Development of a New Internally Controlled One-Step Real-Time RT-PCR for the Molecular Detection of Enterovirus A71 in Africa and Madagascar

Development of a New Internally Controlled One-Step Real-Time RT-PCR for the Molecular Detection of Enterovirus A71 in Africa and Madagascar

Enterovirus A71 (EV-A71) is a number one reason behind hand-foot-and-mouth illness (HFMD) and might be related to extreme neurological problems. EV-A71 strains might be labeled into seven genogroups, A-H, on the idea of the VP1 capsid protein gene sequence. Genogroup A contains the prototype pressure; genogroups B and C are accountable of main outbreaks worldwide, however little is understood in regards to the others, notably genogroups E and F, which have been not too long ago recognized in Africa and Madagascar, respectively. The circulation of EV-A71 within the African area is poorly recognized and possibly underestimated. A speedy and particular assay for detecting all genogroups of EV-A71 is required.

On this examine, we developed a real-time RT-PCR assay with a aggressive inner management (IC). The primers and TaqMan probe particularly goal the genomic area encoding the VP1 capsid protein. Various EV-A71 RNAs have been efficiently amplified from the genogroups A, B, C, D, E, and F, with related sensitivity and strong reproducibility. Neither cross response with different EVs nor main interference with the aggressive IC was detected. Experimentally spiked stool and plasma specimens offered constant and reproducible outcomes, and validated the usefulness of the IC for demonstrating the presence of PCR inhibitors in samples.

The evaluation in an African laboratories community of 1889 untyped enterovirus isolates detected 15 EV-A71 of various genogroups. This particular real-time RT-PCR assay supplies a strong and delicate methodology for the detection of EV-A71 in organic specimens and for the epidemiological monitoring of EV-A71 together with its not too long ago found genogroups.

Formalin-fixed paraffin-embedded (FFPE) tissues are an vital supply for investigation of dengue virus (DENV) an infection, notably when blood or recent frozen (FF) samples are unavailable. Histopathologic options and immunohistochemistry could have poor sensitivity and serotype willpower is just not at all times doable. Viral RNA genome detection assessments are sooner and regarded probably the most delicate approach for this sort of evaluation, nonetheless, the usage of molecular strategies utilized to FFPE tissues remains to be restricted.

The authors utilized a single-step multiplex reverse transcription-quantitative polymerase chain response (RT-qPCR) for the investigation of DENV an infection and typing to FFPE samples of 32 deadly circumstances acquired throughout the 2019 outbreak that occurred in São Paulo state, Brazil. The authors in contrast the outcomes with these obtained utilizing FF tissues. Of the 24 circumstances with each FF and FFPE samples, 22 (91.67%) of the FF and 19 (76.20%) of the FFPE specimens have been optimistic. Two circumstances (8.33%) examined unfavourable in each forms of samples.

onestepone-tube real-time RTPCR primarily based assay with an automatic evaluation for detection of SARS-CoV-2

Early analysis of SARS-CoV-2 contaminated sufferers is important to regulate the dynamics of the COVID-19 pandemic. We develop a speedy and correct one-step multiplex TaqMan probe-based real-time RT-PCR assay, together with a computational software to systematically analyse the info. Our assay may detect to a restrict of 15 copies of SARS-CoV-2 transcripts-based on experiments carried out by spiking whole human RNA with in vitro synthesized viral transcripts. The assay was evaluated by performing 184 validations for the SARS-CoV-2 Nucleocapsid gene and human RNase P as an inner management reference gene with dilutions starting from 1-100 ng for human RNA on a cohort of 26 medical samples. 5 of 26 sufferers have been confirmed to be contaminated with SARS-CoV-2, whereas 21 examined unfavourable, in keeping with the requirements.

The accuracy of the assay was discovered to be 100% delicate and 100% particular primarily based on the 26 medical samples that must be additional verified utilizing a lot of medical samples. In abstract, we current a speedy, simple to implement real-time PCR primarily based assay with automated evaluation utilizing a novel COVID qPCR Analyzer software with graphical person interface (GUI) to research the uncooked qRT-PCR information in an unbiased method at a value of underneath $Three per response and turnaround time of lower than 2h, to allow in-house SARS-CoV-2 testing throughout laboratories.

