Since 2014, porcine epidemic diarrhea virus (PEDV) has reemerged in Europe. RT-PCR strategies have been described for the detection of PEDV, however none have been validated in keeping with a norm. On this research we described the event and validation of a SYBR™ Inexperienced one-step RT-qPCR in keeping with the French norm NF U47-600, for the detection and quantification of PEDV viral RNA. The strategy was validated from pattern preparation (feces or jejunum) by way of to nucleic acid extraction and RT-qPCR detection. Specificity and sensitivity, restrict of detection (LoD), restrict of quantification (LQ), linearity, intra and inter assay variability had been evaluated utilizing transcribed RNA and fecal and jejunum matrices spiked with virus.
The analytical and diagnostic specificities and sensitivities of this RT-qPCR had been 100% on this research. A LoD of 50 genome copies/5 μl of extract from fecal matrices spiked with virus or RNA transcript and 100 genome copies/5 μl of extract from jejunum matrices spiked with virus had been obtained. The Decrease LQ (LLQ) was 100 genome copies/5 μl and the Higher LQ (ULQ) 108 copies/5 μl. This technique is the primary, validated in accordance a norm for PEDV and should function a worldwide reference technique to harmonize detection and quantification of PEDV viral RNA in each subject and experimental settings.
As well as, AluI minimize the amplicon of the E gene of SARS-CoV-2 producing two fragments of 248 and 97 bp with out chopping to SARS-CoV. The accuracy of the assay was confirmed by sequencing and phylogenetic evaluation. When evaluated on scientific samples confirmed a excessive sensitivity of 95%, specificity of our assay was 100% and scientific efficiency for detection of SARS-CoV-2 compared with different reference assays. In conclusion, within the current research, we efficiently developed a easy technique for molecular detection of SARS-CoV-2 in differentiating with SARS-CoV.
Detection of avian influenza virus and newcastle illness virus by duplex one step RT PCR.
Newcastle illness Virus (NDV), a member of the Paramyxoviridae household, and Influenza virus, from the Orthomyxoviridae household, are two most important avian pathogens that trigger critical financial issues in poultry farming. NDV strains are labeled into three main pathotypes: velogenic, mesogenic, and lentogenic. Avian influenza viruses (AIV) are additionally divided into: low pathogenic (LPAI) and extremely pathogenic (HPAI) strains. Each viruses are enveloped, single stranded, negative-sense RNA viruses which give comparable signs starting from sub-clinical infections to extreme illness, together with loss in egg manufacturing, acute respiratory syndrome, and excessive mortality, relying on their stage of pathogenicity.
This similarity hinders prognosis when based mostly solely on scientific and publish mortem examination. A lot of the at present obtainable molecular detection strategies are additionally pathogenspecific, in order that a couple of RT-PCR is then required to verify or exclude the presence of each pathogens. To beat this drawback, we now have utilized a One Step Duplex RT-PCR technique to tell apart between these two pathogens. The primary goal of the venture was to develop a common, quick, and cheap technique which could possibly be utilized in any veterinary laboratory.
Reducing H+ RT concentrations 10 to 104-fold from producer suggestions improved cDNA predictions (common 0.Eight cycles) and elevated RT-PCR assay effectivity. RT exercise assays and fashions can be utilized to assist assay design and enhance the pace of RT-PCRs. RT kind and focus should be chosen to advertise speedy cDNA synthesis however reduce nonspecific amplification. We display 2-minute one-step RT-PCR of a Zika virus goal utilizing decreased RT concentrations and excessive PCR.
Thermo-Responsive Polymer Capsules in Actual-Time One–Step RT–PCR for Extremely Multiplex RNA Evaluation.
Speedy and easy detection of RNA targets is in excessive demand because of the rising menace of pandemic viruses. One-step real-time, reverse transcription-polymerase chain response (One-step RT-qPCR) utilizing a managed launch system of thermo-responsive supplies is developed on this paper to allow high-fidelity RNA evaluation as suppressing by-products. The nanocapsules, consisting of higher important resolution temperature (UCST) materials and PCR primers, carry or launch the primers relying upon the temperature.
