Since 2014, porcine epidemic diarrhea virus (PEDV) has reemerged in Europe. RT-PCR strategies have been described for the detection of PEDV, however none have been validated in keeping with a norm. On this research we described the event and validation of a SYBR™ Inexperienced one-step RT-qPCR in keeping with the French norm NF U47-600, for the detection and quantification of PEDV viral RNA. The strategy was validated from pattern preparation (feces or jejunum) by way of to nucleic acid extraction and RT-qPCR detection. Specificity and sensitivity, restrict of detection (LoD), restrict of quantification (LQ), linearity, intra and inter assay variability had been evaluated utilizing transcribed RNA and fecal and jejunum matrices spiked with virus.
The analytical and diagnostic specificities and sensitivities of this RT-qPCR had been 100% on this research. A LoD of 50 genome copies/5 μl of extract from fecal matrices spiked with virus or RNA transcript and 100 genome copies/5 μl of extract from jejunum matrices spiked with virus had been obtained. The Decrease LQ (LLQ) was 100 genome copies/5 μl and the Higher LQ (ULQ) 108 copies/5 μl. This technique is the primary, validated in accordance a norm for PEDV and should function a worldwide reference technique to harmonize detection and quantification of PEDV viral RNA in each subject and experimental settings.
As well as, AluI minimize the amplicon of the E gene of SARS-CoV-2 producing two fragments of 248 and 97 bp with out chopping to SARS-CoV. The accuracy of the assay was confirmed by sequencing and phylogenetic evaluation. When evaluated on scientific samples confirmed a excessive sensitivity of 95%, specificity of our assay was 100% and scientific efficiency for detection of SARS-CoV-2 compared with different reference assays. In conclusion, within the current research, we efficiently developed a easy technique for molecular detection of SARS-CoV-2 in differentiating with SARS-CoV.
Detection of avian influenza virus and newcastle illness virus by duplex one step RT PCR.
Newcastle illness Virus (NDV), a member of the Paramyxoviridae household, and Influenza virus, from the Orthomyxoviridae household, are two most important avian pathogens that trigger critical financial issues in poultry farming. NDV strains are labeled into three main pathotypes: velogenic, mesogenic, and lentogenic. Avian influenza viruses (AIV) are additionally divided into: low pathogenic (LPAI) and extremely pathogenic (HPAI) strains. Each viruses are enveloped, single stranded, negative-sense RNA viruses which give comparable signs starting from sub-clinical infections to extreme illness, together with loss in egg manufacturing, acute respiratory syndrome, and excessive mortality, relying on their stage of pathogenicity.
This similarity hinders prognosis when based mostly solely on scientific and publish mortem examination. A lot of the at present obtainable molecular detection strategies are additionally pathogenspecific, in order that a couple of RT-PCR is then required to verify or exclude the presence of each pathogens. To beat this drawback, we now have utilized a One Step Duplex RT-PCR technique to tell apart between these two pathogens. The primary goal of the venture was to develop a common, quick, and cheap technique which could possibly be utilized in any veterinary laboratory.
Reducing H+ RT concentrations 10 to 104-fold from producer suggestions improved cDNA predictions (common 0.Eight cycles) and elevated RT-PCR assay effectivity. RT exercise assays and fashions can be utilized to assist assay design and enhance the pace of RT-PCRs. RT kind and focus should be chosen to advertise speedy cDNA synthesis however reduce nonspecific amplification. We display 2-minute one-step RT-PCR of a Zika virus goal utilizing decreased RT concentrations and excessive PCR.
Thermo-Responsive Polymer Capsules in Actual-Time One–Step RT–PCR for Extremely Multiplex RNA Evaluation.
Speedy and easy detection of RNA targets is in excessive demand because of the rising menace of pandemic viruses. One-step real-time, reverse transcription-polymerase chain response (One-step RT-qPCR) utilizing a managed launch system of thermo-responsive supplies is developed on this paper to allow high-fidelity RNA evaluation as suppressing by-products. The nanocapsules, consisting of higher important resolution temperature (UCST) materials and PCR primers, carry or launch the primers relying upon the temperature.
