Since 2014, porcine epidemic diarrhea virus (PEDV) has reemerged in Europe. RT-PCR strategies have been described for the detection of PEDV, however none have been validated in keeping with a norm. On this research we described the event and validation of a SYBR™ Inexperienced one-step RT-qPCR in keeping with the French norm NF U47-600, for the detection and quantification of PEDV viral RNA. The strategy was validated from pattern preparation (feces or jejunum) by way of to nucleic acid extraction and RT-qPCR detection. Specificity and sensitivity, restrict of detection (LoD), restrict of quantification (LQ), linearity, intra and inter assay variability had been evaluated utilizing transcribed RNA and fecal and jejunum matrices spiked with virus.
The analytical and diagnostic specificities and sensitivities of this RT-qPCR had been 100% on this research. A LoD of 50 genome copies/5 μl of extract from fecal matrices spiked with virus or RNA transcript and 100 genome copies/5 μl of extract from jejunum matrices spiked with virus had been obtained. The Decrease LQ (LLQ) was 100 genome copies/5 μl and the Higher LQ (ULQ) 108 copies/5 μl. This technique is the primary, validated in accordance a norm for PEDV and should function a worldwide reference technique to harmonize detection and quantification of PEDV viral RNA in each subject and experimental settings.
As well as, AluI minimize the amplicon of the E gene of SARS-CoV-2 producing two fragments of 248 and 97 bp with out chopping to SARS-CoV. The accuracy of the assay was confirmed by sequencing and phylogenetic evaluation. When evaluated on scientific samples confirmed a excessive sensitivity of 95%, specificity of our assay was 100% and scientific efficiency for detection of SARS-CoV-2 compared with different reference assays. In conclusion, within the current research, we efficiently developed a easy technique for molecular detection of SARS-CoV-2 in differentiating with SARS-CoV.
Detection of avian influenza virus and newcastle illness virus by duplex one step RT PCR.
Newcastle illness Virus (NDV), a member of the Paramyxoviridae household, and Influenza virus, from the Orthomyxoviridae household, are two most important avian pathogens that trigger critical financial issues in poultry farming. NDV strains are labeled into three main pathotypes: velogenic, mesogenic, and lentogenic. Avian influenza viruses (AIV) are additionally divided into: low pathogenic (LPAI) and extremely pathogenic (HPAI) strains. Each viruses are enveloped, single stranded, negative-sense RNA viruses which give comparable signs starting from sub-clinical infections to extreme illness, together with loss in egg manufacturing, acute respiratory syndrome, and excessive mortality, relying on their stage of pathogenicity.
This similarity hinders prognosis when based mostly solely on scientific and publish mortem examination. A lot of the at present obtainable molecular detection strategies are additionally pathogenspecific, in order that a couple of RT-PCR is then required to verify or exclude the presence of each pathogens. To beat this drawback, we now have utilized a One Step Duplex RT-PCR technique to tell apart between these two pathogens. The primary goal of the venture was to develop a common, quick, and cheap technique which could possibly be utilized in any veterinary laboratory.
Reducing H+ RT concentrations 10 to 104-fold from producer suggestions improved cDNA predictions (common 0.Eight cycles) and elevated RT-PCR assay effectivity. RT exercise assays and fashions can be utilized to assist assay design and enhance the pace of RT-PCRs. RT kind and focus should be chosen to advertise speedy cDNA synthesis however reduce nonspecific amplification. We display 2-minute one-step RT-PCR of a Zika virus goal utilizing decreased RT concentrations and excessive PCR.
Thermo-Responsive Polymer Capsules in Actual-Time One–Step RT–PCR for Extremely Multiplex RNA Evaluation.
Speedy and easy detection of RNA targets is in excessive demand because of the rising menace of pandemic viruses. One-step real-time, reverse transcription-polymerase chain response (One-step RT-qPCR) utilizing a managed launch system of thermo-responsive supplies is developed on this paper to allow high-fidelity RNA evaluation as suppressing by-products. The nanocapsules, consisting of higher important resolution temperature (UCST) materials and PCR primers, carry or launch the primers relying upon the temperature.