The united states nanocapsules are launched into hydrogel microparticles included with RT primers after which the goal RNA is selectively amplified within the microparticle by way of one-step RT-qPCR. Extreme aspect merchandise are sharply subdued by separating the PCR primers from the RT course of via the microparticles with nanocapsules. As a result of the one-step assay is now carried out in a single microparticle, a number of goal RNAs will be analyzed in a easy RT-qPCR of a number of particles. Dependable 18-plex one-step RT-qPCR is efficiently carried out inside 30 min utilizing single-color fluorescent optics.
This work additionally explains the facile fabrication processes used for the thermo-responsive nanocapsules and hydrogel microparticles by the mixing polymerization technique. Extensible multiplex evaluation of influenza virus demonstrates the versatile makes use of of this one-step RT-qPCR platform.
Growth and Analysis of a One–Step Quantitative RT–PCR Assay for Detection of Lassa Virus.
Lassa fever is a extreme viral hemorrhagic sickness brought on by Lassa virus. Based mostly on estimates, the variety of LASV infections ranges from 300,000 to 500,000 circumstances in endemic areas with a fatality price of 1%. Growth of quick and delicate instruments for the management and prevention of Lassa virus an infection in addition to for scientific diagnostics of Lassa fever are essential. Right here we reported improvement and analysis of a one-step quantitative RT-qPCR assay for the Lassa virus detection – LASV-Fl. This assay is appropriate for the detection of lineages I-IV of Lassa virus.
The restrict of detection of the assay ranged from 103 copies/ml to 105 copies/ml and has 96.4% diagnostic sensitivity, whereas analytical and diagnostic specificities each had been 100%. Serum, complete blood and tissue are appropriate to be used with the assay. The assay accommodates all the mandatory elements to carry out the evaluation, together with an armored optimistic management (ARC+) and an armored inside management (IC).
HDV Real Time RT-PCR Kit |
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GWB-LRB030 | GenWay Biotech | 25 tests | Ask for price |
HGV Real Time RT-PCR Kit |
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GWB-LRB039 | GenWay Biotech | 25 tests | Ask for price |
HGV Real Time RT-PCR Kit |
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GWB-LRB040 | GenWay Biotech | 25 tests | Ask for price |
FMD 2.1 Dry Real‐Time RT‐PCR |
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TC-9117-064 | Tetracore | each | EUR 800 |
HCMV Real Time PCR Kit |
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GWB-LRB009 | GenWay Biotech | 25 tests | Ask for price |
HCMV Real Time PCR Kit |
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GWB-LRB010 | GenWay Biotech | 25 tests | Ask for price |
Human Lung Cancer PCR Primer Library |
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HLUCPL-I | Real Time Primers | 1 set | EUR 657.6 |
Human Schwann Cell PCR Primer Library |
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HSCH-I | Real Time Primers | 1 set | EUR 657.6 |
HEV IV Real Time RT-PCR Kit |
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GWB-LRB031 | GenWay Biotech | 25 tests | Ask for price |
HEV IV Real Time RT-PCR Kit |
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GWB-LRB032 | GenWay Biotech | 25 tests | Ask for price |
ONE STEP RT-PCR REAL TIME KIT |
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MBT128-10R | EWC Diagnostics | 1 unit | EUR 37.4 |
Description: ONE STEP RT-PCR REAL TIME KIT |
ONE STEP RT-PCR REAL TIME KIT |
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MBT128-25R | EWC Diagnostics | 1 unit | EUR 89.72 |
Description: ONE STEP RT-PCR REAL TIME KIT |
ONE STEP RT-PCR REAL TIME KIT |
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MBT128-2X50R | EWC Diagnostics | 1 unit | EUR 314.51 |
Description: ONE STEP RT-PCR REAL TIME KIT |
ONE STEP RT-PCR REAL TIME KIT |
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MBT128-50R | EWC Diagnostics | 1 unit | EUR 165.56 |
Description: ONE STEP RT-PCR REAL TIME KIT |
CP&MP Real Time PCR Kit |
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GWB-LRB005 | GenWay Biotech | 25 tests | Ask for price |
CP&MP Real Time PCR Kit |
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GWB-LRB006 | GenWay Biotech | 25 tests | Ask for price |
Measles Virus Real Time RT-PCR Kit |
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GWB-LRB043 | GenWay Biotech | 25 tests | Ask for price |
Measles Virus Real Time RT-PCR Kit |
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GWB-LRB044 | GenWay Biotech | 25 tests | Ask for price |
Rubella Virus Real Time RT-PCR Kit |
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GWB-LRB045 | GenWay Biotech | 25 tests | Ask for price |
