A one-step real-time RT-PCR helps to identify mixed rotavirus infections in Mexico.

A one-step real-time RT-PCR helps to identify mixed…

Rotaviruses proceed being crucial pathogens accountable of diarrhea in younger kids worldwide. Seminested reverse transcription polymerase chain response (RT-PCR) is used to find out rotavirus genotype; nevertheless, this method employs multistep procedures. The actual-time RT-PCR…
Development of a New Internally Controlled One-Step Real-Time RT-PCR for the Molecular Detection of Enterovirus A71 in Africa and Madagascar

Development of a New Internally Controlled One-Step Real-Time…

Enterovirus A71 (EV-A71) is a number one reason behind hand-foot-and-mouth illness (HFMD) and might be related to extreme neurological problems. EV-A71 strains might be labeled into seven genogroups, A-H, on the idea of the VP1…
Microdroplet-based one-step RT-PCR for ultrahigh throughput single-cell multiplex gene expression analysis and rare cell detection

Microdroplet-based one-step RT-PCR for ultrahigh throughput single-cell multiplex…

Gene expression evaluation of particular person cells permits characterization of heterogeneous and uncommon cell populations, but widespread implementation of current single-cell gene evaluation strategies has been hindered because of limitations in scale, ease, and price.…
A one-step real-time RT-PCR assay for simultaneous typing of SARS-CoV-2 mutations associated with the E484K and N501Y spike protein amino-acid substitutions

A one-step real-time RT-PCR assay for simultaneous typing…

The emergence of SARS-CoV-2 mutations ensuing within the S protein amino-acid substitutions N501Y and E484Ok, which have been related to enhanced transmissibility and immune escape, respectively, necessitates speedy actions, for which their speedy identification is…