Development of a New Internally Controlled One-Step Real-Time RT-PCR for the Molecular Detection of Enterovirus A71 in Africa and Madagascar

Development of a New Internally Controlled One-Step Real-Time RT-PCR for the Molecular Detection of Enterovirus A71 in Africa and Madagascar

Enterovirus A71 (EV-A71) is a number one reason behind hand-foot-and-mouth illness (HFMD) and might be related to extreme neurological problems. EV-A71 strains might be labeled into seven genogroups, A-H, on the idea of the VP1 capsid protein gene sequence. Genogroup A contains the prototype pressure; genogroups B and C are accountable of main outbreaks worldwide, however little is understood in regards to the others, notably genogroups E and F, which have been not too long ago recognized in Africa and Madagascar, respectively. The circulation of EV-A71 within the African area is poorly recognized and possibly underestimated. A speedy and particular assay for detecting all genogroups of EV-A71 is required.

On this examine, we developed a real-time RT-PCR assay with a aggressive inner management (IC). The primers and TaqMan probe particularly goal the genomic area encoding the VP1 capsid protein. Various EV-A71 RNAs have been efficiently amplified from the genogroups A, B, C, D, E, and F, with related sensitivity and strong reproducibility. Neither cross response with different EVs nor main interference with the aggressive IC was detected. Experimentally spiked stool and plasma specimens offered constant and reproducible outcomes, and validated the usefulness of the IC for demonstrating the presence of PCR inhibitors in samples.

The evaluation in an African laboratories community of 1889 untyped enterovirus isolates detected 15 EV-A71 of various genogroups. This particular real-time RT-PCR assay supplies a strong and delicate methodology for the detection of EV-A71 in organic specimens and for the epidemiological monitoring of EV-A71 together with its not too long ago found genogroups.

Formalin-fixed paraffin-embedded (FFPE) tissues are an vital supply for investigation of dengue virus (DENV) an infection, notably when blood or recent frozen (FF) samples are unavailable. Histopathologic options and immunohistochemistry could have poor sensitivity and serotype willpower is just not at all times doable. Viral RNA genome detection assessments are sooner and regarded probably the most delicate approach for this sort of evaluation, nonetheless, the usage of molecular strategies utilized to FFPE tissues remains to be restricted.

The authors utilized a single-step multiplex reverse transcription-quantitative polymerase chain response (RT-qPCR) for the investigation of DENV an infection and typing to FFPE samples of 32 deadly circumstances acquired throughout the 2019 outbreak that occurred in São Paulo state, Brazil. The authors in contrast the outcomes with these obtained utilizing FF tissues. Of the 24 circumstances with each FF and FFPE samples, 22 (91.67%) of the FF and 19 (76.20%) of the FFPE specimens have been optimistic. Two circumstances (8.33%) examined unfavourable in each forms of samples.

onestepone-tube real-time RTPCR primarily based assay with an automatic evaluation for detection of SARS-CoV-2

Early analysis of SARS-CoV-2 contaminated sufferers is important to regulate the dynamics of the COVID-19 pandemic. We develop a speedy and correct one-step multiplex TaqMan probe-based real-time RT-PCR assay, together with a computational software to systematically analyse the info. Our assay may detect to a restrict of 15 copies of SARS-CoV-2 transcripts-based on experiments carried out by spiking whole human RNA with in vitro synthesized viral transcripts. The assay was evaluated by performing 184 validations for the SARS-CoV-2 Nucleocapsid gene and human RNase P as an inner management reference gene with dilutions starting from 1-100 ng for human RNA on a cohort of 26 medical samples. 5 of 26 sufferers have been confirmed to be contaminated with SARS-CoV-2, whereas 21 examined unfavourable, in keeping with the requirements.