Development of a New Internally Controlled One-Step Real-Time RT-PCR for the Molecular Detection of Enterovirus A71 in Africa and Madagascar

The nucleotide area within the ORF1ab gene that has the best variations between the human coronavirus and the bat coronavirus was chosen. Primers have been designed after that sequence. All diagnostic primers are species-specific because the 3´ finish of the sequence differs from that of different species. A primer set additionally creates an artificial optimistic management. Amplified merchandise have been seen in a 2.5% agarose gel, in addition to in an SYBR Inexperienced-Based mostly Actual-Time RT-PCR.

A easy methodology for detection of a novel coronavirus (SARS-CoV-2) utilizing onestep RTPCR adopted by restriction fragment size polymorphism

A novel coronavirus related to acute respiratory illness (named SARS-CoV-2) is not too long ago recognized in Wuhan metropolis, China, unfold quickly worldwide. Early identification of this novel coronavirus by molecular instruments is essential for surveillance and management of the epidemic outbreak. We aimed to determine a easy methodology for the detection of SARS-CoV-2 in differentiating with SARS-CoV. Primers of our in-house reverse transcription polymerase chain response (RT-PCR) assays have been designed to focus on conserved areas of the RdRP gene and E gene, chosen restriction enzymes EcoRI, Tsp45I, and AluI to differentiate between SARS-CoV-2 and SARS-CoV.

Probe One-Step qRT-PCR SuperMix

20-abx09804220ulSystems
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Green One-Step qRT-PCR SuperMix

20-abx09803820ulSystems
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Probe One-Step qRT-PCR SuperMix (GC Rich)

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Green One-Step qRT-PCR SuperMix (GC Rich)

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Green Two-Step qRT-PCR SuperMix

abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)
EUR 777.6

Green miRNA Two-Step qRT-PCR SuperMix

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EUR 1062

Green Two-Step qRT-PCR SuperMix (GC Rich)

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PCR SuperMix

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  • EUR 326.40
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  • 1 ml
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One-Step RT-PCR SuperMix (+dye) (4 kb)

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EUR 727.2

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098028-200rxns20ulSystems 200 rxns × 20 ul Systems
EUR 794.4

One-Step RT-PCR SuperMix (+dye) (8 kb)

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EUR 1028.4

PCR SuperMix (+dye)

20-abx098003
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  • EUR 226.80
  • EUR 7408.80
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  • 15 ml
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  • 5 ml

PCR SuperMix (-dye)

20-abx098002
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  • 15 ml
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Plus PCR SuperMix

20-abx098888
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  • EUR 560.40
  • 1 ml
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Pro QPCR SuperMix Kit

K5053200 200 reactions
EUR 161

Pro QPCR SuperMix Kit

K5053400 400 reactions
EUR 291

T PCR SuperMix (-dye)

20-abx098005
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  • EUR 410.40
  • 1 ml
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T PCR SuperMix (+dye)

20-abx098006
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  • 1 ml
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EasyPfu PCR SuperMix (-dye)

20-abx098009
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  • 1 ml
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FastPfu PCR SuperMix (-dye)

20-abx098010
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  • EUR 594.00
  • 1 ml
  • 5 ml

Two-Step RT-PCR SuperMix (12 kb)

abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)
EUR 727.2

Two-Step RT-PCR SuperMix (15 kb)

abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)
EUR 910.8

PCR SuperMix for PAGE (+dye)

20-abx098004
  • EUR 493.20
  • EUR 226.80
  • EUR 309.60
  • 15 ml
  • 1 ml
  • 5 ml

Pro QPCR SuperMix Kit - ROX premixed

K5056200 200 reactions
EUR 161

Pro QPCR SuperMix Kit - ROX premixed

K5056400 400 reactions
EUR 291

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058200 200 reactions
EUR 188