The united states nanocapsules are launched into hydrogel microparticles included with RT primers after which the goal RNA is selectively amplified within the microparticle by way of one-step RT-qPCR. Extreme aspect merchandise are sharply subdued by separating the PCR primers from the RT course of via the microparticles with nanocapsules. As a result of the one-step assay is now carried out in a single microparticle, a number of goal RNAs will be analyzed in a easy RT-qPCR of a number of particles. Dependable 18-plex one-step RT-qPCR is efficiently carried out inside 30 min utilizing single-color fluorescent optics.
This work additionally explains the facile fabrication processes used for the thermo-responsive nanocapsules and hydrogel microparticles by the mixing polymerization technique. Extensible multiplex evaluation of influenza virus demonstrates the versatile makes use of of this one-step RT-qPCR platform.
Growth and Analysis of a One–Step Quantitative RT–PCR Assay for Detection of Lassa Virus.
Lassa fever is a extreme viral hemorrhagic sickness brought on by Lassa virus. Based mostly on estimates, the variety of LASV infections ranges from 300,000 to 500,000 circumstances in endemic areas with a fatality price of 1%. Growth of quick and delicate instruments for the management and prevention of Lassa virus an infection in addition to for scientific diagnostics of Lassa fever are essential. Right here we reported improvement and analysis of a one-step quantitative RT-qPCR assay for the Lassa virus detection – LASV-Fl. This assay is appropriate for the detection of lineages I-IV of Lassa virus.
The restrict of detection of the assay ranged from 103 copies/ml to 105 copies/ml and has 96.4% diagnostic sensitivity, whereas analytical and diagnostic specificities each had been 100%. Serum, complete blood and tissue are appropriate to be used with the assay. The assay accommodates all the mandatory elements to carry out the evaluation, together with an armored optimistic management (ARC+) and an armored inside management (IC).
AzuraQuant cDNA Synthesis Kit - 100 Reactions |
|||
| AZ-1996 | Real Time Primers | 100 Reactions | EUR 446.4 |
Axygen Polyester ultra clear sealing film for real time PCR |
|||
| AXY2152 | Scientific Laboratory Supplies | PK100 | EUR 382.8 |
Eztime Real - time PCRPremix 2x,Taqman |
|||
| FYT105-100P | Yeastern Biotech | 100 Preps 1.25 ml | Ask for price |
AzuraQuant cDNA Synthesis Kit - 25 Reactions |
|||
| AZ-1995 | Real Time Primers | 25 Reactions | EUR 183.6 |
Eco 48 Real Time PCR system including software and loading dock |
|||
| MOL6348 | Scientific Laboratory Supplies | EACH | EUR 18466.8 |
Eztime Real - time PCRPremix 2x,SYBR Green |
|||
| FYT103-100P | Yeastern Biotech | 100 Preps 1.25 ml | Ask for price |
Eztime Real - time PCRPremix 2x,SYBR Green |
|||
| FYT103-400P | Yeastern Biotech | 400 Preps 1.25 ml x 4 | Ask for price |
Eztime Real - time PCRPremix 2x,Taqman, ROX |
|||
| FYT105-400P | Yeastern Biotech | 400 Preps 1.25 ml x 4 | Ask for price |
Eztime Real - time PCRPremix 2x,Taqman, ROX |
|||
| FYT106-100P | Yeastern Biotech | 100 Preps 1.25 ml | Ask for price |
Eztime Real - time PCRPremix 2x,SYBRGreen , ROX |
|||
| FYT104-100P | Yeastern Biotech | 100 Preps 1.25 ml | Ask for price |