Rubella Virus Real Time RT-PCR Kit |
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GWB-LRB046 | GenWay Biotech | 25 tests | Ask for price |
Chikungunya Virus Real Time RT-PCR Kit |
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GWB-LRB001 | GenWay Biotech | 25 tests | Ask for price |
Chikungunya Virus Real Time RT-PCR Kit |
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GWB-LRB002 | GenWay Biotech | 25 tests | Ask for price |
Monkeypox Virus Real Time PCR Kit |
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PDPS-AR064 | Creative Biogene | 1 unit | Ask for price |
Description: Creative Biogene Monkeypox Virus Real Time PCR Kit is used for the detection of monkeypox Virus in serum or lesion exudate samples by using real time PCR systems. Monkeypox virus (MPV) is a double-stranded DNA, zoonotic virus and a species of the genus Orthopoxvirus in the family Poxviridae. It is one of the human orthopoxviruses that includes variola (VARV), cowpox (CPX), and vaccinia (VACV) viruses. The kit contains a specific ready-to-use system for the detection of the monkeypox Virus. Fluorescence is emitted and measured by the real time systems' optical unit during the PCR. |
Monkeypox Virus Real Time PCR Kit |
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ZD-0076-01 | Gentaur Genprice | 25 tests/kit | Ask for price |
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load. |
Monkeypox Virus Real Time PCR Kit |
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ZD-0076-02 | Gentaur Genprice | 25 tests/kit | Ask for price |
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load. |
Monkeypox Virus Real Time PCR Kit |
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YJC70115NW-25T | Jiangsu Bioperfectus Technologies | 25 tests/kit | Ask for price |
Description: The Bioperfectus Monkeypox Virus Real Time PCR Kit is an in vitro diagnostic test, based on real-time PCR technology, for the detection of DNA from the Monkeypox virus. Specimens can be obtained from human serum, lesion exudate samples and scab. BSL-2 facilities with standard BSL-2 work practices may be used for the test of t he Monkeypox virus. |
VetAlert Johnes Real Time PCR Kit |
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TC-9828-100 | Tetracore | 100 rxns | EUR 460 |
HBV Quantitative Real Time PCR Kit |
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GWB-LRB013 | GenWay Biotech | 25 tests | Ask for price |
HBV Quantitative Real Time PCR Kit |
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GWB-LRB014 | GenWay Biotech | 25 tests | Ask for price |
Toxoplasma Gondii Real Time PCR Kit |
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GWB-LRB049 | GenWay Biotech | 25 tests | Ask for price |
Toxoplasma Gondii Real Time PCR Kit |
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GWB-LRB050 | GenWay Biotech | 25 tests | Ask for price |
HEV I & III Real Time RT-PCR Kit |
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GWB-LRB033 | GenWay Biotech | 25 tests | Ask for price |
HEV I & III Real Time RT-PCR Kit |
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GWB-LRB034 | GenWay Biotech | 25 tests | Ask for price |
HEV General Type Real Time RT-PCR Kit |
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GWB-LRB037 | GenWay Biotech | 25 tests | Ask for price |
HEV General Type Real Time RT-PCR Kit |
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GWB-LRB038 | GenWay Biotech | 25 tests | Ask for price |
AzuraQuant cDNA Synthesis Kit - 100 Reactions |
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AZ-1996 | Real Time Primers | 100 Reactions | EUR 446.4 |
Chlamydia Pheumoniae (CP) Real Time PCR Kit |
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GWB-LRB003 | GenWay Biotech | 25 tests | Ask for price |
Chlamydia Pheumoniae (CP) Real Time PCR Kit |
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GWB-LRB004 | GenWay Biotech | 25 tests | Ask for price |
AzuraQuant cDNA Synthesis Kit - 25 Reactions |
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AZ-1995 | Real Time Primers | 25 Reactions | EUR 183.6 |
BP/MP/CP Multiplex Real Time PCR Kit |
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GWB-LRB007 | GenWay Biotech | 25 tests | Ask for price |
BP/MP/CP Multiplex Real Time PCR Kit |
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GWB-LRB008 | GenWay Biotech | 25 tests | Ask for price |
HBV Quantitative & YMDD Mutation Real Time PCR Kit |
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GWB-LRB015 | GenWay Biotech | 25 tests | Ask for price |
HBV Quantitative & YMDD Mutation Real Time PCR Kit |
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GWB-LRB016 | GenWay Biotech | 25 tests | Ask for price |
HBV Quantitative & YMDD Mutation Real Time PCR Kit |
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GWB-LRB017 | GenWay Biotech | 25 tests | Ask for price |
HBV Quantitative & YMDD Mutation Real Time PCR Kit |
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GWB-LRB018 | GenWay Biotech | 25 tests | Ask for price |
HEV I, II, III, & IV Real Time RT-PCR Kit |
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GWB-LRB035 | GenWay Biotech | 25 tests | Ask for price |
HEV I, II, III, & IV Real Time RT-PCR Kit |
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GWB-LRB036 | GenWay Biotech | 25 tests | Ask for price |
HBV Genetype B,C&D Real Time PCR Kit |
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GWB-LRB023 | GenWay Biotech | 25 tests | Ask for price |
HBV Genetype B,C&D Real Time PCR Kit |
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GWB-LRB024 | GenWay Biotech | 25 tests | Ask for price |
HBV Adefovir-resistant Mutants Real Time PCR Kit |
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GWB-LRB019 | GenWay Biotech | 25 tests | Ask for price |
HBV Adefovir-resistant Mutants Real Time PCR Kit |
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GWB-LRB020 | GenWay Biotech | 25 tests | Ask for price |
Transfusion-transmitted Virus (TTV) Real Time PCR Kit |
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GWB-LRB041 | GenWay Biotech | 25 tests | Ask for price |
Transfusion-transmitted Virus (TTV) Real Time PCR Kit |
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GWB-LRB042 | GenWay Biotech | 25 tests | Ask for price |
HBV Precore Site 1896 Mutation Real Time PCR Kit |
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GWB-LRB021 | GenWay Biotech | 25 tests | Ask for price |
HBV Precore Site 1896 Mutation Real Time PCR Kit |
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GWB-LRB022 | GenWay Biotech | 25 tests | Ask for price |
genesig Real time PCR universal meat detection kit |
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Z-Path-SS-uni-meat | Novacyt Group | 100 tests | EUR 602 |
Description: Universal meat |
Measles Virus and Rubella Virus Real Time RT-PCR Kit |
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GWB-LRB047 | GenWay Biotech | 25 tests | Ask for price |
Measles Virus and Rubella Virus Real Time RT-PCR Kit |
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GWB-LRB048 | GenWay Biotech | 25 tests | Ask for price |
genesig Real time PCR Species specific kit, A.platyrhynchos |
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Z-Path-SS-A.platyrhynchos | Novacyt Group | 100 tests | EUR 602 |
Description: A.platyrhynchos |
ASF 3.0 Dry Real‐Time PCR with IC |
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TC-9113-064 | Tetracore | each | EUR 800 |
genesig Real time PCR Species specific kit for C.hircus |
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Z-Path-SS-C.hircus | Novacyt Group | 100 tests | EUR 602 |
Description: C.hircus |
genesig Real time PCR Species specific kit for E.asinus |
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Z-Path-SS-E.asinus | Novacyt Group | 100 tests | EUR 602 |
Description: E.asinus |
genesig Real time PCR Species specific kit for G.gallus |
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Z-Path-SS-G.gallus | Novacyt Group | 100 tests | EUR 602 |
Description: G.gallus |
genesig Real time PCR Adv Species specific kit, G.gallus |
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Z-Path-SS-G.gallus-Adv | Novacyt Group | 100 tests | EUR 808 |
Description: G.gallus |
genesig Real time PCR Species specific kit for B.bubalis |
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Z-Path-SS-B.bubalis | Novacyt Group | 100 tests | EUR 602 |
Description: B.bubalis |
genesig Real time PCR Species specific kit for Venison |
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Z-Path-SS-Venison | Novacyt Group | 100 tests | EUR 602 |
Description: Venison |
genesig Real time PCR Species specific kit for E.caballus |
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Z-Path-SS-E.caballus | Novacyt Group | 100 tests | EUR 602 |
Description: E.caballus |
genesig Real time PCR Adv Species specific kit, E.caballus |
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Z-Path-SS-E.caballus-Adv | Novacyt Group | 100 tests | EUR 808 |
Description: E.caballus |
genesig Real time PCR Species specific kit for O.anatinus |
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Z-Path-SS-O.anatinus | Novacyt Group | 100 tests | EUR 602 |
Description: O.anatinus |
0.2ML CLEAR STRIP CAPS FOR REAL TIME PCR |
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PCR-2CP-RT-C | CORNING | 125/pk | EUR 267.6 |
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
genesig Real time PCR Adv Species specific kit, A.platyrhyn |
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Z-Path-SS-A.platyrhyncos-Adv | Novacyt Group | 100 tests | EUR 808 |
Description: A.platyrhynchos |
genesig Real time PCR Species specific kit for M.gallopavo |
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Z-Path-SS-M.gallopavo | Novacyt Group | 100 tests | EUR 602 |
Description: M.gallopavo |
genesig Real time PCR Adv Species specific kit, M.gallopavo |
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Z-Path-SS-M.gallopavo-Adv | Novacyt Group | 100 tests | EUR 808 |
Description: M.gallopavo |
genesig Real time PCR Species specific kit for P.africanus |
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Z-Path-SS-P.africanus | Novacyt Group | 100 tests | EUR 602 |
Description: P.africanus |
genesig Real time PCR Species specific kit for A.graveolens |
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Z-Path-SS-A.graveolens | Novacyt Group | 100 tests | EUR 602 |
Description: A.graveolens |
genesig Real time PCR Species specific kit for C.familiaris |
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Z-Path-SS-C.familiaris | Novacyt Group | 100 tests | EUR 602 |
Description: C.familiaris |
genesig Real time PCR Adv Species specific kit,C.familiaris |
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Z-Path-SS-C.familiaris-Adv | Novacyt Group | 100 tests | EUR 808 |
Description: C.familiaris |
ASF 3.0 Dry Real‐Time PCR Dilution Buffer |
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TC-9114-064 | Tetracore | each | EUR 90 |
Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit |
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RR-0478-02 | Liferiver | 25 tests/kit | EUR 1189.2 |
Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. |
genesig Real time PCR Species specific kit for Bos taurus |
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Z-Path-SS-B.taurus | Novacyt Group | 100 tests | EUR 602 |
Description: B.taurus |
genesig Real time PCR Adv Species specific kit, Bos taurus |
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Z-Path-SS-B.taurus-Adv | Novacyt Group | 100 tests | EUR 808 |
Description: B.taurus |
genesig Real time PCR Adv Species specific kit for C.hircus |
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Z-Path-SS-C.hircus-Adv | Novacyt Group | 100 tests | EUR 808 |
Description: C.hircus |
genesig Real time PCR Adv Species specific kit for E.asinus |
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Z-Path-SS-E.asinus-Adv | Novacyt Group | 100 tests | EUR 808 |
Description: E.asinus |
genesig Real time PCR Species specific kit for Ovis aries |
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Z-Path-SS-O.aries | Novacyt Group | 100 tests | EUR 602 |
Description: O.aries |
genesig Real time PCR Adv Species specific kit, Ovis aries |
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Z-Path-SS-O.aries-Adv | Novacyt Group | 100 tests | EUR 808 |
Description: O.aries |
genesig Real time PCR Species specific kit for Sus scrofa |
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Z-Path-SS-S.scrofa | Novacyt Group | 100 tests | EUR 602 |
Description: S.scrofa |
genesig Real time PCR Adv Species specific kit, Sus scrofa |
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Z-Path-SS-S.scrofa-Adv | Novacyt Group | 100 tests | EUR 808 |
Description: S.scrofa |
genesig Real time PCR Adv species specific kit for B.bubalis |
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Z-Path-SS-B.bubalis-Adv | Novacyt Group | 100 tests | EUR 808 |
Description: B.bubalis |
genesig Real time PCR Species specific kit for Universal fis |
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Z-Path-SS-uni-fish-V2.0 | Novacyt Group | 100 tests | EUR 602 |
Description: Universal fish |
Azura PureView 1 Kb DNA Ladder - 100 Lanes |
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AZ-1101 | Real Time Primers | 100 Lanes | EUR 140.4 |
Azura PureView 1 Kb DNA Ladder - 500 Lanes |
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AZ-1105 | Real Time Primers | 500 Lanes | EUR 277.2 |
Azura PureView 1 Kb DNA Ladder - 1000 Lanes |
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AZ-1105-2 | Real Time Primers | 1000 Lanes | EUR 326.4 |
Azura PureView 250bp DNA Ladder - 100 Lanes |
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AZ-1121 | Real Time Primers | 100 Lanes | EUR 140.4 |
Azura PureView 250bp DNA Ladder - 500 Lanes |
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AZ-1125 | Real Time Primers | 500 Lanes | EUR 277.2 |
Azura PureView 250bp DNA Ladder - 1000 Lanes |
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AZ-1125-2 | Real Time Primers | 1000 Lanes | EUR 326.4 |
Azura PureView 100bp DNA Ladder - 100 Lanes |
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AZ-1131 | Real Time Primers | 100 Lanes | EUR 140.4 |
Azura PureView 100bp DNA Ladder - 500 Lanes |
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AZ-1135 | Real Time Primers | 500 Lanes | EUR 277.2 |
Azura PureView 100bp DNA Ladder - 1000 Lanes |
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AZ-1135-2 | Real Time Primers | 1000 Lanes | EUR 326.4 |
Azura PureView 50bp DNA Ladder - 100 Lanes |
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AZ-1151 | Real Time Primers | 100 Lanes | EUR 140.4 |
Azura PureView 50bp DNA Ladder - 500 Lanes |
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AZ-1155 | Real Time Primers | 500 Lanes | EUR 277.2 |
Azura PureView 50bp DNA Ladder - 1000 Lanes |
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AZ-1155-2 | Real Time Primers | 1000 Lanes | EUR 326.4 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 200 Rxn |
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AZ-2301 | Real Time Primers | 200 Rxn | EUR 214.8 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 500 Rxn |
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AZ-2305 | Real Time Primers | 500 Rxn | EUR 384 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 2000 Rxn |
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AZ-2320 | Real Time Primers | 2000 Rxn | EUR 1195.2 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 5000 Rxn |
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AZ-2325 | Real Time Primers | 5000 Rxn | EUR 2817.6 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 200 Rxn |
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AZ-2401 | Real Time Primers | 200 Rxn | EUR 214.8 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 500 Rxn |
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AZ-2405 | Real Time Primers | 500 Rxn | EUR 384 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 2000 Rxn |
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AZ-2420 | Real Time Primers | 2000 Rxn | EUR 1195.2 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 5000 Rxn |
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AZ-2421 | Real Time Primers | 5000 Rxn | EUR 2817.6 |
Mouse Angiogenesis Primer Library |
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MAPL-I | Real Time Primers | 1 set | EUR 540 |
Mouse Adherin - Tight Junction Primer Library |
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MATJ-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Autophagy Primer Library |
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MATPL-I | Real Time Primers | 1 set | EUR 540 |
Mouse Bdnf Signaling Primer Library |
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MBDNF-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse BMP Signaling Primer Library |
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MBMP-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Adipogenesis Primer Library |
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MADG-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse PI3K-AKT Signaling Primer Library |
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MAKT-I | Real Time Primers | 1 set | EUR 657.6 |
Human DNA Repair Primer Library I |
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HDRL-I | Real Time Primers | 1 set | EUR 657.6 |
Human DNA Repair Primer Library II |
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HDRL-II | Real Time Primers | 1 set | EUR 657.6 |
Human Drug Transporter I Primer Library |
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HDTP-I | Real Time Primers | 1 set | EUR 657.6 |
Human Drug Detoxication I |
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HDTX-I | Real Time Primers | 1 set | EUR 657.6 |
Human Drug Detoxication II |
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HDTX-II | Real Time Primers | 1 set | EUR 774 |
Human EGFR Signaling Primer Library |
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HEGFR-I | Real Time Primers | 1 set | EUR 657.6 |
Human Epithelial Mesenchymal Transition Primer Library |
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HEMT-I | Real Time Primers | 1 set | EUR 657.6 |
Human Endothelial Cell Primer Library |
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HEND-I | Real Time Primers | 1 set | EUR 657.6 |
Human ERBB Signaling Primer Library |
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HERBB-I | Real Time Primers | 1 set | EUR 657.6 |
Human Estrogen Signaling Primer Library |
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HESR-I | Real Time Primers | 1 set | EUR 774 |
Human Actin Cytoskeleton Signaling Primer Library |
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HACS-I | Real Time Primers | 1 set | EUR 657.6 |
Human Adipogenesis Primer Library |
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HADG-I | Real Time Primers | 1 set | EUR 657.6 |
Human Adaptive & Innate Immune Response Primer Library |
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HAIIR-I | Real Time Primers | 1 set | EUR 657.6 |
Human PI3K-AKT Signaling Primer Library |
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HAKT-I | Real Time Primers | 1 set | EUR 540 |
Human Amino Acid Metabolism Primer Library |
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HAMM-I | Real Time Primers | 1 set | EUR 774 |