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058400 400 reactions
EUR 316

One-Step gDNA Removal and cDNA Synthesis SuperMix

20-abx09885920ulSystems
  • EUR 1228.80
  • EUR 895.20
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High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)

20-abx098007
  • EUR 260.40
  • EUR 427.20
  • 1 ml
  • 5 ml

One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb)

20-abx09802220ulSystems
  • EUR 1128.00
  • EUR 828.00
  • 100 rxns × 20 ul Systems
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One Step MultiPlex qRT PCR Kit with Probe

9K-005-0012 100Rxn, 100prep
EUR 372.16

One-Step gDNA Removal and cDNA Synthesis SuperMix (8 kb)

20-abx09801520ulSystems
  • EUR 693.60
  • EUR 594.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

One-Step gDNA Removal and cDNA Synthesis SuperMix (12 kb)

20-abx09801720ulSystems
  • EUR 794.40
  • EUR 644.40
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Eva QPCR SuperMix Kit

K5052200 200 reactions
EUR 188

Eva QPCR SuperMix Kit

K5052400 400 reactions
EUR 316

HiScript II One Step qRT-PCR Probe Kit

Q222-01 100 rxn (50 μl/rxn)
EUR 396

HiScript II One Step qRT-PCR Probe Kit

Q223-EN01 250 rxns (20 μl/rxn)
EUR 125.6

First-Strand cDNA Synthesis SuperMix for PCR

abx098018-50rxns20ulSystems 50 rxns × 20 ul Systems
EUR 693.6

HiScript III One Step qRT-PCR Probe Kit

Q225-EN01 100 rxns
EUR 75.37

HiScript III One Step qRT-PCR Probe Kit

Q225-EN02 1,000 rxns
EUR 568.14

HiScript III One Step qRT-PCR Probe Kit

Q225-EN03 5,000 rxns
EUR 2608.8

Fast Eva QPCR SuperMix Kit

K5052002 200 rxn
EUR 188

Power Eva QPCR SuperMix Kit

K5057200 200 reactions
EUR 161

Power Eva QPCR SuperMix Kit

K5057400 400 reactions
EUR 316

All-in-One First-Strand cDNA Synthesis SuperMix for PCR

abx098023-50rxns20ulSystems 50 rxns × 20 ul Systems
EUR 878.4

HiScript II U+ One Step qRT-PCR Probe Kit

Q223-01 100 rxn (50 μl/rxn)
EUR 444

OneScriptcDNA Synthesis SuperMix

G451 25 x 20 ul reactions
EUR 106.8

OneScriptcDNA Synthesis SuperMix

G452 100 x 20 ul reactions
EUR 169.2

HiScript II One Step qRT-PCR SYBR Green Kit

Q221-01 100 rxn (50 μl/rxn)
EUR 396

Probe qPCR SuperMix

20-abx098040
  • EUR 1245.60
  • EUR 526.80
  • EUR 760.80
  • 15 ml
  • 1 ml
  • 5 ml

Evo? cDNA Supermix

M1168-100 each
EUR 457.2

Evo? cDNA Supermix

M1168-25 each
EUR 320.4

Novo? cDNA Supermix

M1169-100 each
EUR 529.2

Novo? cDNA Supermix

M1169-25 each
EUR 346.8

Green qPCR SuperMix

20-abx098031
  • EUR 1245.60
  • EUR 526.80
  • EUR 760.80
  • 15 ml
  • 1 ml
  • 5 ml

OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 116.4

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 202.8

High Fidelity (HiFi) PCR SuperMix (-dye) (lambda DNA, cDNA and plasmid DNA)

20-abx098008
  • EUR 260.40
  • EUR 427.20
  • 1 ml
  • 5 ml

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 678.00
  • EUR 577.20
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Green qPCR SuperMix UDG

20-abx098032
  • EUR 1362.00
  • EUR 543.60
  • EUR 794.40
  • 15 ml
  • 1 ml
  • 5 ml