Eztime Real - time PCRPremix 2x,SYBRGreen , ROX |
|||
| FYT104-400P | Yeastern Biotech | 400 Preps 1.25 ml x 4 | Ask for price |
hsa-mir-521 Real-time RT-PCR Detection Kit |
|||
| 20-abx097600 | Abbexa |
|
|
Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye |
|||
| MB1004-100Reactions | BosterBio | 100 Reactions | EUR 392.4 |
Novel Coronavirus COVID-19 (2019-nCoV) Real Time Multiplex RT-PCR Kit (Detection for 3 Genes ) |
|||
| RR-0479-02 | Liferiver | 25 tests/kit | EUR 469.56 |
|
Description: Novel Coronavirus (2019-nCoV) Real Time Multiplex RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. It detects N gene, E gene and RdRp gene of 2019-nCoV. RR-0479-02 has been also approverd by CFDA for emergency use and is WHO standard. |
|||
TurboQ Real-time PCR Machine with Laptop |
|||
| MOL2700 | Scientific Laboratory Supplies | EACH | EUR 33836.4 |
dsGreen for Real-Time PCR, 100×, 1 mL |
|||
| 41010 | Lumiprobe | 1 mL | EUR 298.8 |
dsGreen for Real-Time PCR, 100×, 10 mL |
|||
| 81010 | Lumiprobe | 10 mL | EUR 1042.8 |
dsGreen for Real-Time PCR, 100×, 5 mL |
|||
| 71010 | Lumiprobe | 5 mL | EUR 750 |
dsGreen for Real-Time PCR, 100×, 2 mL |
|||
| 61010 | Lumiprobe | 2 mL | EUR 435.6 |
Azura PureView 1 Kb DNA Ladder - 100 Lanes |
|||
| AZ-1101 | Real Time Primers | 100 Lanes | EUR 140.4 |
Azura PureView 1 Kb DNA Ladder - 500 Lanes |
|||
| AZ-1105 | Real Time Primers | 500 Lanes | EUR 277.2 |
Azura PureView 1 Kb DNA Ladder - 1000 Lanes |
|||
| AZ-1105-2 | Real Time Primers | 1000 Lanes | EUR 326.4 |
Azura PureView 250bp DNA Ladder - 100 Lanes |
|||
| AZ-1121 | Real Time Primers | 100 Lanes | EUR 140.4 |
Azura PureView 250bp DNA Ladder - 500 Lanes |
|||
| AZ-1125 | Real Time Primers | 500 Lanes | EUR 277.2 |
Azura PureView 250bp DNA Ladder - 1000 Lanes |
|||
| AZ-1125-2 | Real Time Primers | 1000 Lanes | EUR 326.4 |
Azura PureView 100bp DNA Ladder - 100 Lanes |
|||
| AZ-1131 | Real Time Primers | 100 Lanes | EUR 140.4 |
Azura PureView 100bp DNA Ladder - 500 Lanes |
|||
| AZ-1135 | Real Time Primers | 500 Lanes | EUR 277.2 |
Azura PureView 100bp DNA Ladder - 1000 Lanes |
|||
| AZ-1135-2 | Real Time Primers | 1000 Lanes | EUR 326.4 |
Azura PureView 50bp DNA Ladder - 100 Lanes |
|||
| AZ-1151 | Real Time Primers | 100 Lanes | EUR 140.4 |
Azura PureView 50bp DNA Ladder - 500 Lanes |
|||
| AZ-1155 | Real Time Primers | 500 Lanes | EUR 277.2 |
Azura PureView 50bp DNA Ladder - 1000 Lanes |
|||
| AZ-1155-2 | Real Time Primers | 1000 Lanes | EUR 326.4 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 200 Rxn |
|||
| AZ-2301 | Real Time Primers | 200 Rxn | EUR 214.8 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 500 Rxn |
|||
| AZ-2305 | Real Time Primers | 500 Rxn | EUR 384 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 2000 Rxn |
|||
| AZ-2320 | Real Time Primers | 2000 Rxn | EUR 1195.2 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 5000 Rxn |
|||
| AZ-2325 | Real Time Primers | 5000 Rxn | EUR 2817.6 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 200 Rxn |
|||
| AZ-2401 | Real Time Primers | 200 Rxn | EUR 214.8 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 500 Rxn |
|||
| AZ-2405 | Real Time Primers | 500 Rxn | EUR 384 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 2000 Rxn |
