Background: Diarrheal illness is a number one reason behind morbidity and mortality globally, particularly in low- and middle-income nations. Excessive-throughput and low-cost approaches to determine etiologic brokers are wanted to information public well being mitigation. Nanoliter-qPCR (nl-qPCR) is a lovely different to costlier strategies but is nascent in software and and not using a proof-of-concept amongst hospitalized sufferers.
Strategies: A census-based research was performed amongst diarrheal sufferers admitted at two authorities hospitals in rural Bangladesh throughout a diarrheal outbreak interval. DNA was extracted from stool samples and assayed by nl-qPCR for frequent bacterial, protozoan, and helminth enteropathogens as the first final result.
Outcomes: A complete of 961 sufferers had been enrolled; stool samples had been collected from 827 sufferers. Enteropathogens had been detected in 69% of affected person samples; A couple of enteropathogen was detected in 32%. Enteropathogens mostly detected had been enteroaggregative Escherichia coli (26.0%), Shiga toxin-producing E.coli (18.3%), enterotoxigenic E. coli (15.5% warmth secure toxin optimistic, 2.2% warmth labile toxin optimistic), Shigella spp. (14.8%), and Vibrio cholerae (9.0%). Geospatial evaluation revealed that the median variety of pathogens per affected person and the proportion of instances presenting with extreme dehydration had been best amongst sufferers residing closest to the research hospitals.”
Conclusions: This research demonstrates a proof-of-concept for nl-qPCR as a high-throughput low-cost technique for enteropathogen detection amongst hospitalized sufferers.
Detection of six soil-transmitted helminths in human stool by qPCR– a scientific workflow
Soil-transmitted helminths (STH) infect as much as one-quarter of the worldwide inhabitants, with a major related illness burden. The primary human STH are: Ancylostoma spp. and Necator americanus (hookworms); Ascaris lumbricoides, Trichuris trichiura, and Strongyloides stercoralis. The intention of this research was to ascertain a scalable system for stool STH multiplex quantitative real-time polymerase chain reactions (qPCR). Stool samples collected in Fiji and preserved in potassium dichromate had been transferred to Melbourne at ambient temperature.
- Samples had been washed to take away potassium dichromate and DNA was extracted with the Mini-Beadbeater-24 and a column-based package. An SYBR inexperienced qPCR to detect the vertebrate mitochondrial gene was used as a DNA extraction management.
- Samples had been examined utilizing a probe-based multiplex qPCR concentrating on A. lumbricoides, T. trichiura and S. stercoralis, and in a second multiplex response to detect hookworms to the species stage (A. duodenale, A. ceylanicum, N. americanus).
- An inside amplification management in each multiplex assays was included to stop false-negative outcomes as a result of PCR inhibitors. Samples had been homogenised for a single cycle of 40 seconds to launch STH DNA and washed stool was saved for as much as 15 weeks at -30°C with out compromising DNA.
- Our multiplex qPCR detected a number of species of STH with out lowered sensitivity in comparison with singleplex. qPCR knowledge from 40 stools was validated towards STH-positive stools decided by microscopy.
- We have now developed and validated an environment friendly and staged system for detecting six clinically vital STH affecting people that may very well be simply carried out with out superior automation in any qPCR-capable laboratory.
Comparability of the Fast Antigen Testing Methodology With RT-qPCR for the Analysis of COVID-19
Background: Coronavirus illness 2019 (COVID-19) has until now affected about 110 million individuals globally. It has not spared any nation and has led to 24 lakh deaths. Because of this, the testing needed to be elevated manifold resulting in depletion within the variety of the quantitative reverse transcription polymerase chain response (RT-qPCR) kits. Level-of-care fast antigen-based assessments had been developed as a way to meet the growing calls for. The target of this research was to match the efficiency of a fast chromatographic check (index check) with a gold customary check (RT-qPCR).
Strategies: A retrospective evaluation was accomplished at a tertiary care instructing hospital in Jap Uttar Pradesh, India. Paired samples had been taken from all sufferers reporting to the clinic for antigen-based fast diagnostic testing (RDT) and RT-qPCR. The sensitivity and specificity had been calculated to guage the efficiency of the RDT.