Top Green qPCR SuperMix

20-abx098033
  • EUR 994.80
  • EUR 260.40
  • EUR 1362.00
  • EUR 493.20
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Tip Green qPCR SuperMix

20-abx098034
  • EUR 1161.60
  • EUR 276.00
  • EUR 1596.00
  • EUR 560.40
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

All-in-One cDNA Synthesis SuperMix

B24403 200 ractions
EUR 547.2
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix

B24408 1000 ractions
EUR 1743.6
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

Library Amplification SuperMix

20-abx098894
  • EUR 309.60
  • EUR 644.40
  • 1 ml
  • 5 ml

HiScript III One Step qRT-PCR Probe 5 × Master Mix

Q611-EN01 100 rxns (20 μl/rxn)
EUR 86.95

HiScript III One Step qRT-PCR Probe 5 × Master Mix

Q611-EN02 1,000 rxns (20 μl/rxn)
EUR 724.62

HiScript III One Step qRT-PCR Probe 5 × Master Mix

Q611-EN03 10,000 rxns (20 μl/rxn)
EUR 6086.8

cDNA Synthesis SuperMix for qPCR

20-abx09801920ulSystems
  • EUR 828.00
  • EUR 678.00
  • 100 rxns ×20 ul Systems
  • 50 rxns × 20 ul Systems

Top Green qPCR SuperMix (+Dye II)

20-abx098890
  • EUR 994.80
  • EUR 260.40
  • EUR 1362.00
  • EUR 493.20
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Tip Green qPCR SuperMix (+Dye II)

20-abx098891
  • EUR 1161.60
  • EUR 276.00
  • EUR 1596.00
  • EUR 560.40
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Library Quantification qPCR SuperMix

20-abx098896
  • EUR 276.00
  • EUR 493.20
  • 1 ml
  • 5 ml

HiScript II Q RT SuperMix for qPCR

R222-01 100 rxn (20 μl/rxn)
EUR 282

Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye

MB1004-100Reactions 100 Reactions
EUR 392.4

Fly First-Strand cDNA Synthesis SuperMix

20-abx09885820ulSystems
  • EUR 861.60
  • EUR 693.60
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

cDNA Synthesis SuperMix for qPCR (GC-rich)

abx098024-50rxns20ulSystems 50 rxns × 20 ul Systems
EUR 861.6

miRNA First-Strand cDNA Synthesis SuperMix

abx098020-20rxns20ulSystems 20 rxns × 20 ul Systems
EUR 878.4

HiScript III All-in-one RT SuperMix Perfect for qPCR

R333-01 100 rxns (21 μl/rxn)
EUR 174

HiScript II Q Select RT SuperMix for qPCR

R232-01 100 rxn (20 μl/rxn)
EUR 282

HiScript III RT SuperMix for qPCR (+g DNA wiper)

R323-01 100 rxn (20 μl/rxn)
EUR 351.6

One-Step TaqProbe qRT-PCR-ROX

G493-P 100 rxn (20 ul/rxn)
EUR 189.6

One-Step BrightGreen qRT-PCR-ROX

G471-R 100 rxn (20 ul/rxn)
EUR 189.6

One-Step TaqProbe qRT-PCR-iCycler

G493-PC 100 rxn (20 ul/rxn)
EUR 189.6

One-Step BrightGreen qRT-PCR-iCycler

G471-iC 100 rxn (20 ul/rxn)
EUR 189.6

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems
  • EUR 744.00
  • EUR 627.60
  • 100 rxns × 20 ul Systems
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First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems
  • EUR 1045.20
  • EUR 794.40
  • 100 rxns × 20 ul Systems
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HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper)