|||
| AZ-2420 | Real Time Primers | 2000 Rxn | EUR 1195.2 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 5000 Rxn |
|||
| AZ-2421 | Real Time Primers | 5000 Rxn | EUR 2817.6 |
Mouse Gdnf Signaling Primer Library |
|||
| MGDNF-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Glucose Metabolism Primer Library |
|||
| MGLM-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Hippo Signaling Primer Library |
|||
| MHPO-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Hypoxia Signaling Primer Library |
|||
| MHYP-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Notch Signaling Primer Library |
|||
| MNOT-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Neurotransmitter Signaling Primer Library |
|||
| MNTS-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Integrin Signaling Primer Library |
|||
| MINT-I | Real Time Primers | 1 set | EUR 891.6 |
Mouse Oxidative Stress Primer Library |
|||
| MOSL-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Oncogene Primer Library |
|||
| MONC-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Apoptosis Primer Library |
|||
| MPA-I | Real Time Primers | 1 set | EUR 540 |
Mouse Ras Signaling Primer Library |
|||
| MRAS-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse T-Cell Receptor Signaling Primer Library |
|||
| MTCR-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse TGF Beta Signaling Primer Library |
|||
| MTGFB-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Tumor Invasion/Metastasis Primer Library |
|||
| MTIM-I | Real Time Primers | 1 set | EUR 540 |
Mouse Tumor Immunity Primer Library |
|||
| MTIMU-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse p53 Signaling Primer Library |
|||
| MTPS-I | Real Time Primers | 1 set | EUR 540 |
Mouse WNT Signaling Primer Library |
|||
| MWNT-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Toll-like Receptor Signaling Primer Library |
|||
| newitem | Real Time Primers | 1 set | EUR 657.6 |
Mouse PI3K-AKT Signaling Primer Library |
|||
| MAKT-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Angiogenesis Primer Library |
|||
| MAPL-I | Real Time Primers | 1 set | EUR 540 |
Mouse Adherin - Tight Junction Primer Library |
|||
| MATJ-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Autophagy Primer Library |
|||
| MATPL-I | Real Time Primers | 1 set | EUR 540 |
Mouse Bdnf Signaling Primer Library |
|||
| MBDNF-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse BMP Signaling Primer Library |
|||
| MBMP-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Cytokine Primer Library I |
|||
| MCA-I | Real Time Primers | 1 set | EUR 540 |
Mouse Cytokine Primer Library II |
|||
| MCA-II | Real Time Primers | 1 set | EUR 540 |
Mouse Cell Cycle Primer Library |
|||
| MCC-I | Real Time Primers | 1 set | EUR 540 |
Mouse Cytokine and Chemokine Receptor Primer Library |
|||
| MCCR-I | Real Time Primers | 1 set | EUR 774 |
Mouse CREB Signaling Primer Library |
|||
| MCREB-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse DNA Damage Signaling Primer Library |
|||
| MDDS-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse DNA Repair Primer Library I |
|||
| MDRL-I | Real Time Primers | 1 set | EUR 774 |