The united states nanocapsules are launched into hydrogel microparticles included with RT primers after which the goal RNA is selectively amplified within the microparticle by way of one-step RT-qPCR. Extreme aspect merchandise are sharply subdued by separating the PCR primers from the RT course of via the microparticles with nanocapsules. As a result of the one-step assay is now carried out in a single microparticle, a number of goal RNAs will be analyzed in a easy RT-qPCR of a number of particles. Dependable 18-plex one-step RT-qPCR is efficiently carried out inside 30 min utilizing single-color fluorescent optics.
This work additionally explains the facile fabrication processes used for the thermo-responsive nanocapsules and hydrogel microparticles by the mixing polymerization technique. Extensible multiplex evaluation of influenza virus demonstrates the versatile makes use of of this one-step RT-qPCR platform.
Growth and Analysis of a One–Step Quantitative RT–PCR Assay for Detection of Lassa Virus.
Lassa fever is a extreme viral hemorrhagic sickness brought on by Lassa virus. Based mostly on estimates, the variety of LASV infections ranges from 300,000 to 500,000 circumstances in endemic areas with a fatality price of 1%. Growth of quick and delicate instruments for the management and prevention of Lassa virus an infection in addition to for scientific diagnostics of Lassa fever are essential. Right here we reported improvement and analysis of a one-step quantitative RT-qPCR assay for the Lassa virus detection – LASV-Fl. This assay is appropriate for the detection of lineages I-IV of Lassa virus.
The restrict of detection of the assay ranged from 103 copies/ml to 105 copies/ml and has 96.4% diagnostic sensitivity, whereas analytical and diagnostic specificities each had been 100%. Serum, complete blood and tissue are appropriate to be used with the assay. The assay accommodates all the mandatory elements to carry out the evaluation, together with an armored optimistic management (ARC+) and an armored inside management (IC).
lyophilised real-time PCR Master Mix for dual labeled probes |
|||
| M-PCR-156L | MiTeGen | 960 reactions x 20 µl | EUR 900.68 |
|
Description: lyophilised real-time PCR Master Mix for dual labeled probes |
|||
lyophilised real-time PCR Master Mix for dual labeled probes |
|||
| M-PCR-156S | MiTeGen | 192 reactions x 20 µl | EUR 225.3 |
|
Description: lyophilised real-time PCR Master Mix for dual labeled probes |
|||
Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit |
|||
| RR-0478-02 | Liferiver | 25 tests/kit | EUR 1189.2 |
|
Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. |
|||
0,1ML THIN WALL TUBES 8 PER STRIP. CLEAR & REAL TIME STRIP CAPS |
|||
| PCR-0108-LP-RT-C | CORNING | 125/pk | EUR 850.8 |
|
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
|||
0,1ML THIN WALL TUBES 8 PER STRIP. WHITE & REAL TIME STRIP CAPS |
|||
| PCR-0108-LP-RT-W | CORNING | 125/pk | EUR 924 |
|
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
|||
hsa-mir-521 Real-Time RT-PCR Detection and U6 Calibration Kit |
|||
| 20-abx097601 | Abbexa |
|
|
TurboQ Real-time PCR Machine with Laptop |
|||
| MOL2700 | Scientific Laboratory Supplies | EACH | EUR 30805.08 |
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
|||
| M-PCR-173L | MiTeGen | 960 reactions x 20 µl | EUR 789.36 |
|
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
|||
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
|||
| M-PCR-173S | MiTeGen | 192 reactions x 20 µl | EUR 197.34 |
|
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
|||
dsGreen for Real-Time PCR, 100×, 2 mL |
|||
| 61010 | Lumiprobe | 2 mL | EUR 435.6 |
dsGreen for Real-Time PCR, 100×, 1 mL |
|||
| 41010 | Lumiprobe | 1 mL | EUR 298.8 |
dsGreen for Real-Time PCR, 100×, 10 mL |
|||
| 81010 | Lumiprobe | 10 mL | EUR 1042.8 |
dsGreen for Real-Time PCR, 100×, 5 mL |
|||
| 71010 | Lumiprobe | 5 mL | EUR 750 |
PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (mecA) |
|||
| 31036 | Biotium | 1kit | EUR 478.8 |
|
Description: Minimum order quantity: 1 unit of 1kit |
|||
PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA) |
|||
| 31050 | Biotium | 1kit | EUR 513.6 |
|
Description: Minimum order quantity: 1 unit of 1kit |
|||
PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly) |
|||
| 31051 | Biotium | 1kit | EUR 478.8 |
|
Description: Minimum order quantity: 1 unit of 1kit |
|||
PMA Real-Time PCR Bacterial Viability Kit - Legionella pneumophila (mip) |
|||
| 31053 | Biotium | 1kit | EUR 513.6 |
|
Description: Minimum order quantity: 1 unit of 1kit |
|||
hsa-let-7a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097603 | Abbexa |
|
|
hsa-let-7b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097604 | Abbexa |
|
|
hsa-let-7c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097605 | Abbexa |
|
|
hsa-let-7d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097606 | Abbexa |
|
|
hsa-let-7e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097607 | Abbexa |
|
|
hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097608 | Abbexa |
|
|
hsa-let-7g Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097609 | Abbexa |
|
|