The accuracy of the assay was discovered to be 100% delicate and 100% particular primarily based on the 26 medical samples that must be additional verified utilizing a lot of medical samples. In abstract, we current a speedy, simple to implement real-time PCR primarily based assay with automated evaluation utilizing a novel COVID qPCR Analyzer software with graphical person interface (GUI) to research the uncooked qRT-PCR information in an unbiased method at a value of underneath $Three per response and turnaround time of lower than 2h, to allow in-house SARS-CoV-2 testing throughout laboratories.

Development of a New Internally Controlled One-Step Real-Time RT-PCR for the Molecular Detection of Enterovirus A71 in Africa and Madagascar

The nucleotide area within the ORF1ab gene that has the best variations between the human coronavirus and the bat coronavirus was chosen. Primers have been designed after that sequence. All diagnostic primers are species-specific because the 3´ finish of the sequence differs from that of different species. A primer set additionally creates an artificial optimistic management. Amplified merchandise have been seen in a 2.5% agarose gel, in addition to in an SYBR Inexperienced-Based mostly Actual-Time RT-PCR.

A easy methodology for detection of a novel coronavirus (SARS-CoV-2) utilizing onestep RTPCR adopted by restriction fragment size polymorphism

A novel coronavirus related to acute respiratory illness (named SARS-CoV-2) is not too long ago recognized in Wuhan metropolis, China, unfold quickly worldwide. Early identification of this novel coronavirus by molecular instruments is essential for surveillance and management of the epidemic outbreak. We aimed to determine a easy methodology for the detection of SARS-CoV-2 in differentiating with SARS-CoV. Primers of our in-house reverse transcription polymerase chain response (RT-PCR) assays have been designed to focus on conserved areas of the RdRP gene and E gene, chosen restriction enzymes EcoRI, Tsp45I, and AluI to differentiate between SARS-CoV-2 and SARS-CoV.

Green One-Step qRT-PCR SuperMix

20-abx09803820ulSystems
  • EUR 594.00
  • EUR 895.20
  • 100 rxns × 20 ul Systems
  • 400 rxns × 20 ul Systems

Probe One-Step qRT-PCR SuperMix

20-abx09804220ulSystems
  • EUR 710.40
  • EUR 1228.80
  • 100 rxns × 20 ul Systems
  • 400 rxns × 20 ul Systems

Green Two-Step qRT-PCR SuperMix

abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)
EUR 777.6

Green One-Step qRT-PCR SuperMix (GC Rich)

20-abx09803920ulSystems
  • EUR 710.40
  • EUR 1228.80
  • 100 rxns × 20 ul Systems
  • 400 rxns × 20 ul Systems

Probe One-Step qRT-PCR SuperMix (GC Rich)

20-abx09804120ulSystems
  • EUR 594.00
  • EUR 895.20
  • 100 rxns × 20 ul Systems
  • 400 rxns × 20 ul Systems

Green miRNA Two-Step qRT-PCR SuperMix

abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems 20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems)
EUR 1062

Green Two-Step qRT-PCR SuperMix (GC Rich)

abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)
EUR 961.2

One-Step BrightGreen qRT-PCR-iCycler

G471-iC 100 rxn (20 ul/rxn)
EUR 189.6

One-Step BrightGreen qRT-PCR-ROX

G471-R 100 rxn (20 ul/rxn)
EUR 189.6

One-Step TaqProbe qRT-PCR-ROX

G493-P 100 rxn (20 ul/rxn)
EUR 189.6

One-Step TaqProbe qRT-PCR-iCycler

G493-PC 100 rxn (20 ul/rxn)
EUR 189.6

One Step MultiPlex qRT PCR Kit with Probe

9K-005-0012 100Rxn, 100prep
EUR 372.16

HiScript II One Step qRT-PCR Probe Kit

Q222-01 100 rxn (50 μl/rxn)
EUR 396

One-Step BrightGreen qRT-PCR-Low ROX

G471-LR 100 rxn (20 ul/rxn)
EUR 189.6

One-Step BrightGreen qRT-PCR-No Dye

G471-S 100 rxn (20 ul/rxn)
EUR 189.6

One-Step TaqProbe qRT-PCR-Low ROX

G493-PL 100 rxn (20 ul/rxn)
EUR 189.6

One-Step TaqProbe qRT-PCR-No Dye

G493-PS 100 rxn (20 ul/rxn)
EUR 189.6

ExCellenCT One-Step BrightGreen qRT-PCR-iCycler

G917-iC 25 Preps 100 x 20 ul reactions
EUR 212.4

ExCellenCT One-Step BrightGreen qRT-PCR-ROX

G917-R 25 Preps 100 x 20 ul reactions
EUR 212.4

ExCellenCT One-Step TaqProbe qRT-PCR-ROX

G918-P 25 Preps 100 x 20 ul reactions
EUR 212.4

ExCellenCT One-Step TaqProbe qRT-PCR-iCycler

G918-PC 25 Preps 100 x 20 ul reactions
EUR 212.4

HiScript II One Step qRT-PCR SYBR Green Kit

Q221-01 100 rxn (50 μl/rxn)
EUR 396

HiScript II U+ One Step qRT-PCR Probe Kit

Q223-01 100 rxn (50 μl/rxn)
EUR 444

ExCellenCT One-Step BrightGreen qRT-PCR-Low ROX

G917-LR 25 Preps 100 x 20 ul reactions
EUR 212.4

ExCellenCT One-Step BrightGreen qRT-PCR-No Dye

G917-S 25 Preps 100 x 20 ul reactions
EUR 212.4

ExCellenCT One-Step TaqProbe qRT-PCR-Low ROX

G918-PL 25 Preps 100 x 20 ul reactions
EUR 212.4

ExCellenCT One-Step TaqProbe qRT-PCR-No Dye

G918-PS 25 Preps 100 x 20 ul reactions
EUR 212.4

One-Step RT-PCR SuperMix (+dye) (4 kb)

abx098027-200rxns20ulSystems 200 rxns × 20 ul Systems
EUR 727.2

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098028-200rxns20ulSystems 200 rxns × 20 ul Systems
EUR 794.4

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098029-200rxns20ulSystems 200 rxns × 20 ul Systems
EUR 1028.4

Two-Step RT-PCR SuperMix (12 kb)

abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)
EUR 727.2

Two-Step RT-PCR SuperMix (15 kb)

abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)
EUR 910.8

PCR SuperMix

20-abx098887
  • EUR 326.40
  • EUR 693.60
  • 1 ml
  • 5 ml

Pro QPCR SuperMix Kit

K5053200 200 reactions
EUR 225.6
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Pro QPCR SuperMix Kit

K5053400 400 reactions
EUR 358.8
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

PCR SuperMix (-dye)

20-abx098002
  • EUR 493.20
  • EUR 226.80
  • EUR 309.60
  • 15 ml
  • 1 ml
  • 5 ml

PCR SuperMix (+dye)

20-abx098003
  • EUR 493.20
  • EUR 226.80
  • EUR 7408.80
  • EUR 309.60
  • 15 ml
  • 1 ml
  • 480 ml
  • 5 ml

Plus PCR SuperMix

20-abx098888
  • EUR 292.80
  • EUR 560.40
  • 1 ml
  • 5 ml

Green-2-Go 1-Step qRT-PCR Mastermix-iCycler

QPCR011-iC 100Rxn
EUR 287.08

Green-2-Go 1-Step qRT-PCR Mastermix-Low Rox

QPCR012-LR 100Rxn
EUR 287.08

T PCR SuperMix (-dye)

20-abx098005
  • EUR 243.60
  • EUR 410.40
  • 1 ml
  • 5 ml

T PCR SuperMix (+dye)

20-abx098006
  • EUR 243.60
  • EUR 410.40
  • 1 ml
  • 5 ml

EasyPfu PCR SuperMix (-dye)

20-abx098009
  • EUR 243.60
  • EUR 410.40
  • 1 ml
  • 5 ml

FastPfu PCR SuperMix (-dye)