Human Androgen Signaling Primer Library |
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HAND-I | Real Time Primers | 1 set | EUR 657.6 |
Human Angiogenesis Primer Library |
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HAPL-I | Real Time Primers | 1 set | EUR 540 |
Human Antigen Processing and Presentation Primer Library |
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HAPP-I | Real Time Primers | 1 set | EUR 657.6 |
Human Atherosclerosis Primer Library |
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HATH-I | Real Time Primers | 1 set | EUR 657.6 |
Human Adherin - Tight Junction Primer Library |
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HATJ-I | Real Time Primers | 1 set | EUR 657.6 |
Human Autophagy Primer Library |
|||
HATPL-I | Real Time Primers | 1 set | EUR 540 |
Human Alzheimer's Disease Primer Library |
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HAZD-I | Real Time Primers | 1 set | EUR 657.6 |
Human B-Cell Receptor Signaling Primer Library |
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HBCR-I | Real Time Primers | 1 set | EUR 657.6 |
Human BDNF Signaling Primer Library |
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HBDNF-I | Real Time Primers | 1 set | EUR 657.6 |
Human Bladder Cancer Primer Library |
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HBLCP-I | Real Time Primers | 1 set | EUR 657.6 |
Human BMP Signaling Primer Library |
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HBMP-I | Real Time Primers | 1 set | EUR 657.6 |
Human Brain Cancer Primer Library |
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HBRC-I | Real Time Primers | 1 set | EUR 657.6 |
Human Breast Cancer Signaling Primer Library |
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HBRCA-I | Real Time Primers | 1 set | EUR 657.6 |
Human Cytokine Primer Library I |
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HCA-I | Real Time Primers | 1 set | EUR 540 |
Human Cytokine Primer Library II |
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HCA-II | Real Time Primers | 1 set | EUR 540 |
Human Cell Adhesion Primer Library |
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HCAD-I | Real Time Primers | 1 set | EUR 774 |
Human cAMP Signaling Primer Library |
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HCAMP-I | Real Time Primers | 1 set | EUR 657.6 |
Human Cancer Pathway Primer Library |
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HCAN-I | Real Time Primers | 1 set | EUR 657.6 |
Human Calcium Regulation Pathway |
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HCAR-I | Real Time Primers | 1 set | EUR 657.6 |
Human Cell Cycle Primer Library |
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HCC-I | Real Time Primers | 1 set | EUR 540 |
Human Colon Cancer Primer Library I |
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HCCP-I | Real Time Primers | 1 set | EUR 657.6 |
Human Colon Cancer Primer Library II |
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HCCP-II | Real Time Primers | 1 set | EUR 657.6 |
Human Cytokine and Chemokine Receptor Primer Library |
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HCCR-I | Real Time Primers | 1 set | EUR 774 |
Human Cancer Driver Gene I Primer Library |
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HCDG-I | Real Time Primers | 1 set | EUR 774 |
Human Cancer Driver Gene II Primer Library |
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HCDG-II | Real Time Primers | 1 set | EUR 774 |
Human Chromatin Regulation Primer Library |
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HCHR-I | Real Time Primers | 1 set | EUR 774 |
Human Oncogene circRNA Library |
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HCirc-Onc-I | Real Time Primers | 1 set | EUR 1125.6 |
Human CREB Signaling Primer Library |
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HCREB-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Notch Signaling Primer Library |
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MNOT-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Neurotransmitter Signaling Primer Library |
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MNTS-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Oncogene Primer Library |
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MONC-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Oxidative Stress Primer Library |
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MOSL-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Apoptosis Primer Library |
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MPA-I | Real Time Primers | 1 set | EUR 540 |
Mouse NFKappaB Primer Library |
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MNFKB-I | Real Time Primers | 1 set | EUR 540 |
Mouse Jak/Stat Signaling Primer Library |
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MJAK-I | Real Time Primers | 1 set | EUR 774 |
Mouse MAP Kinase Signaling Primer Library |
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MMAP-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Myeloid Derived Suppressor Cell Primer Library |
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MMDSC-I | Real Time Primers | 1 set | EUR 657.6 |
Mouse Integrin Signaling Primer Library |
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MINT-I | Real Time Primers | 1 set | EUR 891.6 |
Rat Gdnf Signaling Primer Library |
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RGDNF-I | Real Time Primers | 1 set | EUR 774 |
Rat Glucose Metabolism Primer Library |
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RGLM-I | Real Time Primers | 1 set | EUR 657.6 |
Rat Integrin Signaling Primer Library |
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RINT-I | Real Time Primers | 1 set | EUR 1008 |
Rat Ubiquitin Metabolism Primer Library |
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RUBM-I | Real Time Primers | 1 set | EUR 774 |
Human VEGF Signaling Primer Library |
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HVEGF-I | Real Time Primers | 1 set | EUR 657.6 |
Human WNT Signaling Primer Library |
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HWNT-I | Real Time Primers | 1 set | EUR 657.6 |
Human MAP Kinase Signaling Library |
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HMAP-I | Real Time Primers | 1 set | EUR 657.6 |
Human Myeloid Derived Suppressor Cell Primer Library |
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HMDSC-I | Real Time Primers | 1 set | EUR 657.6 |
Human Melanoma Primer Library |
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HMEL-I | Real Time Primers | 1 set | EUR 657.6 |
Human Necroptosis Primer Library |
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HNCR-I | Real Time Primers | 1 set | EUR 657.6 |
Human NFKappaB Primer Library |
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HNFKB-I | Real Time Primers | 1 set | EUR 540 |
Human NK Cell Mediated Toxicity Primer Library |
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HNKT-I | Real Time Primers | 1 set | EUR 657.6 |
Human Nitric Oxide Signaling Primer Library |
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HNOS-I | Real Time Primers | 1 set | EUR 657.6 |
Human Notch Signaling Pathway |
|||
HNOT-I | Real Time Primers | 1 set | EUR 657.6 |
Human Neurotransmitter Signaling Primer Library |
|||
HNTS-I | Real Time Primers | 1 set | EUR 657.6 |
Human Oncogene Primer Library |
|||
HONC-I | Real Time Primers | 1 set | EUR 657.6 |
Human Oxidative Stress Primer Library |
|||
HOSL-I | Real Time Primers | 1 set | EUR 540 |
Human Osteogenesis Primer Library |
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HOST-I | Real Time Primers | 1 set | EUR 657.6 |
Human Ovarian Cancer Primer Library |
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HOVCL-I | Real Time Primers | 1 set | EUR 657.6 |
Human Apoptosis Primer Library |
|||
HPA-I | Real Time Primers | 1 set | EUR 540 |
Human Pancreatic Cancer Primer Library |
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HPANC-I | Real Time Primers | 1 set | EUR 657.6 |
Human PDGF Signaling Primer Library |
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HPDGF-I | Real Time Primers | 1 set | EUR 657.6 |
Human Parkinson's Disease Primer Library |
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HPKD-I | Real Time Primers | 1 set | EUR 657.6 |
Human Prostate Cancer Primer Library |
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HPRC-I | Real Time Primers | 1 set | EUR 657.6 |
Human Focal Adhesion Signaling Primer Library |
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HFCA-I | Real Time Primers | 1 set | EUR 657.6 |
Human FGF Receptor Signaling Primer Library |
|||
HFGF-I | Real Time Primers | 1 set | EUR 774 |
Human Fatty Acid and Lipid Metabolism Primer Library |
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HFLM-I | Real Time Primers | 1 set | EUR 657.6 |
Human Gastric Cancer Primer Library |
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HGCPL-I | Real Time Primers | 1 set | EUR 657.6 |
Human GDNF Signaling Primer Library |
|||
HGDNF-I | Real Time Primers | 1 set | EUR 657.6 |
Human Glucose Metabolism Primer Library |
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HGLM-I | Real Time Primers | 1 set | EUR 540 |
The research was carried out in the course of the mission of specialised anti-epidemic workforce of the Russian Federation (SAET) within the Republic of Guinea in 2015-2018. Based mostly on sequencing knowledge, LASV-specific assay was developed utilizing artificial MS2-phage-based armored RNA particles, RNA from Lassa virus pressure Josiah, and additional, evaluated in subject situations utilizing samples from sufferers and Mastomys natalensis rodents.