hsa-let-7i Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097610 | Abbexa |
|
|
hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097611 | Abbexa |
|
|
hsa-mir-1 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097612 | Abbexa |
|
|
hsa-mir-7 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097613 | Abbexa |
|
|
hsa-mir-9 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097614 | Abbexa |
|
|
hsa-mir-9* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097615 | Abbexa |
|
|
hsa-mir-10a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097616 | Abbexa |
|
|
hsa-mir-10b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097617 | Abbexa |
|
|
hsa-mir-10a* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097618 | Abbexa |
|
|
hsa-mir-15a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097619 | Abbexa |
|
|
hsa-mir-15b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097620 | Abbexa |
|
|
hsa-mir-16 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097622 | Abbexa |
|
|
hsa-mir-18a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097625 | Abbexa |
|
|
hsa-mir-18b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097626 | Abbexa |
|
|
hsa-mir-19a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097627 | Abbexa |
|
|
hsa-mir-19b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097628 | Abbexa |
|
|
hsa-mir-20a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097629 | Abbexa |
|
|
hsa-mir-20b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097630 | Abbexa |
|
|
hsa-mir-21 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097631 | Abbexa |
|
|
hsa-mir-22 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097632 | Abbexa |
|
|
hsa-mir-23a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097633 | Abbexa |
|
|
hsa-mir-23b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097634 | Abbexa |
|
|
hsa-mir-25 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097636 | Abbexa |
|
|
hsa-mir-26a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097637 | Abbexa |
|
|
hsa-mir-26b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097638 | Abbexa |
|
|
hsa-mir-27a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097639 | Abbexa |
|
|
hsa-mir-27b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097640 | Abbexa |
|
|
hsa-mir-29a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097642 | Abbexa |
|
|
hsa-mir-29b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097643 | Abbexa |
|
|
hsa-mir-29c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097644 | Abbexa |
|
|
hsa-mir-30a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097645 | Abbexa |
|
|
hsa-mir-30b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097647 | Abbexa |
|
|
hsa-mir-30c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097648 | Abbexa |
|
|
hsa-mir-30d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097650 | Abbexa |
|
|
hsa-mir-30e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097651 | Abbexa |
|
|
hsa-mir-31 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097652 | Abbexa |
|
|
hsa-mir-32 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097653 | Abbexa |
|
|
hsa-mir-33a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097654 | Abbexa |
|
|
hsa-mir-34a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097657 | Abbexa |
|
|
hsa-mir-34b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097659 | Abbexa |
|
|
hsa-mir-92a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097661 | Abbexa |
|
|
hsa-mir-92b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097662 | Abbexa |
|
|
hsa-mir-93 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097663 | Abbexa |
|
|
hsa-mir-95 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097664 | Abbexa |
|
|
hsa-mir-96 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097665 | Abbexa |
|
|
hsa-mir-98 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097666 | Abbexa |
|
|
hsa-mir-99a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097667 | Abbexa |
|
|
hsa-mir-99b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097669 | Abbexa |
|
|
hsa-mir-100 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097671 | Abbexa |
|
|
hsa-mir-101 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097673 | Abbexa |
|
|
hsa-mir-103 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097675 | Abbexa |
|
|
hsa-mir-105 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097676 | Abbexa |
|
|
hsa-mir-106a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097677 | Abbexa |
|
|
hsa-mir-106b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097678 | Abbexa |
|
|
hsa-mir-107 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097679 | Abbexa |
|
|
hsa-mir-124 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097680 | Abbexa |
|
|
hsa-mir-125a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097681 | Abbexa |
|
|
hsa-mir-127 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097686 | Abbexa |
|
|
hsa-mir-129 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097689 | Abbexa |
|
|
hsa-mir-130a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097690 | Abbexa |
|
|
hsa-mir-130b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097691 | Abbexa |