Mouse DNA Repair Primer Library II |
|||
| MDRL-II | Real Time Primers | 1 set | EUR 774 |
Mouse Adipogenesis Primer Library |
|||
| MADG-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse EGFR Signaling Primer Library |
|||
| MEGFR-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Epithelial-Mesenchymal Transition Primer Library |
|||
| MEMT-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Estrogen Receptor Signaling Library |
|||
| MESR-I | Real Time Primers | 1 set | EUR 774 |
Mouse Fatty Liver Signaling Primer Library |
|||
| MFTL-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Jak/Stat Signaling Primer Library |
|||
| MJAK-I | Real Time Primers | 1 set | EUR 774 |
Mouse MAP Kinase Signaling Primer Library |
|||
| MMAP-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Myeloid Derived Suppressor Cell Primer Library |
|||
| MMDSC-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse NFKappaB Primer Library |
|||
| MNFKB-I | Real Time Primers | 1 set | EUR 540 |
Human DNA Repair Primer Library I |
|||
| HDRL-I | Real Time Primers | 1 set | EUR 657.6 |
Human DNA Repair Primer Library II |
|||
| HDRL-II | Real Time Primers | 1 set | EUR 657.6 |
Human Drug Transporter I Primer Library |
|||
| HDTP-I | Real Time Primers | 1 set | EUR 657.6 |
Human Drug Detoxication I |
|||
| HDTX-I | Real Time Primers | 1 set | EUR 657.6 |
Human Drug Detoxication II |
|||
| HDTX-II | Real Time Primers | 1 set | EUR 774 |
Human EGFR Signaling Primer Library |
|||
| HEGFR-I | Real Time Primers | 1 set | EUR 657.6 |
Human Epithelial Mesenchymal Transition Primer Library |
|||
| HEMT-I | Real Time Primers | 1 set | EUR 657.6 |
Human Endothelial Cell Primer Library |
|||
| HEND-I | Real Time Primers | 1 set | EUR 657.6 |
Human ERBB Signaling Primer Library |
|||
| HERBB-I | Real Time Primers | 1 set | EUR 657.6 |
Human Estrogen Signaling Primer Library |
|||
| HESR-I | Real Time Primers | 1 set | EUR 774 |
Human Focal Adhesion Signaling Primer Library |
|||
| HFCA-I | Real Time Primers | 1 set | EUR 657.6 |
Human FGF Receptor Signaling Primer Library |
|||
| HFGF-I | Real Time Primers | 1 set | EUR 774 |
Human Fatty Acid and Lipid Metabolism Primer Library |
|||
| HFLM-I | Real Time Primers | 1 set | EUR 657.6 |
Human Gastric Cancer Primer Library |
|||
| HGCPL-I | Real Time Primers | 1 set | EUR 657.6 |
Human GDNF Signaling Primer Library |
|||
| HGDNF-I | Real Time Primers | 1 set | EUR 657.6 |
Human Glucose Metabolism Primer Library |
|||
| HGLM-I | Real Time Primers | 1 set | EUR 540 |
Human G-Protein Coupled Receptor Signaling Primer Library |
|||
| HGPCR-I | Real Time Primers | 1 set | EUR 657.6 |
Human Hedgehog Signaling Primer Library |
|||
| HHOG-I | Real Time Primers | 1 set | EUR 774 |
Human Homeobox Gene Primer Library |
|||
| HHOX-I | Real Time Primers | 1 set | EUR 774 |
Human Hippo Signaling Primer Pathway |
|||
| HHPO-I | Real Time Primers | 1 set | EUR 657.6 |
Human Heat Shock Protein Primer Library |
|||
| HHSP-I | Real Time Primers | 1 set | EUR 891.6 |
Human Hypoxia Signaling Primer Library |
|||
| HHYP-I | Real Time Primers | 1 set | EUR 657.6 |
Human Interferon Type I Signaling Primer Library |
|||
| HIFN-I | Real Time Primers | 1 set | EUR 657.6 |
Human Interferon Type II Signaling Primer Library |
