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated parotid tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated parotid tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
Pancreas Dissociation System 6 (Acinar, rat parotid), Rat |
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4-20376 | CHI Scientific | ea | Ask for price |
Rat anti parotid duct antibody ELISA kit |
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E02A2104-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat anti parotid duct antibody ELISA kit |
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E02A2104-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat anti parotid duct antibody ELISA kit |
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E02A2104-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Salivary Amylase ELISA kit |
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E02S0191-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Rat Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Salivary Amylase ELISA kit |
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E02S0191-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Rat Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Salivary Amylase ELISA kit |
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E02S0191-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Rat Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Salivary IGF(salivary IGF) ELISA Kit |
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QY-E05383 | Qayee Biotechnology | 96T | EUR 433.2 |
Parotid Dissociation System 1 (Epithelial), Mouse and Rat |
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4-20391 | CHI Scientific | ea | Ask for price |
Rat Salivary duct autoantibody ELISA kit |
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E02S0244-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Rat Salivary duct autoantibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Salivary duct autoantibody ELISA kit |
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E02S0244-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Rat Salivary duct autoantibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Salivary duct autoantibody ELISA kit |
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E02S0244-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Rat Salivary duct autoantibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Anti-Parotid secretory protein (mouse) antibody |
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STJ72465 | St John's Laboratory | 100 µg | EUR 312 |
Frozen Tissue Section - Human Tumor: Parotid |
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T1235190 | Biochain | 5 slides | EUR 405.6 |
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
cDNA from Rat Normal Tissue: Adipose |
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C1434003 | Biochain | 40 reactions | EUR 648 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Rat Normal Tissue: Bladder |
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C1434010 | Biochain | 40 reactions | EUR 451.2 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Rat Normal Tissue: Brain |
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C1434035 | Biochain | 40 reactions | EUR 451.2 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |