Exosome isolation kits
A high-efficiency exosome isolation kit can be of great help to all exosome researchers. Its high purity, high yield, and easy operation make it an excellent research tool.
As we know, exosomes are small membrane vesicles (30-150 nm) of endocytic origin, which are eliminated by all types of cells under normal and pathophysiological conditions. Exosomes have pleiotropic physiological and pathological functions and play an important role in various pathological conditions such as cancer, infectious and neurodegenerative diseases.
Exosomes have received significant attention for their role in pathobiological processes and are being explored as a tool for disease diagnosis and management. Consequently, various isolation methods based on different principles have been developed for the isolation of exosomes, such as differential ultracentrifugation, density gradient ultracentrifugation, ultracentrifugation, chromatography, magnetic bead immunoassay, polypolymer precipitation method, etc. irreplaceable.
CUSABIO Exosome Isolation Kits purify the exosome by affinity purification. High purity exosomes and other Evs (extracellular vesicles) from the cell culture medium and body fluid (high yield by normal microfiltration) can be easily obtained by this method.
- Suitable for many subsequent experiments
Experiments include transmission electron microscope analysis, nanoparticle tracking analysis, NanoFCM analysis, Western Blot, quantitative fluorescence (qPCR), high-throughput sequencing, etc.
- Suitable for a variety of sample types
The CUSABIO Exosome Isolation Kit provides a simple and reliable method to extract intact exosomes from cell culture supernatant, serum, plasma, latex, urine, and saliva.
- Can be used on the supernatant of a variety of cells
CUSABIO Exosome Isolation Kits are available to isolate exosomes from the supernatant of various cells, such as A375, HEPG2, PC-3, Hela, U87, MG63, etc.
- High purity
The exosomes extracted by the CUSABIO kits are complete, spherical or plate-shaped, with uniform particles. The vesicles are structurally intact. The following are part of the results.
- High performance
There are up to 3 x 1011 purified exosome particles per millilitre.
- High efficiency
The exosomes extracted by the CUSABIO Exosome Isolation Kit are superior to the other three well-known brand kits and comparable to ultracentrifugation.
- Easy to use
No ultracentrifugation or phenol/chloroform step is required. Does not require equipment. Just follow our protocol, you will get the exosomes soon.
- Competitive prices and excellent technical support.
The prices of the CUSABIO exosome isolation kits are very competitive. We have an efficient management and production system so that we can manufacture premium reagents at a lower cost and our clients can obtain better quotes.
How to preserve purified exosomal vesicles？
Store at 4 ℃ for short term storage or -80 ℃ for long term storage. It is recommended to store it after taking aliquots to avoid repeated freezing and thawing.
How to obtain serum culture cells without exosomes?
A. Ultracentrifuge the serum from the cell culture for 10 hours to remove the exosomes from the serum (aseptic operation).
B. Choose serum-free media for cell culture.
C. Choose serum without exosomes for cell culture.
How to identify exosomes extracted from cells?
WB validation of specific markers, transmission electron microscope (TEM), particle size (NanoFCM, NTA), etc.
What are the advantages of the CUSABIO Exosome Isolation Kit over ultracentrifugation?
A. Simple, stable and highly repeatable.
B. Does not require equipment.
What are exosomes used for?
Exosomes contain specific cell loads of nucleic acids, proteins, lipids, and metabolites, which facilitate intercellular communication that contributes to a spectrum of biological processes in health and disease. Exosomes are related to immune responses, viral pathogenicity, chronic inflammation, neurodegenerative and cardiovascular diseases, even cancer progression. In addition, exosomes are designed for therapeutic nanocarriers for the delivery of drugs and genes to the desired target, thus achieving the therapeutic control of many diseases.
How do exosomes work?
Upon binding of the exosome to the target cells through ligand-receptor interactions, the content of the exosomes is internalized into the recipient cells. These active biological molecules, such as proteins, lipids, and genetic materials, that mediate exosomal intercellular communication, ultimately reprogram the recipient cells.
Are exosomes good or bad?
Exosomes play a dual role in human health. They maintain cellular health by removing damaged and unwanted proteins and structures in cells. And they are also involved in immunity and reproduction in healthy individuals. However, cancer-derived exosomes participate in tumour growth, progression, spread, angiogenesis, and metastasis by transferring their charges of cytokines, mRNA, and miRNA to target cells. Resistance to cancer therapy is also attributed to exosomes of tumour origin. Furthermore, some exosomes are implicated in the pathogenesis of the disease, including neurodegenerative and cardiovascular diseases.
What is exosome therapy?
Exosome therapy is a form of regenerative treatment in which therapeutic payloads are delivered through exosomes to the affected site to achieve therapeutic effects. Therapeutic exosomes are administered by injection or infusion at the treatment site, where they target and release their content of endogenous and modified bioactive molecules, such as miRNAs and proteins, into damaged cells or tissues, stimulating faster healing and decreasing inflammation.
Are exosomes better than stem cells?
Compared to stem cells, exosomes are simpler, safer, cheaper, and easier to store and transport. In terms of therapeutic effects, the exosome shows very low immunogenicity and does not trigger complications. Furthermore, exosomes cannot replicate, do not deteriorate, and are not infected by viruses.