|||
| HIFN-II | Real Time Primers | 1 set | EUR 657.6 |
Human IL17 Signaling Primer Library |
|||
| HIL17-I | Real Time Primers | 1 set | EUR 657.6 |
Human Insulin Signaling Primer Library |
|||
| HINS-I | Real Time Primers | 1 set | EUR 657.6 |
Human Integrin Signaling Primer Library |
|||
| HINT-I | Real Time Primers | 1 set | EUR 657.6 |
Human Actin Cytoskeleton Signaling Primer Library |
|||
| HACS-I | Real Time Primers | 1 set | EUR 657.6 |
Human Adipogenesis Primer Library |
|||
| HADG-I | Real Time Primers | 1 set | EUR 657.6 |
Human Adaptive & Innate Immune Response Primer Library |
|||
| HAIIR-I | Real Time Primers | 1 set | EUR 657.6 |
Human PI3K-AKT Signaling Primer Library |
|||
| HAKT-I | Real Time Primers | 1 set | EUR 540 |
Human Amino Acid Metabolism Primer Library |
|||
| HAMM-I | Real Time Primers | 1 set | EUR 774 |
Human Androgen Signaling Primer Library |
|||
| HAND-I | Real Time Primers | 1 set | EUR 657.6 |
Human Angiogenesis Primer Library |
|||
| HAPL-I | Real Time Primers | 1 set | EUR 540 |
Human Antigen Processing and Presentation Primer Library |
|||
| HAPP-I | Real Time Primers | 1 set | EUR 657.6 |
Human Atherosclerosis Primer Library |
|||
| HATH-I | Real Time Primers | 1 set | EUR 657.6 |
Human Adherin - Tight Junction Primer Library |
|||
| HATJ-I | Real Time Primers | 1 set | EUR 657.6 |
Human Autophagy Primer Library |
|||
| HATPL-I | Real Time Primers | 1 set | EUR 540 |
Human Alzheimer's Disease Primer Library |
|||
| HAZD-I | Real Time Primers | 1 set | EUR 657.6 |
Human B-Cell Receptor Signaling Primer Library |
|||
| HBCR-I | Real Time Primers | 1 set | EUR 657.6 |
Human BDNF Signaling Primer Library |
|||
| HBDNF-I | Real Time Primers | 1 set | EUR 657.6 |
Human Bladder Cancer Primer Library |
|||
| HBLCP-I | Real Time Primers | 1 set | EUR 657.6 |
Human BMP Signaling Primer Library |
|||
| HBMP-I | Real Time Primers | 1 set | EUR 657.6 |
Human Brain Cancer Primer Library |
|||
| HBRC-I | Real Time Primers | 1 set | EUR 657.6 |
Human Breast Cancer Signaling Primer Library |
|||
| HBRCA-I | Real Time Primers | 1 set | EUR 657.6 |
Human Cytokine Primer Library I |
|||
| HCA-I | Real Time Primers | 1 set | EUR 540 |
Human Cytokine Primer Library II |
|||
| HCA-II | Real Time Primers | 1 set | EUR 540 |
Human Cell Adhesion Primer Library |
|||
| HCAD-I | Real Time Primers | 1 set | EUR 774 |
Human cAMP Signaling Primer Library |
|||
| HCAMP-I | Real Time Primers | 1 set | EUR 657.6 |
Human Cancer Pathway Primer Library |
|||
| HCAN-I | Real Time Primers | 1 set | EUR 657.6 |
Human Calcium Regulation Pathway |
|||
| HCAR-I | Real Time Primers | 1 set | EUR 657.6 |
Human Cell Cycle Primer Library |
|||
| HCC-I | Real Time Primers | 1 set | EUR 540 |
Human Colon Cancer Primer Library I |
|||
| HCCP-I | Real Time Primers | 1 set | EUR 657.6 |
Human Colon Cancer Primer Library II |
|||
| HCCP-II | Real Time Primers | 1 set | EUR 657.6 |
Human Cytokine and Chemokine Receptor Primer Library |
|||
| HCCR-I | Real Time Primers | 1 set | EUR 774 |
Human Cancer Driver Gene I Primer Library |
|||
| HCDG-I | Real Time Primers | 1 set | EUR 774 |
Human Cancer Driver Gene II Primer Library |
|||
| HCDG-II | Real Time Primers | 1 set | EUR 774 |
Human Chromatin Regulation Primer Library |