20-abx098010
  • EUR 292.80
  • EUR 594.00
  • 1 ml
  • 5 ml

Pro QPCR SuperMix Kit - ROX premixed

K5056200 200 reactions
EUR 225.6
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Pro QPCR SuperMix Kit - ROX premixed

K5056400 400 reactions
EUR 358.8
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

One Step RT PCR Kit

9K-005-0011 100Rxn, 100prep
EUR 321

Pospiviroid One-Step PCR kit

Oneq-A546-100R 100T
EUR 1932

Pospiviroid One-Step PCR kit

Oneq-A546-150R 150T
EUR 2646

Pospiviroid One-Step PCR kit

Oneq-A546-50R 50T
EUR 1410

Lactobacillus One-Step PCR kit

Oneq-A566-100D 100T
EUR 1039.2

Lactobacillus One-Step PCR kit

Oneq-A566-150D 150T
EUR 1177.2

Lactobacillus One-Step PCR kit

Oneq-A566-50D 50T
EUR 861.6

Lactococcus One-Step PCR kit

Oneq-A567-100D 100T
EUR 1039.2

Lactococcus One-Step PCR kit

Oneq-A567-150D 150T
EUR 1177.2

Lactococcus One-Step PCR kit

Oneq-A567-50D 50T
EUR 861.6

Leuconostoc One-Step PCR kit

Oneq-A569-100D 100T
EUR 1039.2

Leuconostoc One-Step PCR kit

Oneq-A569-150D 150T
EUR 1177.2

Leuconostoc One-Step PCR kit

Oneq-A569-50D 50T
EUR 861.6

Pediococcus One-Step PCR kit

Oneq-A571-100D 100T
EUR 1039.2

Pediococcus One-Step PCR kit

Oneq-A571-150D 150T
EUR 1177.2

Pediococcus One-Step PCR kit

Oneq-A571-50D 50T
EUR 861.6

Halobacillus One-Step PCR kit

Oneq-A635-100D 100T
EUR 1039.2

Halobacillus One-Step PCR kit

Oneq-A635-150D 150T
EUR 1177.2

Halobacillus One-Step PCR kit

Oneq-A635-50D 50T
EUR 861.6

Enterovirus One-Step PCR kit

Oneq-H433-100R 100T
EUR 1932

Enterovirus One-Step PCR kit

Oneq-H433-150R 150T
EUR 2646

Enterovirus One-Step PCR kit

Oneq-H433-50R 50T
EUR 1410

Mengovirus One-Step PCR kit

Oneq-H453-100R 100T
EUR 1932

Mengovirus One-Step PCR kit

Oneq-H453-150R 150T
EUR 2646

Mengovirus One-Step PCR kit

Oneq-H453-50R 50T
EUR 1410

Astrovirus One-Step PCR kit

Oneq-H491-100R 100T
EUR 1932

Astrovirus One-Step PCR kit

Oneq-H491-150R 150T
EUR 2646

Astrovirus One-Step PCR kit

Oneq-H491-50R 50T
EUR 1410

Calicivirus One-Step PCR kit

Oneq-H493-100R 100T
EUR 1932

Calicivirus One-Step PCR kit

Oneq-H493-150R 150T
EUR 2646

Calicivirus One-Step PCR kit

Oneq-H493-50R 50T
EUR 1410

Adenovirus One-Step PCR kit

Oneq-H501-100D 100T
EUR 1039.2

Adenovirus One-Step PCR kit

Oneq-H501-150D 150T
EUR 1177.2

Adenovirus One-Step PCR kit

Oneq-H501-50D 50T
EUR 861.6

Polyomavirus One-Step PCR kit

Oneq-H515-100D 100T
EUR 1039.2

Polyomavirus One-Step PCR kit

Oneq-H515-150D 150T
EUR 1177.2

Polyomavirus One-Step PCR kit

Oneq-H515-50D 50T
EUR 861.6

Ruminococcus One-Step PCR kit

Oneq-H619-100D 100T
EUR 1039.2

Ruminococcus One-Step PCR kit

Oneq-H619-150D 150T
EUR 1177.2

Ruminococcus One-Step PCR kit

Oneq-H619-50D 50T
EUR 861.6