Outcomes: The general sensitivity and specificity of the RDT had been noticed to be 53.6% (39.7-67.0) and 97.35% (94.6-98.9), respectively. In symptomatic people, the sensitivity was larger 61.0% (44.5-75.8). The check positivity charges of RDT had been discovered to be larger at a cycle threshold worth ≤20.
Conclusion: RDT can be utilized as a screening check to rule within the an infection particularly in symptomatic sufferers who’re extra vulnerable to unfold the illness. It is a crucial weapon within the armamentarium of public healthcare for the containment of COVID-19.
Key phrases: covid-19; immunochromatography; rdt; rt-qpcr; check traits.
Utilizing qPCR and high-resolution sensor knowledge to mannequin a multi-species Pseudo-nitzschia (Bacillariophyceae) bloom in southeastern Australia
Dangerous algal blooms, together with these brought on by the poisonous diatom Pseudo-nitzschia, can have vital impacts on human well being, ecosystem functioning and finally meals safety. Within the present research we characterised a bloom of species of Pseudo-nitzschia that occurred in a south-eastern Australian oyster-growing estuary in 2019. Utilizing mild microscopy, mixed with molecular (ITS/5.8S and LSU D1-D3 rDNA areas) and toxicological proof, we noticed the bloom to include a number of species of Pseudo-nitzschia together with P. cf. cuspidata, P. hasleana, P. fraudulenta and P. multiseries, with P. cf. cuspidata being the one species that produced domoic acid (3.1 pg DA per cell).
As a number of species of Pseudo-nitzschia co-occurred, solely one in every of which produced DA, we developed a fast, delicate and environment friendly quantitative real-time polymerase chain response (qPCR) assay to detect solely species belonging to the P. pseudodelicatissima complicated Clade I, to which P. cf. cuspidata belongs, and this indicated that P. cuspidata or intently associated strains could have dominated the Pseudo-nitzschia group right now. Lastly, utilizing excessive decision water temperature and salinity sensor knowledge, we modeled the connection between mild microscopy decided abundance of P. delicatissima group and environmental variables (temperature, salinity, rainfall) at two websites throughout the estuary.
A complete of eight Common Linear Fashions (GLMs) explaining between 9 and 54% of the deviance recommended that the temperature (growing) and/or salinity (lowering) knowledge had been typically extra predictive of excessive cell concentrations than the rainfall knowledge at each websites, and that total, cell concentrations had been extra predictive on the extra oceanic website than the extra upstream website, utilizing this technique.
We conclude that the mix of fast molecular strategies corresponding to qPCR and real-time sensor knowledge modeling, can present a extra fast and efficient early warning of dangerous algal blooms of species of Pseudo-nitzschia, leading to extra helpful regulatory and administration outcomes.
, Mouse and Rat) Parotid Dissociation System 2 (Parotid), Mouse and Rat |
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| 4-20392 | CHI Scientific | ea | Ask for price |
 Total Protein from Human Adult Normal Tissue: Salivary Gland |
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| P1234212 | Biochain | 1 mg | EUR 356 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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 Membrane Protein from Human Adult Normal Tissue: Salivary Gland |
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| P3234212 | Biochain | 0.1 mg | EUR 516 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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 Total RNA from Human Adult Normal Tissue: Salivary Gland |
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| R1234212-10 | Biochain | 10 ug | EUR 194 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 (5 slides/pk)) Human Salivary Gland Tissue Slide (Abnormal) (5 slides/pk) |
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| HTS-12128 | Alpha Diagnostics | 1 pk | EUR 286 |
 Normal Tissue: Brain: Pineal Gland) cDNA from Monkey (Cynomolgus) Normal Tissue: Brain: Pineal Gland |
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| C1534067-Cy | Biochain | 40 reactions | EUR 540 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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, Mouse and Rat) Parotid Dissociation System 4 (Parotid acinar), Mouse and Rat |
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| 4-20394 | CHI Scientific | ea | Ask for price |
) Aedes aegypti 37KDA salivary gland allergen Aed a 2 (D7) |
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| 1-CSB-YP321587AXQ | Cusabio |
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Description: Recombinant Aedes aegypti 37KDA salivary gland allergen Aed a 2(D7) expressed in Yeast |
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Aedes aegypti 37KDA salivary gland allergen Aed a 2 (D7) |
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| 1-CSB-EP321587AXQ | Cusabio |
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Description: Recombinant Aedes aegypti 37KDA salivary gland allergen Aed a 2(D7) expressed in E.coli |
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 (5 slides/pk)) Human Salivary Gland Tissue Slide (Mucoepidermoid carcinoma) (5 slides/pk) |
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| HTS-12109 | Alpha Diagnostics | 1 pk | EUR 286 |