|||
| HCHR-I | Real Time Primers | 1 set | EUR 774 |
Human Oncogene circRNA Library |
|||
| HCirc-Onc-I | Real Time Primers | 1 set | EUR 1125.6 |
Human CREB Signaling Primer Library |
|||
| HCREB-I | Real Time Primers | 1 set | EUR 657.6 |
Human JAK/STAT Signaling Primer Library |
|||
| HJAK-I | Real Time Primers | 1 set | EUR 657.6 |
Human Kinase Library I |
|||
| HKIN-I | Real Time Primers | 1 set | EUR 540 |
Human Kinase Library II |
|||
| HKIN-II | Real Time Primers | 1 set | EUR 540 |
Human Kinase Library III |
|||
| HKIN-III | Real Time Primers | 1 set | EUR 540 |
Human Kinase Library IV |
|||
| HKIN-IV | Real Time Primers | 1 set | EUR 540 |
Human Kinase Library (I, II, III and IV) |
|||
| HKIN-X | Real Time Primers | 1 set | EUR 1710 |
Human Liver Cancer Primer Library |
|||
| HLCPL-I | Real Time Primers | 1 set | EUR 657.6 |
Human Ligand Gated Ion Channels Primer Library |
|||
| HLGI-I | Real Time Primers | 1 set | EUR 891.6 |
Human MAP Kinase Signaling Library |
|||
| HMAP-I | Real Time Primers | 1 set | EUR 657.6 |
Human Myeloid Derived Suppressor Cell Primer Library |
|||
| HMDSC-I | Real Time Primers | 1 set | EUR 657.6 |
Human Melanoma Primer Library |
|||
| HMEL-I | Real Time Primers | 1 set | EUR 657.6 |
Human Necroptosis Primer Library |
|||
| HNCR-I | Real Time Primers | 1 set | EUR 657.6 |
Human NFKappaB Primer Library |
|||
| HNFKB-I | Real Time Primers | 1 set | EUR 540 |
Human NK Cell Mediated Toxicity Primer Library |
|||
| HNKT-I | Real Time Primers | 1 set | EUR 657.6 |
Human Nitric Oxide Signaling Primer Library |
|||
| HNOS-I | Real Time Primers | 1 set | EUR 657.6 |
Human Notch Signaling Pathway |
|||
| HNOT-I | Real Time Primers | 1 set | EUR 657.6 |
Human Neurotransmitter Signaling Primer Library |
|||
| HNTS-I | Real Time Primers | 1 set | EUR 657.6 |
Human Oncogene Primer Library |
|||
| HONC-I | Real Time Primers | 1 set | EUR 657.6 |
Human Oxidative Stress Primer Library |
|||
| HOSL-I | Real Time Primers | 1 set | EUR 540 |
Human Osteogenesis Primer Library |
|||
| HOST-I | Real Time Primers | 1 set | EUR 657.6 |
Human Ovarian Cancer Primer Library |
|||
| HOVCL-I | Real Time Primers | 1 set | EUR 657.6 |
Human Apoptosis Primer Library |
|||
| HPA-I | Real Time Primers | 1 set | EUR 540 |
Human Pancreatic Cancer Primer Library |
|||
| HPANC-I | Real Time Primers | 1 set | EUR 657.6 |
Human PDGF Signaling Primer Library |
|||
| HPDGF-I | Real Time Primers | 1 set | EUR 657.6 |
Human Parkinson's Disease Primer Library |
|||
| HPKD-I | Real Time Primers | 1 set | EUR 657.6 |
Human Prostate Cancer Primer Library |
|||
| HPRC-I | Real Time Primers | 1 set | EUR 657.6 |
Human Ras Signaling Primer Library |
|||
| HRAS-I | Real Time Primers | 1 set | EUR 657.6 |
Human Retinoblastoma Gene Primer Library |
|||
| HRBG-I | Real Time Primers | 1 set | EUR 657.6 |
Human Rho GTPase-Signaling Primer Library |
|||
| HRGTP-I | Real Time Primers | 1 set | EUR 774 |
Human Cytoskeleton Primer Library |
|||
| HSCK-I | Real Time Primers | 1 set | EUR 774 |
Human Stem Cell Primer Library |
|||
| HSCL-I | Real Time Primers | 1 set | EUR 540 |
Human SMAD Signaling Primer Library |
|||
| HSMAD-I | Real Time Primers | 1 set | EUR 657.6 |
Human Statin Signaling Library |
|||