Bifidobacterium One-Step PCR kit

Oneq-H621-100D 100T
EUR 1039.2

Bifidobacterium One-Step PCR kit

Oneq-H621-150D 150T
EUR 1177.2

Bifidobacterium One-Step PCR kit

Oneq-H621-50D 50T
EUR 861.6

Sapovirus One-Step PCR kit

Oneq-H630-100R 100T
EUR 1932

Sapovirus One-Step PCR kit

Oneq-H630-150R 150T
EUR 2646

Sapovirus One-Step PCR kit

Oneq-H630-50R 50T
EUR 1410

Parechovirus One-Step PCR kit

Oneq-H642-100R 100T
EUR 1932

Parechovirus One-Step PCR kit

Oneq-H642-150R 150T
EUR 2646

Parechovirus One-Step PCR kit

Oneq-H642-50R 50T
EUR 1410

Metapneumovirus One-Step PCR kit

Oneq-H643-100R 100T
EUR 1932

Metapneumovirus One-Step PCR kit

Oneq-H643-150R 150T
EUR 2646

Metapneumovirus One-Step PCR kit

Oneq-H643-50R 50T
EUR 1410

Entamoeba One-Step PCR kit

Oneq-H683-100D 100T
EUR 1039.2

Entamoeba One-Step PCR kit

Oneq-H683-150D 150T
EUR 1177.2

Entamoeba One-Step PCR kit

Oneq-H683-50D 50T
EUR 861.6

Acanthamoeba One-Step PCR kit

Oneq-H684-100D 100T
EUR 1039.2

Acanthamoeba One-Step PCR kit

Oneq-H684-150D 150T
EUR 1177.2

Acanthamoeba One-Step PCR kit

Oneq-H684-50D 50T
EUR 861.6

Echovirus One-Step PCR kit

Oneq-H722-100D 100T
EUR 1039.2

Echovirus One-Step PCR kit

Oneq-H722-150D 150T
EUR 1177.2

Echovirus One-Step PCR kit

Oneq-H722-50D 50T
EUR 861.6

ranavirus One-Step PCR kit

Oneq-V337-100D 100T
EUR 1039.2

ranavirus One-Step PCR kit

Oneq-V337-150D 150T
EUR 1177.2

ranavirus One-Step PCR kit

Oneq-V337-50D 50T
EUR 861.6

Ruminococcus One-Step PCR kit

Oneq-V619-100D 100T
EUR 896.88
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Ruminococcus One-Step PCR kit

Oneq-V619-150D 150T
EUR 1006.5
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Ruminococcus One-Step PCR kit

Oneq-V619-50D 50T
EUR 754.2
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Bifidobacterium One-Step PCR kit

Oneq-V621-100D 100T
EUR 896.88
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

Bifidobacterium One-Step PCR kit

Oneq-V621-150D 150T
EUR 1006.5
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

Bifidobacterium One-Step PCR kit

Oneq-V621-50D 50T
EUR 754.2
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

One-Step RT-PCR Kit

G174 100 x 50 ul reactions
EUR 279.6

T-Pro Gel/PCR DNA Purification Maxi Kit (10)

RB94-NEL010 10preps/Kit
EUR 193.2

T-Pro Gel/PCR DNA Purification Mini Kit (100)

RB94-NES100 100preps/Kit
EUR 193.2

T-Pro Gel/PCR DNA Purification Mini Kit (250)

RB94-NES250 250preps/Kit
EUR 266.4

QPCR Kit QRT-PCR Mastermix RNA

MOL6356 EACH
EUR 257.64

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 319.2

One-Step gDNA Removal and cDNA Synthesis SuperMix

20-abx09885920ulSystems
  • EUR 1228.80
  • EUR 895.20
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye

MB1004-100Reactions 100 Reactions
EUR 392.4

Green-2-Go 1-Step qRT-PCR Mastermix-Rox (100X20ul Rxn)

QPCR010-R 100Rxn
EUR 287.08

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058200 200 reactions
EUR 253.2
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058400 400 reactions
EUR 384
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

PCR SuperMix for PAGE (+dye)

20-abx098004
  • EUR 493.20
  • EUR 226.80
  • EUR 309.60
  • 15 ml
  • 1 ml
  • 5 ml

MyTaq One-Step RT-PCR Kit

BIO-65048 25 Reactions Ask for price

MyTaq One-Step RT-PCR Kit

BIO-65049 100 Reactions Ask for price

One Step AMV RT-PCR Kit

BS6649 100preps
EUR 477.6

Wallemia sebi One-Step PCR kit

Oneq-A076-100D 100T
EUR 1039.2

Wallemia sebi One-Step PCR kit

Oneq-A076-150D 150T
EUR 1177.2

Wallemia sebi One-Step PCR kit

Oneq-A076-50D 50T
EUR 861.6

Tenacibaculum maritimum One-Step PCR kit

Oneq-A133-100D 100T
EUR 1039.2

Tenacibaculum maritimum One-Step PCR kit

Oneq-A133-150D 150T
EUR 1177.2

Tenacibaculum maritimum One-Step PCR kit

Oneq-A133-50D 50T
EUR 861.6

Geotrichum candidum One-Step PCR kit

Oneq-A341-100D 100T
EUR 1039.2

Geotrichum candidum One-Step PCR kit

Oneq-A341-150D 150T
EUR 1177.2

Geotrichum candidum One-Step PCR kit

Oneq-A341-50D 50T
EUR 861.6

Beggiatoa alba One-Step PCR kit

Oneq-A350-100D 100T
EUR 1039.2

Beggiatoa alba One-Step PCR kit

Oneq-A350-150D 150T
EUR 1177.2

Beggiatoa alba One-Step PCR kit

Oneq-A350-50D 50T
EUR 861.6

Flexibacter sp. One-Step PCR kit

Oneq-A485-100D 100T
EUR 1039.2

Flexibacter sp. One-Step PCR kit

Oneq-A485-150D 150T
EUR 1177.2

Flexibacter sp. One-Step PCR kit

Oneq-A485-50D 50T
EUR 861.6

Thiothrix sp. One-Step PCR kit

Oneq-A526-100D 100T
EUR 1039.2

Thiothrix sp. One-Step PCR kit

Oneq-A526-150D 150T
EUR 1177.2

Thiothrix sp. One-Step PCR kit

Oneq-A526-50D 50T
EUR 861.6

Thysanophora penicilioides One-Step PCR kit

Oneq-A527-100D 100T
EUR 1039.2

Thysanophora penicilioides One-Step PCR kit

Oneq-A527-150D 150T
EUR 1177.2

Thysanophora penicilioides One-Step PCR kit

Oneq-A527-50D 50T
EUR 861.6

Streptococcus dysgalactiae One-Step PCR kit

Oneq-A529-100D 100T
EUR 1039.2

Streptococcus dysgalactiae One-Step PCR kit

Oneq-A529-150D 150T
EUR 1177.2

Streptococcus dysgalactiae One-Step PCR kit

Oneq-A529-50D 50T
EUR 861.6

On this report, a 396-bp fragment of the RdRp gene and 345-bp fragment of the E gene have been amplified by one-step RT-PCR. Enzyme Tsp45I cuts the RdRP-amplified product of SARS-CoV-2 producing three fragments of 45, 154, and 197 bp, but it surely didn’t reduce the amplicon of SARS-CoV. In distinction, the amplified product of SARS-CoV was digested with EcoRI producing two fragments of 76 and 320 bp, whereas the amplicon of SARS-CoV-2 was undigested by Tsp45I assist to differentiate clearly SARS-CoV-2 from SARS-CoV on gel electrophoresis.

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