R233-01 100 rxn (20 μl/rxn)
EUR 292.8

One Step RT PCR Kit

9K-005-0011 100Rxn, 100prep
EUR 321

One-Step TaqProbe qRT-PCR-No Dye

G493-PS 100 rxn (20 ul/rxn)
EUR 189.6

One-Step TaqProbe qRT-PCR-Low ROX

G493-PL 100 rxn (20 ul/rxn)
EUR 189.6

One-Step BrightGreen qRT-PCR-No Dye

G471-S 100 rxn (20 ul/rxn)
EUR 189.6

One-Step BrightGreen qRT-PCR-Low ROX

G471-LR 100 rxn (20 ul/rxn)
EUR 189.6

ExCellenCT One-Step TaqProbe qRT-PCR-ROX

G918-P 25 Preps 100 x 20 ul reactions
EUR 212.4

Green-2-Go 1-Step qRT-PCR Mastermix-iCycler

QPCR011-iC 100Rxn
EUR 287.08

ExCellenCT One-Step BrightGreen qRT-PCR-ROX

G917-R 25 Preps 100 x 20 ul reactions
EUR 212.4

ExCellenCT One-Step TaqProbe qRT-PCR-iCycler

G918-PC 25 Preps 100 x 20 ul reactions
EUR 212.4

ExCellenCT One-Step BrightGreen qRT-PCR-iCycler

G917-iC 25 Preps 100 x 20 ul reactions
EUR 212.4

T-Pro Gel/PCR DNA Purification Mini Kit (100)

RB94-NES100 100preps/Kit
EUR 193.2

T-Pro Gel/PCR DNA Purification Mini Kit (250)

RB94-NES250 250preps/Kit
EUR 266.4

T-Pro Gel/PCR DNA Purification Maxi Kit (10)

RB94-NEL010 10preps/Kit
EUR 193.2

Green-2-Go 1-Step qRT-PCR Mastermix-Low Rox

QPCR012-LR 100Rxn
EUR 287.08

ExCellenCT One-Step TaqProbe qRT-PCR-No Dye

G918-PS 25 Preps 100 x 20 ul reactions
EUR 212.4

ExCellenCT One-Step TaqProbe qRT-PCR-Low ROX

G918-PL 25 Preps 100 x 20 ul reactions
EUR 212.4

ExCellenCT One-Step BrightGreen qRT-PCR-No Dye

G917-S 25 Preps 100 x 20 ul reactions
EUR 212.4

ExCellenCT One-Step BrightGreen qRT-PCR-Low ROX

G917-LR 25 Preps 100 x 20 ul reactions
EUR 212.4

Green-2-Go 1-Step qRT-PCR Mastermix-Rox (100X20ul Rxn)

QPCR010-R 100Rxn
EUR 287.08

One Step RT PCR Kit (cDNA Synthesis + PCR In One Reaction)

MB1003-100Reactions 100 Reactions
EUR 895.2

Green-2-Go 1-Step qRT-PCR Mastermix-No Dye (100X20ul Rxn)

QPCR007-NODYE 100Rxn
EUR 287.08

One Step AMV RT-PCR Kit

BS6649 100preps
EUR 477.6

AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE)

Q621-EN01 200 rxns (20 μl/rxn)
EUR 208.69

AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE)

Q621-EN02 1,000 rxns (20 μl/rxn)
EUR 811.58

AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE)

Q621-EN03 10,000 rxns (20 μl/rxn)
EUR 6956.4

One Step M-MULV RT-PCR Kit

BS665 100preps
EUR 477.6

HiScipt II One Step RT-PCR Kit

P611-01 50 rxn (50 μl/rxn)
EUR 234

Green-2-Go 1-Step TaqProbe qRT-PCR Mastermix-no Dye (100X20ul Rxn)

QPCR008-NODYE 100Rxn
EUR 341.88

igScript One Step RT-PCR Kit - 100 Reactions

4211 1/EA
EUR 284.4

igScript One Step RT-PCR Kit - 500 Reactions

4213 1/EA
EUR 908.4

T-Pro BCA Protein Assay kit

JB04-D001 500assay/KIT
EUR 244.8

HiScipt II One Step RT-PCR Kit (Dye Plus)

P612-01 50 rxn (50 μl/rxn)
EUR 234

T-Pro Bradford Protein Assay kit(1X)

JB04-D002 500ml/BT
EUR 193.2

T-Pro Plasmid Mini Kit (100)