| HSTAT-I | Real Time Primers | 1 set | EUR 657.6 |
Human Signal Transduction Primer Library |
|||
| HSTL-I | Real Time Primers | 1 set | EUR 540 |
Human T-Cell Anergy/Immune Tolerance Primer Library |
|||
| HTAIT-I | Real Time Primers | 1 set | EUR 657.6 |
Human T-Cell Receptor Signaling Primer Library |
|||
| HTCR-I | Real Time Primers | 1 set | EUR 657.6 |
Human T-Cell Development and Differentiation Primer Library |
|||
| HTDD-I | Real Time Primers | 1 set | EUR 657.6 |
Human TGF Beta Signaling Primer Library |
|||
| HTGFB-I | Real Time Primers | 1 set | EUR 657.6 |
Human Th1 and Th2 Cell Differentiation Primer Library |
|||
| HTHD-I | Real Time Primers | 1 set | EUR 657.6 |
Human Tumor Invasion/Metastasis Primer Library |
|||
| HTIM-I | Real Time Primers | 1 set | EUR 540 |
Human Tumor Immunity Primer Library |
|||
| HTIMU-I | Real Time Primers | 1 set | EUR 657.6 |
Human Toll-like Receptor Signaling Primer Library |
|||
| HTLR-I | Real Time Primers | 1 set | EUR 657.6 |
Human TNF Signaling Primer Library |
|||
| HTNF-I | Real Time Primers | 1 set | EUR 657.6 |
Human p53 Signaling Primer Library |
|||
| HTPS-I | Real Time Primers | 1 set | EUR 540 |
Human VEGF Signaling Primer Library |
|||
| HVEGF-I | Real Time Primers | 1 set | EUR 657.6 |
Human WNT Signaling Primer Library |
|||
| HWNT-I | Real Time Primers | 1 set | EUR 657.6 |
Rat Bdnf Signaling Primer Library |
|||
| RBDNF-I | Real Time Primers | 1 set | EUR 774 |
Rat Cytokine Primer Library I |
|||
| RCA-I | Real Time Primers | 1 set | EUR 657.6 |
Rat Cytokine Primer Library II |
|||
| RCA-II | Real Time Primers | 1 set | EUR 657.6 |
Rat NFKappaB Primer Library |
|||
| RNFKB-I | Real Time Primers | 1 set | EUR 774 |
Rat Neurotransmitter Signaling Primer Library |
|||
| RNTS-I | Real Time Primers | 1 set | EUR 774 |
Rat Oxidative Stress Primer Library |
|||
| ROSL-I | Real Time Primers | 1 set | EUR 657.6 |
Rat Gdnf Signaling Primer Library |
|||
| RGDNF-I | Real Time Primers | 1 set | EUR 774 |
Rat Glucose Metabolism Primer Library |
|||
| RGLM-I | Real Time Primers | 1 set | EUR 657.6 |
Rat Integrin Signaling Primer Library |
|||
| RINT-I | Real Time Primers | 1 set | EUR 1008 |
Rat Autophagy Primer Library |
|||
| RATPL-I | Real Time Primers | 1 set | EUR 774 |
Rat Ubiquitin Metabolism Primer Library |
|||
| RUBM-I | Real Time Primers | 1 set | EUR 774 |
ReadiViewâ„¢ Blue Real-Time PCR Visualization Dye *200X* |
|||
| 17300-500Tests | AAT Bioquest | 500 Tests | EUR 97 |
|
Description: ReadiViewâ„¢ Blue Real-Time PCR Visualization Dye is a 200X concentrated, ready-to-use, inert dye that enhances the visibility of real-time PCR reactions for more accurate pipetting and plate loading. |
|||
hsa-mir-521 Real-Time RT-PCR Detection and U6 Calibration Kit |
|||
| 20-abx097601 | Abbexa |
|
|
The research was carried out in the course of the mission of specialised anti-epidemic workforce of the Russian Federation (SAET) within the Republic of Guinea in 2015-2018. Based mostly on sequencing knowledge, LASV-specific assay was developed utilizing artificial MS2-phage-based armored RNA particles, RNA from Lassa virus pressure Josiah, and additional, evaluated in subject situations utilizing samples from sufferers and Mastomys natalensis rodents.