 anti-parotid duct antibody/ Rat anti- parotid duct antibody ELIS |
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| ELA-E0500r | Lifescience Market | 96 Tests | EUR 886 |
, Mouse and Rat) Parotid Dissociation System 3 (Acinar, Exorbital Iacrimal, parotid), Mouse and Rat |
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| 4-20393 | CHI Scientific | ea | Ask for price |
 Pineal Gland |
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| PC35143 | Neuromics | Pineal Gland | EUR 396 |
 Parotid secretory protein Antibody |
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| abx431530-200ul | Abbexa | 200 ul | EUR 384 |
Parotid secretory protein Antibody |
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| abx431531-200ul | Abbexa | 200 ul | EUR 286 |
) Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol) |
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| RAT-5 | Alpha Diagnostics | 1 | EUR 1138 |
, Rat) Pancreas Dissociation System 6 (Acinar, rat parotid), Rat |
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| 4-20376 | CHI Scientific | ea | Ask for price |
 Rat anti parotid duct antibody ELISA kit |
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| E02A2104-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat anti parotid duct antibody ELISA kit |
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| E02A2104-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat anti parotid duct antibody ELISA kit |
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| E02A2104-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 Rat Salivary Amylase ELISA kit |
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| E02S0191-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Rat Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Salivary Amylase ELISA kit |
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| E02S0191-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Rat Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Salivary Amylase ELISA kit |
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| E02S0191-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Rat Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 ELISA Kit) Human Salivary IGF(salivary IGF) ELISA Kit |
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| QY-E05383 | Qayee Biotechnology | 96T | EUR 361 |
, Mouse and Rat) Parotid Dissociation System 1 (Epithelial), Mouse and Rat |
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| 4-20391 | CHI Scientific | ea | Ask for price |
 Rat Salivary duct autoantibody ELISA kit |
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| E02S0244-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A sandwich ELISA for quantitative measurement of Rat Salivary duct autoantibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Salivary duct autoantibody ELISA kit |
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| E02S0244-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A sandwich ELISA for quantitative measurement of Rat Salivary duct autoantibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Salivary duct autoantibody ELISA kit |
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| E02S0244-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A sandwich ELISA for quantitative measurement of Rat Salivary duct autoantibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 Frozen Tissue Section - Human Tumor: Parotid |
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| T1235190 | Biochain | 5 slides | EUR 338 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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 antibody) Anti-Parotid secretory protein (mouse) antibody |
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| STJ72465 | St John's Laboratory | 100 µg | EUR 260 |
 cDNA from Rat Normal Tissue: Adipose |
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| C1434003 | Biochain | 40 reactions | EUR 540 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 cDNA from Rat Normal Tissue: Bladder |
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| C1434010 | Biochain | 40 reactions | EUR 376 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 cDNA from Rat Normal Tissue: Brain |
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| C1434035 | Biochain | 40 reactions | EUR 376 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 cDNA from Rat Normal Tissue: Colon |
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| C1434090 | Biochain | 40 reactions | EUR 540 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 cDNA from Rat Normal Tissue: Heart |
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| C1434122 | Biochain | 40 reactions | EUR 376 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 cDNA from Rat Normal Tissue: Kidney |
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| C1434142 | Biochain | 40 reactions | EUR 376 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 cDNA from Rat Normal Tissue: Liver |
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| C1434149 | Biochain | 40 reactions | EUR 376 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 cDNA from Rat Normal Tissue: Lung |
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| C1434152 | Biochain | 40 reactions | EUR 376 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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