RB94-YPD100 100preps/Kit
EUR 193.2

T-Pro Plasmid Mini Kit (250)

RB94-YPD250 250preps/Kit
EUR 266.4

T-Pro Plasmid Midi Kit (20)

RB94-YPI020 20preps/Kit
EUR 255.6

T-Pro Plasmid Maxi Kit (10)

RB94-YPM010 10preps/Kit
EUR 266.4

T-Pro Genomic DNA Mini Kit (100)

RB94-NGS100 100preps/kit
EUR 255.6

T-Pro Genomic DNA Midi Kit (20)

RB94-NGM020 20preps/kit
EUR 266.4

T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit)

JT96-K002M 250ml*2/Kit
EUR 244.8

T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit)

JT96-K002S 100ml*2/Kit
EUR 182.4

T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)

JT96-K004M 250ml*2/Kit
EUR 286.8

T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)

JT96-K004S 100ml*2/Kit
EUR 204

T-Pro Plant Genomic DNA Mini Kit (100)

RB94-PGS100 100preps/kit
EUR 266.4

T-Pro Plant Genomic DNA Midi Kit (20)

RB94-PGM020 20preps/kit
EUR 266.4

T-Pro Endotoxin Removal Plasmid Midi kit (25)

RB94-EPI020 20preps/Kit
EUR 297.6

T-Pro Endotoxin Removal Plasmid Maxi kit (10)

RB94-EPM010 10preps/Kit
EUR 318

ranavirus One-Step PCR kit

Oneq-V337-100D 100T
EUR 1039.2

ranavirus One-Step PCR kit

Oneq-V337-150D 150T
EUR 1177.2

ranavirus One-Step PCR kit

Oneq-V337-50D 50T
EUR 861.6

Sapovirus One-Step PCR kit

Oneq-H630-100R 100T
EUR 1932

Sapovirus One-Step PCR kit

Oneq-H630-150R 150T
EUR 2646

Sapovirus One-Step PCR kit

Oneq-H630-50R 50T
EUR 1410

Entamoeba One-Step PCR kit

Oneq-H683-100D 100T
EUR 1039.2

Entamoeba One-Step PCR kit

Oneq-H683-150D 150T
EUR 1177.2

Entamoeba One-Step PCR kit

Oneq-H683-50D 50T
EUR 861.6

Echovirus One-Step PCR kit

Oneq-H722-100D 100T
EUR 1039.2

Echovirus One-Step PCR kit

Oneq-H722-150D 150T
EUR 1177.2

Echovirus One-Step PCR kit

Oneq-H722-50D 50T
EUR 861.6

Mengovirus One-Step PCR kit

Oneq-H453-100R 100T
EUR 1932

Mengovirus One-Step PCR kit

Oneq-H453-150R 150T
EUR 2646

Mengovirus One-Step PCR kit

Oneq-H453-50R 50T
EUR 1410

Astrovirus One-Step PCR kit

Oneq-H491-100R 100T
EUR 1932

Astrovirus One-Step PCR kit

Oneq-H491-150R 150T
EUR 2646

Astrovirus One-Step PCR kit

Oneq-H491-50R 50T
EUR 1410

Adenovirus One-Step PCR kit

Oneq-H501-100D 100T
EUR 1039.2

Adenovirus One-Step PCR kit

Oneq-H501-150D 150T
EUR 1177.2

Adenovirus One-Step PCR kit

Oneq-H501-50D 50T
EUR 861.6

On this report, a 396-bp fragment of the RdRp gene and 345-bp fragment of the E gene have been amplified by one-step RT-PCR. Enzyme Tsp45I cuts the RdRP-amplified product of SARS-CoV-2 producing three fragments of 45, 154, and 197 bp, but it surely didn’t reduce the amplicon of SARS-CoV. In distinction, the amplified product of SARS-CoV was digested with EcoRI producing two fragments of 76 and 320 bp, whereas the amplicon of SARS-CoV-2 was undigested by Tsp45I assist to differentiate clearly SARS-CoV-2 from SARS-CoV on gel electrophoresis.

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