|
|
hsa-mir-132 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097692 | Abbexa |
|
|
hsa-mir-133a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097693 | Abbexa |
|
|
hsa-mir-133b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097694 | Abbexa |
|
|
hsa-mir-134 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097695 | Abbexa |
|
|
hsa-mir-135b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097696 | Abbexa |
|
|
hsa-mir-137 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097699 | Abbexa |
|
|
hsa-mir-138 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097700 | Abbexa |
|
|
hsa-mir-139 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097701 | Abbexa |
|
|
hsa-mir-140 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097702 | Abbexa |
|
|
hsa-mir-141 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097703 | Abbexa |
|
|
hsa-mir-143 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097706 | Abbexa |
|
|
hsa-mir-145 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097708 | Abbexa |
|
|
hsa-mir-146a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097709 | Abbexa |
|
|
hsa-mir-149 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097711 | Abbexa |
|
|
hsa-mir-150 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097712 | Abbexa |
|
|
hsa-mir-151 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097714 | Abbexa |
|
|
hsa-mir-152 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097715 | Abbexa |
|
|
hsa-mir-153 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097716 | Abbexa |
|
|
hsa-mir-154 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097717 | Abbexa |
|
|
hsa-mir-181a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097719 | Abbexa |
|
|
hsa-mir-181b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097720 | Abbexa |
|
|
hsa-mir-181c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097721 | Abbexa |
|
|
hsa-mir-181d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097722 | Abbexa |
|
|
hsa-mir-182 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097723 | Abbexa |
|
|
hsa-mir-183 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097724 | Abbexa |
|
|
hsa-mir-184 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097725 | Abbexa |
|
|
hsa-mir-185 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097726 | Abbexa |
|
|
hsa-mir-190a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097728 | Abbexa |
|
|
hsa-mir-190b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097729 | Abbexa |
|
|
hsa-mir-192 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097731 | Abbexa |
|
|
hsa-mir-193a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097732 | Abbexa |
|
|
hsa-mir-193b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097733 | Abbexa |
|
|
hsa-mir-194 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097734 | Abbexa |
|
|
hsa-mir-195 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097735 | Abbexa |
|
|
hsa-mir-197 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097738 | Abbexa |
|
|
hsa-mir-198 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097739 | Abbexa |
|
|
hsa-mir-199a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097740 | Abbexa |
|
|
hsa-mir-199b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097741 | Abbexa |
|
|
hsa-mir-200a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097742 | Abbexa |
|
|
hsa-mir-200b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097743 | Abbexa |
|
|
hsa-mir-202 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097745 | Abbexa |
|
|
hsa-mir-203 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097746 | Abbexa |
|
|
hsa-mir-204 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097747 | Abbexa |
|
|
hsa-mir-205 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097748 | Abbexa |
|
|
hsa-mir-206 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097749 | Abbexa |
|
|
hsa-mir-208a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097750 | Abbexa |
|
|
hsa-mir-208b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097751 | Abbexa |
|
|
hsa-mir-211 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097753 | Abbexa |
|
|
hsa-mir-212 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097754 | Abbexa |
|
|
hsa-mir-214 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097755 | Abbexa |
|
|
hsa-mir-215 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097756 | Abbexa |
|
|
hsa-mir-217 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097757 | Abbexa |
|
|
hsa-mir-218 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097758 | Abbexa |
|
|
hsa-mir-219 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097759 | Abbexa |
|
|
hsa-mir-223 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097764 | Abbexa |
|
|
hsa-mir-224 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097765 | Abbexa |
|
|
hsa-mir-298 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097768 | Abbexa |
|
|
hsa-mir-299 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097769 | Abbexa |
|
|
hsa-mir-320a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097774 | Abbexa |
|
|
hsa-mir-320b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097775 | Abbexa |
|
|
hsa-mir-320c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097776 | Abbexa |
|
|
hsa-mir-326 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097777 | Abbexa |
|
|
hsa-mir-328 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097778 | Abbexa |
|
|
hsa-mir-331 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097780 | Abbexa |
|
|
hsa-mir-335 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097781 | Abbexa |
|
|
hsa-mir-338 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097782 | Abbexa |
|
|
hsa-mir-373 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097786 | Abbexa |
|
|
hsa-mir-365 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097789 | Abbexa |
|
|
hsa-mir-370 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097790 | Abbexa |
|
|
hsa-mir-373 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097792 | Abbexa |
|
|
hsa-mir-375 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097794 | Abbexa |
|
|
hsa-mir-378 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097795 | Abbexa |
|
|
hsa-mir-381 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097796 | Abbexa |
|
|
hsa-mir-382 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097797 | Abbexa |
|
|
hsa-mir-421 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097798 | Abbexa |
|
|
hsa-mir-422a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097799 | Abbexa |
|
|
hsa-mir-425 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097801 | Abbexa |
|
|
hsa-mir-429 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097802 | Abbexa |
|
|
hsa-mir-431 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097803 | Abbexa |
|
|
hsa-mir-432 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097804 | Abbexa |
|
|
hsa-mir-433 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097805 | Abbexa |
|
|
hsa-mir-451a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097808 | Abbexa |
|
|
hsa-mir-484 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097813 | Abbexa |
|
|
hsa-mir-487b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097815 | Abbexa |
|
|
hsa-mir-492 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097816 | Abbexa |
|
|
hsa-mir-493 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097817 | Abbexa |
|
|
hsa-mir-494 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097818 | Abbexa |
|
|
hsa-mir-497 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097819 | Abbexa |
|
|
hsa-mir-499 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097820 | Abbexa |
|
|
hsa-mir-503 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097821 | Abbexa |
|
|
hsa-mir-504 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097822 | Abbexa |
|
|
hsa-mir-515 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097823 | Abbexa |
|
|
hsa-mir-519a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097824 | Abbexa |
|
|
hsa-mir-568 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097829 | Abbexa |
|
|
hsa-mir-577 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097831 | Abbexa |
|
|
hsa-mir-603 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097833 | Abbexa |
|
|
hsa-mir-607 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097834 | Abbexa |
|
|
hsa-mir-608 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097835 | Abbexa |
|
|
hsa-mir-609 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097836 | Abbexa |
|
|
hsa-mir-612 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097837 | Abbexa |
|
|
hsa-mir-618 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097839 | Abbexa |
|
|
hsa-mir-623 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097840 | Abbexa |
|
|
hsa-mir-630 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097844 | Abbexa |
|
|
hsa-mir-633 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097845 | Abbexa |
|
|
hsa-mir-635 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097846 | Abbexa |
|
|
hsa-mir-639 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097847 | Abbexa |
|
|
hsa-mir-640 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097848 | Abbexa |
|
|
hsa-mir-663a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097849 | Abbexa |
|
|
hsa-mir-675 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097850 | Abbexa |
|
|
hsa-mir-933 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097855 | Abbexa |
|
|
hsa-mir-940 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097856 | Abbexa |
|
|
hsa-mir-944 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097857 | Abbexa |
|
|
hsa-mir-1180 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097858 | Abbexa |
|
|
hsa-mir-1178 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097859 | Abbexa |
|
|
The research was carried out in the course of the mission of specialised anti-epidemic workforce of the Russian Federation (SAET) within the Republic of Guinea in 2015-2018. Based mostly on sequencing knowledge, LASV-specific assay was developed utilizing artificial MS2-phage-based armored RNA particles, RNA from Lassa virus pressure Josiah, and additional, evaluated in subject situations utilizing samples from sufferers and Mastomys natalensis rodents.